Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches
Abstract Xanthomonas oryzae pv. oryzae (Xoo) is a bacterial pathogen responsible for bacterial leaf blight (BLB) in rice, which can result in significant yield losses of up to 70%. A study evaluated the spread of Xoo in rice fields using environmental samples and employed colorimetric loop-mediated...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-85422-3 |
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| author | Onchira Ritbamrung Phithak Inthima Kumrop Ratanasut Kawee Sujipuli Tepsuda Rungrat Kittisak Buddhachat |
| author_facet | Onchira Ritbamrung Phithak Inthima Kumrop Ratanasut Kawee Sujipuli Tepsuda Rungrat Kittisak Buddhachat |
| author_sort | Onchira Ritbamrung |
| collection | DOAJ |
| description | Abstract Xanthomonas oryzae pv. oryzae (Xoo) is a bacterial pathogen responsible for bacterial leaf blight (BLB) in rice, which can result in significant yield losses of up to 70%. A study evaluated the spread of Xoo in rice fields using environmental samples and employed colorimetric loop-mediated amplification (cLAMP) and PCR for detection. Quantitative PCR (qPCR) was used to measure infection levels. The research compared infection severity between a susceptible rice variety, Phitsanulok 2 (PSL2), and a resistant variety, PSL2-Xa21. Results showed that Xoo infection decreased from the leaves to the roots, but the bacteria persisted in soil and water for up to 12 and 6 weeks, respectively. The cLAMP assay, with the LpXoo4009 primer, effectively detected Xoo at low concentrations in both soil and water. Additionally, common grasses found in rice fields, such as Eriochloa procera, Echinochloa crus-galli and Chloris barbata were identified as temporary reservoirs for Xoo, facilitating its spread. The Xoo pathogen is distributed from infected leaves to roots and then from roots to the soil and nearby water. Grasses in the fields contribute to the perpetuation of the infection cycle serving as potential reservoirs that maintain the pathogen’s presence in the environment. |
| format | Article |
| id | doaj-art-224b1b949969488ba3703093096af8f8 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-224b1b949969488ba3703093096af8f82025-01-12T12:21:41ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-025-85422-3Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approachesOnchira Ritbamrung0Phithak Inthima1Kumrop Ratanasut2Kawee Sujipuli3Tepsuda Rungrat4Kittisak Buddhachat5Department of Biology, Faculty of Science, Naresuan UniversityDepartment of Biology, Faculty of Science, Naresuan UniversityCenter of Excellence in Research for Agricultural Biotechnology, Naresuan UniversityCenter of Excellence in Research for Agricultural Biotechnology, Naresuan UniversityCenter of Excellence in Research for Agricultural Biotechnology, Naresuan UniversityDepartment of Biology, Faculty of Science, Naresuan UniversityAbstract Xanthomonas oryzae pv. oryzae (Xoo) is a bacterial pathogen responsible for bacterial leaf blight (BLB) in rice, which can result in significant yield losses of up to 70%. A study evaluated the spread of Xoo in rice fields using environmental samples and employed colorimetric loop-mediated amplification (cLAMP) and PCR for detection. Quantitative PCR (qPCR) was used to measure infection levels. The research compared infection severity between a susceptible rice variety, Phitsanulok 2 (PSL2), and a resistant variety, PSL2-Xa21. Results showed that Xoo infection decreased from the leaves to the roots, but the bacteria persisted in soil and water for up to 12 and 6 weeks, respectively. The cLAMP assay, with the LpXoo4009 primer, effectively detected Xoo at low concentrations in both soil and water. Additionally, common grasses found in rice fields, such as Eriochloa procera, Echinochloa crus-galli and Chloris barbata were identified as temporary reservoirs for Xoo, facilitating its spread. The Xoo pathogen is distributed from infected leaves to roots and then from roots to the soil and nearby water. Grasses in the fields contribute to the perpetuation of the infection cycle serving as potential reservoirs that maintain the pathogen’s presence in the environment.https://doi.org/10.1038/s41598-025-85422-3Bacterial leaf blight (BLB)XooEnvironmental DNA (eDNA)Colorimetric loop-mediated isothermal amplification (cLAMP)Grass |
| spellingShingle | Onchira Ritbamrung Phithak Inthima Kumrop Ratanasut Kawee Sujipuli Tepsuda Rungrat Kittisak Buddhachat Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches Scientific Reports Bacterial leaf blight (BLB) Xoo Environmental DNA (eDNA) Colorimetric loop-mediated isothermal amplification (cLAMP) Grass |
| title | Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches |
| title_full | Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches |
| title_fullStr | Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches |
| title_full_unstemmed | Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches |
| title_short | Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches |
| title_sort | evaluating xanthomonas oryzae pv oryzae xoo infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches |
| topic | Bacterial leaf blight (BLB) Xoo Environmental DNA (eDNA) Colorimetric loop-mediated isothermal amplification (cLAMP) Grass |
| url | https://doi.org/10.1038/s41598-025-85422-3 |
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