Cyclotron Production of High–Specific Activity Co and In Vivo Evaluation of the Stability of Co Metal-Chelate-Peptide Complexes
This work describes the production of high–specific activity 55 Co and the evaluation of the stability of 55 Co-metal-chelate-peptide complexes in vivo. 55 Co was produced via the 58 Ni(p,α) 55 Co reaction and purified using anion exchange chromatography with an average recovery of 92% and an averag...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2015-10-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2015.00025 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841563124806713344 |
|---|---|
| author | Tara Mastren Bernadette V. Marquez Deborah E. Sultan Elizabeth Bollinger Paul Eisenbeis Tom Voller Suzanne E. Lapi |
| author_facet | Tara Mastren Bernadette V. Marquez Deborah E. Sultan Elizabeth Bollinger Paul Eisenbeis Tom Voller Suzanne E. Lapi |
| author_sort | Tara Mastren |
| collection | DOAJ |
| description | This work describes the production of high–specific activity 55 Co and the evaluation of the stability of 55 Co-metal-chelate-peptide complexes in vivo. 55 Co was produced via the 58 Ni(p,α) 55 Co reaction and purified using anion exchange chromatography with an average recovery of 92% and an average specific activity of 1.96 GBq/μmol. 55 Co-DO3A and 55 Co-NO2A peptide complexes were radiolabeled at 3.7 MBq/μg and injected into HCT-116 tumor xenografted mice. Positron emission tomography (PET) and biodistribution studies were performed at 24 and 48 hours postinjection and compared to those of 55 CoCl 2 . Both 55 Co-metal-chelate complexes demonstrated good in vivo stability by reducing the radiotracers’ uptake in the liver by sixfold at 24 hours with ˜ 1% ID/g and at 48 hours with ˜ 0.5% ID/g and reducing uptake in the heart by fourfold at 24 hours with ˜ 0.7% ID/g and sevenfold at 48 hours with ˜ 0.35% ID/g. These results support the use of 55 Co as a promising new radiotracer for PET imaging of cancer and other diseases. |
| format | Article |
| id | doaj-art-22e86e65a90a4bf8b4595b1459b1dd4a |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2015-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-22e86e65a90a4bf8b4595b1459b1dd4a2025-01-03T00:11:32ZengSAGE PublishingMolecular Imaging1536-01212015-10-011410.2310/7290.2015.0002510.2310_7290.2015.00025Cyclotron Production of High–Specific Activity Co and In Vivo Evaluation of the Stability of Co Metal-Chelate-Peptide ComplexesTara MastrenBernadette V. MarquezDeborah E. SultanElizabeth BollingerPaul EisenbeisTom VollerSuzanne E. LapiThis work describes the production of high–specific activity 55 Co and the evaluation of the stability of 55 Co-metal-chelate-peptide complexes in vivo. 55 Co was produced via the 58 Ni(p,α) 55 Co reaction and purified using anion exchange chromatography with an average recovery of 92% and an average specific activity of 1.96 GBq/μmol. 55 Co-DO3A and 55 Co-NO2A peptide complexes were radiolabeled at 3.7 MBq/μg and injected into HCT-116 tumor xenografted mice. Positron emission tomography (PET) and biodistribution studies were performed at 24 and 48 hours postinjection and compared to those of 55 CoCl 2 . Both 55 Co-metal-chelate complexes demonstrated good in vivo stability by reducing the radiotracers’ uptake in the liver by sixfold at 24 hours with ˜ 1% ID/g and at 48 hours with ˜ 0.5% ID/g and reducing uptake in the heart by fourfold at 24 hours with ˜ 0.7% ID/g and sevenfold at 48 hours with ˜ 0.35% ID/g. These results support the use of 55 Co as a promising new radiotracer for PET imaging of cancer and other diseases.https://doi.org/10.2310/7290.2015.00025 |
| spellingShingle | Tara Mastren Bernadette V. Marquez Deborah E. Sultan Elizabeth Bollinger Paul Eisenbeis Tom Voller Suzanne E. Lapi Cyclotron Production of High–Specific Activity Co and In Vivo Evaluation of the Stability of Co Metal-Chelate-Peptide Complexes Molecular Imaging |
| title | Cyclotron Production of High–Specific Activity Co and In Vivo Evaluation of the Stability of Co Metal-Chelate-Peptide Complexes |
| title_full | Cyclotron Production of High–Specific Activity Co and In Vivo Evaluation of the Stability of Co Metal-Chelate-Peptide Complexes |
| title_fullStr | Cyclotron Production of High–Specific Activity Co and In Vivo Evaluation of the Stability of Co Metal-Chelate-Peptide Complexes |
| title_full_unstemmed | Cyclotron Production of High–Specific Activity Co and In Vivo Evaluation of the Stability of Co Metal-Chelate-Peptide Complexes |
| title_short | Cyclotron Production of High–Specific Activity Co and In Vivo Evaluation of the Stability of Co Metal-Chelate-Peptide Complexes |
| title_sort | cyclotron production of high specific activity co and in vivo evaluation of the stability of co metal chelate peptide complexes |
| url | https://doi.org/10.2310/7290.2015.00025 |
| work_keys_str_mv | AT taramastren cyclotronproductionofhighspecificactivitycoandinvivoevaluationofthestabilityofcometalchelatepeptidecomplexes AT bernadettevmarquez cyclotronproductionofhighspecificactivitycoandinvivoevaluationofthestabilityofcometalchelatepeptidecomplexes AT deborahesultan cyclotronproductionofhighspecificactivitycoandinvivoevaluationofthestabilityofcometalchelatepeptidecomplexes AT elizabethbollinger cyclotronproductionofhighspecificactivitycoandinvivoevaluationofthestabilityofcometalchelatepeptidecomplexes AT pauleisenbeis cyclotronproductionofhighspecificactivitycoandinvivoevaluationofthestabilityofcometalchelatepeptidecomplexes AT tomvoller cyclotronproductionofhighspecificactivitycoandinvivoevaluationofthestabilityofcometalchelatepeptidecomplexes AT suzanneelapi cyclotronproductionofhighspecificactivitycoandinvivoevaluationofthestabilityofcometalchelatepeptidecomplexes |