Role of KDM2B epigenetic factor in regulating calcium signaling in prostate cancer cells
KDM2B, a histone lysine demethylase, is expressed in a plethora of cancers. Earlier studies from our group, have showcased that overexpression of KDM2B in the human prostate cancer cell line DU-145 is associated with cell adhesion, actin reorganization, and improved cancer cell migration. In additio...
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| Format: | Article |
| Language: | English |
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Springer
2024-07-01
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| Series: | Saudi Pharmaceutical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016424001592 |
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| author | Evangelia Pantazaka Saad Alkahtani Saud Alarifi Abdullah A. Alkahtane Christos Stournaras Galatea Kallergi |
| author_facet | Evangelia Pantazaka Saad Alkahtani Saud Alarifi Abdullah A. Alkahtane Christos Stournaras Galatea Kallergi |
| author_sort | Evangelia Pantazaka |
| collection | DOAJ |
| description | KDM2B, a histone lysine demethylase, is expressed in a plethora of cancers. Earlier studies from our group, have showcased that overexpression of KDM2B in the human prostate cancer cell line DU-145 is associated with cell adhesion, actin reorganization, and improved cancer cell migration. In addition, we have previously examined changes of cytosolic Ca2+, regulated by the pore-forming proteins ORAI and the Ca2+ sensing stromal interaction molecules (STIM), via store-operated Ca2+ entry (SOCE) in wild-type DU-145. This study sought to evaluate the impact of KDM2B overexpression on the expression of key molecules (SGK1, Nhe1, Orai1, Stim1) and SOCE. Furthermore, this is the first study to evaluate KDM2B expression in circulating tumor cells (CTCs) from patients with prostate cancer. mRNA levels for SGK1, Nhe1, Orai1, and Stim1 were quantified by RT-PCR. Calcium signals were measured in KDM2B-overexpressing DU-145 cells, loaded with Fura-2. Blood samples from 22 prostate cancer cases were scrutinized for KDM2B expression using immunofluorescence staining and the VyCAP system. KDM2B overexpression in DU-145 cells increased Orai1, Stim1, and Nhe1 mRNA levels and significantly decreased Ca2+ release. KDM2B expression was examined in 22 prostate cancer patients. CTCs were identified in 45 % of these patients. 80 % of the cytokeratin (CK)-positive patients and 63 % of the total examined CTCs exhibited the (CK + KDM2B + CD45−) phenotype. To conclude, this study is the first to report increased expression of KDM2B in CTCs from patients with prostate cancer, bridging in vitro and preclinical assessments on the potentially crucial role of KDM2B on migration, invasiveness, and ultimately metastasis in prostate cancer. |
| format | Article |
| id | doaj-art-2263f8266dc440a09d3de8b8d363e5e4 |
| institution | Kabale University |
| issn | 1319-0164 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj-art-2263f8266dc440a09d3de8b8d363e5e42025-08-20T03:54:48ZengSpringerSaudi Pharmaceutical Journal1319-01642024-07-0132710210910.1016/j.jsps.2024.102109Role of KDM2B epigenetic factor in regulating calcium signaling in prostate cancer cellsEvangelia Pantazaka0Saad Alkahtani1Saud Alarifi2Abdullah A. Alkahtane3Christos Stournaras4Galatea Kallergi5Laboratory of Biochemistry/Metastatic Signaling, Section of Genetics, Cell Biology and Development, Department of Biology, University of Patras, 26504 Patras, GreeceDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Biochemistry, Medical School, University of Crete, Heraklion 71003, GreeceLaboratory of Biochemistry/Metastatic Signaling, Section of Genetics, Cell Biology and Development, Department of Biology, University of Patras, 26504 Patras, Greece; Corresponding author at: Department of Biology, University of Patras, Campus Rio, 26504 Patras, Greece.KDM2B, a histone lysine demethylase, is expressed in a plethora of cancers. Earlier studies from our group, have showcased that overexpression of KDM2B in the human prostate cancer cell line DU-145 is associated with cell adhesion, actin reorganization, and improved cancer cell migration. In addition, we have previously examined changes of cytosolic Ca2+, regulated by the pore-forming proteins ORAI and the Ca2+ sensing stromal interaction molecules (STIM), via store-operated Ca2+ entry (SOCE) in wild-type DU-145. This study sought to evaluate the impact of KDM2B overexpression on the expression of key molecules (SGK1, Nhe1, Orai1, Stim1) and SOCE. Furthermore, this is the first study to evaluate KDM2B expression in circulating tumor cells (CTCs) from patients with prostate cancer. mRNA levels for SGK1, Nhe1, Orai1, and Stim1 were quantified by RT-PCR. Calcium signals were measured in KDM2B-overexpressing DU-145 cells, loaded with Fura-2. Blood samples from 22 prostate cancer cases were scrutinized for KDM2B expression using immunofluorescence staining and the VyCAP system. KDM2B overexpression in DU-145 cells increased Orai1, Stim1, and Nhe1 mRNA levels and significantly decreased Ca2+ release. KDM2B expression was examined in 22 prostate cancer patients. CTCs were identified in 45 % of these patients. 80 % of the cytokeratin (CK)-positive patients and 63 % of the total examined CTCs exhibited the (CK + KDM2B + CD45−) phenotype. To conclude, this study is the first to report increased expression of KDM2B in CTCs from patients with prostate cancer, bridging in vitro and preclinical assessments on the potentially crucial role of KDM2B on migration, invasiveness, and ultimately metastasis in prostate cancer.http://www.sciencedirect.com/science/article/pii/S1319016424001592KDM2BCalcium signalingOrai1Stim1Store-operated Ca2+ entryProstate cancer |
| spellingShingle | Evangelia Pantazaka Saad Alkahtani Saud Alarifi Abdullah A. Alkahtane Christos Stournaras Galatea Kallergi Role of KDM2B epigenetic factor in regulating calcium signaling in prostate cancer cells Saudi Pharmaceutical Journal KDM2B Calcium signaling Orai1 Stim1 Store-operated Ca2+ entry Prostate cancer |
| title | Role of KDM2B epigenetic factor in regulating calcium signaling in prostate cancer cells |
| title_full | Role of KDM2B epigenetic factor in regulating calcium signaling in prostate cancer cells |
| title_fullStr | Role of KDM2B epigenetic factor in regulating calcium signaling in prostate cancer cells |
| title_full_unstemmed | Role of KDM2B epigenetic factor in regulating calcium signaling in prostate cancer cells |
| title_short | Role of KDM2B epigenetic factor in regulating calcium signaling in prostate cancer cells |
| title_sort | role of kdm2b epigenetic factor in regulating calcium signaling in prostate cancer cells |
| topic | KDM2B Calcium signaling Orai1 Stim1 Store-operated Ca2+ entry Prostate cancer |
| url | http://www.sciencedirect.com/science/article/pii/S1319016424001592 |
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