Gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammation
Evidence suggests that a healthy gut microbiome is essential for metabolizing dietary phytochemicals. However, the microbiome’s role in metabolite production and the influence of gut dysbiosis on this process remain unclear. Further, studies on the relationship among gut microbes, metabolites, and b...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2024.2446375 |
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| author | Chrissa Petersen Adhini Kuppuswamy Satheesh Babu Ceres Mattos Della Lucia Henry A. Paz Lisard Iglesias-Carres Ying Zhong Thunder Jalili J David Symons Kartik Shankar Andrew P. Neilson Umesh D. Wankhade Pon Velayutham Anandh Babu |
| author_facet | Chrissa Petersen Adhini Kuppuswamy Satheesh Babu Ceres Mattos Della Lucia Henry A. Paz Lisard Iglesias-Carres Ying Zhong Thunder Jalili J David Symons Kartik Shankar Andrew P. Neilson Umesh D. Wankhade Pon Velayutham Anandh Babu |
| author_sort | Chrissa Petersen |
| collection | DOAJ |
| description | Evidence suggests that a healthy gut microbiome is essential for metabolizing dietary phytochemicals. However, the microbiome’s role in metabolite production and the influence of gut dysbiosis on this process remain unclear. Further, studies on the relationship among gut microbes, metabolites, and biological activities of phytochemicals are limited. We addressed this knowledge gap using strawberry phytochemicals as a model. C57BL/6J mice were fed a standard diet [C]; strawberry-supplemented diet (~2 human servings) [CS]; strawberry-supplemented diet and treated with antibiotics (to deplete gut microbes) [CSA]; high-fat diet (HFD) [HF]; strawberry-supplemented HFD [HS]; and strawberry-supplemented HFD and treated with antibiotics [HSA] for 12 weeks. First, antibiotic treatment suppressed the production of selected metabolites (CSA vs. CS), and p-coumaric acid was identified as a strawberry-derived microbial metabolite. Second, HFD-induced dysbiosis negatively affected metabolite production (HS vs. HF), and hippuric acid was identified as a microbial metabolite in HFD conditions. Third, dietary strawberries improved HFD-induced vascular inflammation (HS vs. HF). However, antibiotic treatment reduced metabolite production and abolished the vascular effects of strawberries (HSA vs. HS), indicating the importance of gut microbes in mediating the vascular benefits of strawberries via metabolites. Fourth, strawberry supplementation decreased Coprobacillus that was positively associated with vascular inflammation, whereas it increased Lachnospiraceae that was negatively associated with vascular inflammation and positively associated with hippuric acid. Fifth, hippuric acid was negatively associated with vascular inflammation. Our study fills in some pieces of the giant puzzle regarding the influence of gut microbes on the biological activities of phytochemicals. HFD-induced gut dysbiosis negatively impacts metabolite production and a strong association exists among gut microbes, strawberry-derived microbial metabolites, and the vascular benefits of dietary strawberries. Further, our study provides significant proof of concept to warrant future research on the use of strawberries as a nutritional strategy to prevent vascular complications. |
| format | Article |
| id | doaj-art-225618d2bd2344018d36c9e0cd4c2700 |
| institution | Kabale University |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-225618d2bd2344018d36c9e0cd4c27002025-01-06T13:38:15ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2024.2446375Gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammationChrissa Petersen0Adhini Kuppuswamy Satheesh Babu1Ceres Mattos Della Lucia2Henry A. Paz3Lisard Iglesias-Carres4Ying Zhong5Thunder Jalili6J David Symons7Kartik Shankar8Andrew P. Neilson9Umesh D. Wankhade10Pon Velayutham Anandh Babu11Department of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT, USAArkansas Children’s Nutrition Center, University of Arkansas for Medical Sciences, Little Rock, AR, USAPlants for Human Health Institute, Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Kannapolis, NC, USAArkansas Children’s Nutrition Center, University of Arkansas for Medical Sciences, Little Rock, AR, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT, USADepartment of Pediatrics, Section of Nutrition, University of Colorado Anschutz Medical Campus, Aurora, CO, USAPlants for Human Health Institute, Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Kannapolis, NC, USAArkansas Children’s Nutrition Center, University of Arkansas for Medical Sciences, Little Rock, AR, USADepartment of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT, USAEvidence suggests that a healthy gut microbiome is essential for metabolizing dietary phytochemicals. However, the microbiome’s role in metabolite production and the influence of gut dysbiosis on this process remain unclear. Further, studies on the relationship among gut microbes, metabolites, and biological activities of phytochemicals are limited. We addressed this knowledge gap using strawberry phytochemicals as a model. C57BL/6J mice were fed a standard diet [C]; strawberry-supplemented diet (~2 human servings) [CS]; strawberry-supplemented diet and treated with antibiotics (to deplete gut microbes) [CSA]; high-fat diet (HFD) [HF]; strawberry-supplemented HFD [HS]; and strawberry-supplemented HFD and treated with antibiotics [HSA] for 12 weeks. First, antibiotic treatment suppressed the production of selected metabolites (CSA vs. CS), and p-coumaric acid was identified as a strawberry-derived microbial metabolite. Second, HFD-induced dysbiosis negatively affected metabolite production (HS vs. HF), and hippuric acid was identified as a microbial metabolite in HFD conditions. Third, dietary strawberries improved HFD-induced vascular inflammation (HS vs. HF). However, antibiotic treatment reduced metabolite production and abolished the vascular effects of strawberries (HSA vs. HS), indicating the importance of gut microbes in mediating the vascular benefits of strawberries via metabolites. Fourth, strawberry supplementation decreased Coprobacillus that was positively associated with vascular inflammation, whereas it increased Lachnospiraceae that was negatively associated with vascular inflammation and positively associated with hippuric acid. Fifth, hippuric acid was negatively associated with vascular inflammation. Our study fills in some pieces of the giant puzzle regarding the influence of gut microbes on the biological activities of phytochemicals. HFD-induced gut dysbiosis negatively impacts metabolite production and a strong association exists among gut microbes, strawberry-derived microbial metabolites, and the vascular benefits of dietary strawberries. Further, our study provides significant proof of concept to warrant future research on the use of strawberries as a nutritional strategy to prevent vascular complications.https://www.tandfonline.com/doi/10.1080/19490976.2024.2446375Diet-derived metabolitesphytochemicalsgut microbiomevascularstrawberrieshost-microbiome interaction |
| spellingShingle | Chrissa Petersen Adhini Kuppuswamy Satheesh Babu Ceres Mattos Della Lucia Henry A. Paz Lisard Iglesias-Carres Ying Zhong Thunder Jalili J David Symons Kartik Shankar Andrew P. Neilson Umesh D. Wankhade Pon Velayutham Anandh Babu Gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammation Gut Microbes Diet-derived metabolites phytochemicals gut microbiome vascular strawberries host-microbiome interaction |
| title | Gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammation |
| title_full | Gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammation |
| title_fullStr | Gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammation |
| title_full_unstemmed | Gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammation |
| title_short | Gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammation |
| title_sort | gut microbes metabolize strawberry phytochemicals and mediate their beneficial effects on vascular inflammation |
| topic | Diet-derived metabolites phytochemicals gut microbiome vascular strawberries host-microbiome interaction |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2024.2446375 |
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