Minimal Inhibitory Concentration Assay of Tridax procumbens Stem Extract-Based Chitosan Gel against Pseudomonas aeruginosa
Introduction: Tridax procumbens has long been used as an insect deterrent, anticoagulant, and for the healing of wounds. In some areas of India, traditional healers also employ T. procumbens to treat injuries, blisters, and boils. A widespread rod-shaped, gram-negative, strictly aerobic bacteria cal...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_1391_24 |
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| author | Sachin Aditya M. Jeevitha Kaarthikeyan Gurumoorthy Rajeshkumar Shanmugham |
| author_facet | Sachin Aditya M. Jeevitha Kaarthikeyan Gurumoorthy Rajeshkumar Shanmugham |
| author_sort | Sachin Aditya |
| collection | DOAJ |
| description | Introduction:
Tridax procumbens has long been used as an insect deterrent, anticoagulant, and for the healing of wounds. In some areas of India, traditional healers also employ T. procumbens to treat injuries, blisters, and boils. A widespread rod-shaped, gram-negative, strictly aerobic bacteria called Pseudomonas aeruginosa can infect both plants and animals, including humans. It can be found in a variety of infections, particularly those that have a tendency to become chronic such as gingivitis and periodontitis. This study aims to assess the minimum inhibitory concentration (MIC) assay of T. procumbens stem extract-based chitosan gel against P. aeruginosa.
Materials and Methods:
The extract was produced from powdered T. procumbens stem part. To create the wound-healing gel, medium molecular weight chitosan was added to the T. procumbens stem extract to form a homogenous gel. Time-kill assay was conducted to determine the time-kill kinetics of the T. procumbens stem extract-based chitosan gel against P. aeruginosa.
Results:
The absorbance values decreased progressively over time at all concentrations (25 μL, 50 μL, and 100 μL) showing its inhibitory effect. With the increase in concentration, the inhibitory effect of T. procumbens stem extract-based chitosan gel against P. aeruginosa also increased.
Conclusion:
T. procumbens stem extract-based chitosan gel has revealed potent antimicrobial activity against P. aeruginosa and therefore the gel can be applied for efficient wound healing. |
| format | Article |
| id | doaj-art-221aeed521514ccc84144ef9868fe420 |
| institution | Kabale University |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-221aeed521514ccc84144ef9868fe4202025-01-13T10:59:39ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062024-12-0116Suppl 4S4052S405510.4103/jpbs.jpbs_1391_24Minimal Inhibitory Concentration Assay of Tridax procumbens Stem Extract-Based Chitosan Gel against Pseudomonas aeruginosaSachin AdityaM. JeevithaKaarthikeyan GurumoorthyRajeshkumar ShanmughamIntroduction: Tridax procumbens has long been used as an insect deterrent, anticoagulant, and for the healing of wounds. In some areas of India, traditional healers also employ T. procumbens to treat injuries, blisters, and boils. A widespread rod-shaped, gram-negative, strictly aerobic bacteria called Pseudomonas aeruginosa can infect both plants and animals, including humans. It can be found in a variety of infections, particularly those that have a tendency to become chronic such as gingivitis and periodontitis. This study aims to assess the minimum inhibitory concentration (MIC) assay of T. procumbens stem extract-based chitosan gel against P. aeruginosa. Materials and Methods: The extract was produced from powdered T. procumbens stem part. To create the wound-healing gel, medium molecular weight chitosan was added to the T. procumbens stem extract to form a homogenous gel. Time-kill assay was conducted to determine the time-kill kinetics of the T. procumbens stem extract-based chitosan gel against P. aeruginosa. Results: The absorbance values decreased progressively over time at all concentrations (25 μL, 50 μL, and 100 μL) showing its inhibitory effect. With the increase in concentration, the inhibitory effect of T. procumbens stem extract-based chitosan gel against P. aeruginosa also increased. Conclusion: T. procumbens stem extract-based chitosan gel has revealed potent antimicrobial activity against P. aeruginosa and therefore the gel can be applied for efficient wound healing.https://journals.lww.com/10.4103/jpbs.jpbs_1391_24antimicrobial activitychitosan geldiseaseinnovationminimal inhibitory concentrationt. procumbens |
| spellingShingle | Sachin Aditya M. Jeevitha Kaarthikeyan Gurumoorthy Rajeshkumar Shanmugham Minimal Inhibitory Concentration Assay of Tridax procumbens Stem Extract-Based Chitosan Gel against Pseudomonas aeruginosa Journal of Pharmacy and Bioallied Sciences antimicrobial activity chitosan gel disease innovation minimal inhibitory concentration t. procumbens |
| title | Minimal Inhibitory Concentration Assay of Tridax procumbens Stem Extract-Based Chitosan Gel against Pseudomonas aeruginosa |
| title_full | Minimal Inhibitory Concentration Assay of Tridax procumbens Stem Extract-Based Chitosan Gel against Pseudomonas aeruginosa |
| title_fullStr | Minimal Inhibitory Concentration Assay of Tridax procumbens Stem Extract-Based Chitosan Gel against Pseudomonas aeruginosa |
| title_full_unstemmed | Minimal Inhibitory Concentration Assay of Tridax procumbens Stem Extract-Based Chitosan Gel against Pseudomonas aeruginosa |
| title_short | Minimal Inhibitory Concentration Assay of Tridax procumbens Stem Extract-Based Chitosan Gel against Pseudomonas aeruginosa |
| title_sort | minimal inhibitory concentration assay of tridax procumbens stem extract based chitosan gel against pseudomonas aeruginosa |
| topic | antimicrobial activity chitosan gel disease innovation minimal inhibitory concentration t. procumbens |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_1391_24 |
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