Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
Platycodon grandiflorum has long been used as a traditional oriental medicine for respiratory disorder. Platycodin D (PD) is known as the main component isolated from the root of PG. A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/231293 |
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| author | Tae-Hyun Kim Byung Eui Lee Eun Joo Kim Yong Seok Choi Keun-Sung Lee Hak Rim Kim Hyung-Gun Kim |
| author_facet | Tae-Hyun Kim Byung Eui Lee Eun Joo Kim Yong Seok Choi Keun-Sung Lee Hak Rim Kim Hyung-Gun Kim |
| author_sort | Tae-Hyun Kim |
| collection | DOAJ |
| description | Platycodon grandiflorum has long been used as a traditional oriental medicine for respiratory disorder. Platycodin D (PD) is known as the main component isolated from the root of PG. A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitation of PD in rat plasma. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization and multiple reaction monitoring in positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of PD were linear over the concentration range of 50–10,000 ng/mL in rat plasma. The coefficient of variation and relative error at five QC levels were 1.0 to 8.8% and 0.7 to 8.7%, respectively. After a single oral administration of 500 mg/kg and a single intravenous administration of 25 mg/kg of 3% PD extract (a PG extract including 3% of PD), platycodin D and platycodin D3 were detected and pharmacokinetic parameters were estimated. The oral bioavailability of platycodin D and platycodin D3 was 0.29% and 1.35% in rats at 500 mg/kg of 3% PD extract of PG, respectively. The present method can be applied to pharmacokinetic analysis of platycodins and platycosides of the PG. |
| format | Article |
| id | doaj-art-22d2e64c10054a6a9dce745ce9fa54cf |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-22d2e64c10054a6a9dce745ce9fa54cf2025-02-03T05:47:47ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/231293231293Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass SpectrometryTae-Hyun Kim0Byung Eui Lee1Eun Joo Kim2Yong Seok Choi3Keun-Sung Lee4Hak Rim Kim5Hyung-Gun Kim6Bioresourres Regional Innovation Center, Soonchunhyang University, Asan 336-745, Republic of KoreaIndustry-Academy Cooperation Foundation, Soonchunhyang University, Asan 336-745, Republic of KoreaKorea Institute of Toxicology, Korea Research Institute of Chemical Technology, Daejeon 305-343, Republic of KoreaCollege of Pharmacy, Dankook University, Cheonan 330-714, Republic of KoreaDepartment of Pharmacology, College of Medicine, Dankook University, Cheonan 330-714, Republic of KoreaDepartment of Pharmacology, College of Medicine, Dankook University, Cheonan 330-714, Republic of KoreaDepartment of Pharmacology, College of Medicine, Dankook University, Cheonan 330-714, Republic of KoreaPlatycodon grandiflorum has long been used as a traditional oriental medicine for respiratory disorder. Platycodin D (PD) is known as the main component isolated from the root of PG. A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitation of PD in rat plasma. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization and multiple reaction monitoring in positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of PD were linear over the concentration range of 50–10,000 ng/mL in rat plasma. The coefficient of variation and relative error at five QC levels were 1.0 to 8.8% and 0.7 to 8.7%, respectively. After a single oral administration of 500 mg/kg and a single intravenous administration of 25 mg/kg of 3% PD extract (a PG extract including 3% of PD), platycodin D and platycodin D3 were detected and pharmacokinetic parameters were estimated. The oral bioavailability of platycodin D and platycodin D3 was 0.29% and 1.35% in rats at 500 mg/kg of 3% PD extract of PG, respectively. The present method can be applied to pharmacokinetic analysis of platycodins and platycosides of the PG.http://dx.doi.org/10.1155/2014/231293 |
| spellingShingle | Tae-Hyun Kim Byung Eui Lee Eun Joo Kim Yong Seok Choi Keun-Sung Lee Hak Rim Kim Hyung-Gun Kim Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry The Scientific World Journal |
| title | Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry |
| title_full | Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry |
| title_fullStr | Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry |
| title_full_unstemmed | Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry |
| title_short | Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry |
| title_sort | determination of platycodin d and platycodin d3 in rat plasma using liquid chromatography tandem mass spectrometry |
| url | http://dx.doi.org/10.1155/2014/231293 |
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