A PCR-independent, annealing-free cloning method for the insertion of short DNA fragments
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| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
China Science Publishing & Media Ltd.
2024-11-01
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| Series: | Acta Biochimica et Biophysica Sinica |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2024088 |
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| _version_ | 1841525911368761344 |
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| author | Li Linbo Yan Jin Qi Yuan Xiang Zhenglong Jiang Na Yuan Tongkang Wang Zhenyi Wang Yuan Zhan Huaizhe Liu Shiyi Zhao Li Xu Jing Lei Xiaowei Liu Yuxuan Wang Gui Xie Jiayang Guo Zhenming Cai Chunhai Bian Shan |
| author_facet | Li Linbo Yan Jin Qi Yuan Xiang Zhenglong Jiang Na Yuan Tongkang Wang Zhenyi Wang Yuan Zhan Huaizhe Liu Shiyi Zhao Li Xu Jing Lei Xiaowei Liu Yuxuan Wang Gui Xie Jiayang Guo Zhenming Cai Chunhai Bian Shan |
| author_sort | Li Linbo |
| collection | DOAJ |
| format | Article |
| id | doaj-art-21fc32afc91644b9b1c919b15a24c867 |
| institution | Kabale University |
| issn | 1672-9145 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-21fc32afc91644b9b1c919b15a24c8672025-01-17T05:58:24ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452024-11-01561886189010.3724/abbs.202408820d259ccA PCR-independent, annealing-free cloning method for the insertion of short DNA fragmentsLi Linbo0Yan Jin1Qi Yuan2Xiang Zhenglong3Jiang Na4Yuan Tongkang5Wang Zhenyi6Wang Yuan7Zhan Huaizhe8Liu Shiyi9Zhao Li10Xu Jing11Lei Xiaowei12Liu Yuxuan13Wang Gui14Xie Jiayang15Guo Zhenming16Cai Chunhai17Bian Shan18["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Department of Biology, Brandeis University, Waltham, MA 02453, USA"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China"]["Institute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China","National Health Commission Key Laboratory of Birth Defect Research and Prevention, Hunan Provincial Maternal and Child Health Care Hospital, University of South China, Changsha 410008, China","China Regional Research Centre, International Centre for Genetic Engineering and Biotechnology, Taizhou 212200, China"]https://www.sciengine.com/doi/10.3724/abbs.2024088 |
| spellingShingle | Li Linbo Yan Jin Qi Yuan Xiang Zhenglong Jiang Na Yuan Tongkang Wang Zhenyi Wang Yuan Zhan Huaizhe Liu Shiyi Zhao Li Xu Jing Lei Xiaowei Liu Yuxuan Wang Gui Xie Jiayang Guo Zhenming Cai Chunhai Bian Shan A PCR-independent, annealing-free cloning method for the insertion of short DNA fragments Acta Biochimica et Biophysica Sinica |
| title | A PCR-independent, annealing-free cloning method for the insertion of short DNA fragments |
| title_full | A PCR-independent, annealing-free cloning method for the insertion of short DNA fragments |
| title_fullStr | A PCR-independent, annealing-free cloning method for the insertion of short DNA fragments |
| title_full_unstemmed | A PCR-independent, annealing-free cloning method for the insertion of short DNA fragments |
| title_short | A PCR-independent, annealing-free cloning method for the insertion of short DNA fragments |
| title_sort | pcr independent annealing free cloning method for the insertion of short dna fragments |
| url | https://www.sciengine.com/doi/10.3724/abbs.2024088 |
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