Mesenchymal Stem/Stromal Cells Reverse Adipose Tissue Inflammation in Pigs with Metabolic Syndrome and Renovascular Hypertension
Metabolic syndrome (MetS) is associated with low-grade inflammation, which can be exacerbated by renal artery stenosis (RAS) and renovascular hypertension, potentially worsening outcomes through pro-inflammatory cytokines. This study investigated whether mesenchymal stem/stromal cells (MSCs) could r...
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2025-01-01
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| author | Alexander B. C. Krueger Xiangyang Zhu Sarosh Siddiqi Emma C. Whitehead Hui Tang Kyra L. Jordan Amir Lerman Lilach O. Lerman |
| author_facet | Alexander B. C. Krueger Xiangyang Zhu Sarosh Siddiqi Emma C. Whitehead Hui Tang Kyra L. Jordan Amir Lerman Lilach O. Lerman |
| author_sort | Alexander B. C. Krueger |
| collection | DOAJ |
| description | Metabolic syndrome (MetS) is associated with low-grade inflammation, which can be exacerbated by renal artery stenosis (RAS) and renovascular hypertension, potentially worsening outcomes through pro-inflammatory cytokines. This study investigated whether mesenchymal stem/stromal cells (MSCs) could reduce fat inflammation in pigs with MetS and RAS. Twenty-four pigs were divided into Lean (control), MetS, MetS + RAS, and MetS + RAS + MSCs. In the MSC-treated group, autologous adipose-derived MSCs (10<sup>7</sup> cells) were injected into the renal artery six weeks after RAS induction. After four weeks, fat volumes and inflammatory markers were assessed. MSC treatment reduced levels of pro-inflammatory cytokines (MCP-1, TNF-a, IL-6) in the renal vein blood and in perirenal fat. The MSCs also decreased fat fibrosis, restored adipocyte size, and altered adipogenesis-related gene expression, particularly in the perirenal fat. These effects were less pronounced in subcutaneous fat. The MSC therapy attenuated fat inflammation and improved metabolic outcomes in pigs with MetS + RAS, suggesting that adipose-derived MSCs may offer a promising therapeutic approach for metabolic disorders. |
| format | Article |
| id | doaj-art-21c4ef6136f846b58798030706dd7dda |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-21c4ef6136f846b58798030706dd7dda2025-01-10T13:16:20ZengMDPI AGCells2073-44092025-01-011414010.3390/cells14010040Mesenchymal Stem/Stromal Cells Reverse Adipose Tissue Inflammation in Pigs with Metabolic Syndrome and Renovascular HypertensionAlexander B. C. Krueger0Xiangyang Zhu1Sarosh Siddiqi2Emma C. Whitehead3Hui Tang4Kyra L. Jordan5Amir Lerman6Lilach O. Lerman7Division of Nephrology & Hypertension, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USADivision of Nephrology & Hypertension, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USADivision of Nephrology & Hypertension, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USADivision of Nephrology & Hypertension, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USADivision of Nephrology & Hypertension, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USADivision of Nephrology & Hypertension, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USADepartment of Cardiovascular Diseases, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USADivision of Nephrology & Hypertension, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USAMetabolic syndrome (MetS) is associated with low-grade inflammation, which can be exacerbated by renal artery stenosis (RAS) and renovascular hypertension, potentially worsening outcomes through pro-inflammatory cytokines. This study investigated whether mesenchymal stem/stromal cells (MSCs) could reduce fat inflammation in pigs with MetS and RAS. Twenty-four pigs were divided into Lean (control), MetS, MetS + RAS, and MetS + RAS + MSCs. In the MSC-treated group, autologous adipose-derived MSCs (10<sup>7</sup> cells) were injected into the renal artery six weeks after RAS induction. After four weeks, fat volumes and inflammatory markers were assessed. MSC treatment reduced levels of pro-inflammatory cytokines (MCP-1, TNF-a, IL-6) in the renal vein blood and in perirenal fat. The MSCs also decreased fat fibrosis, restored adipocyte size, and altered adipogenesis-related gene expression, particularly in the perirenal fat. These effects were less pronounced in subcutaneous fat. The MSC therapy attenuated fat inflammation and improved metabolic outcomes in pigs with MetS + RAS, suggesting that adipose-derived MSCs may offer a promising therapeutic approach for metabolic disorders.https://www.mdpi.com/2073-4409/14/1/40metabolic syndromerenal artery stenosismesenchymal stem cellsinflammation |
| spellingShingle | Alexander B. C. Krueger Xiangyang Zhu Sarosh Siddiqi Emma C. Whitehead Hui Tang Kyra L. Jordan Amir Lerman Lilach O. Lerman Mesenchymal Stem/Stromal Cells Reverse Adipose Tissue Inflammation in Pigs with Metabolic Syndrome and Renovascular Hypertension Cells metabolic syndrome renal artery stenosis mesenchymal stem cells inflammation |
| title | Mesenchymal Stem/Stromal Cells Reverse Adipose Tissue Inflammation in Pigs with Metabolic Syndrome and Renovascular Hypertension |
| title_full | Mesenchymal Stem/Stromal Cells Reverse Adipose Tissue Inflammation in Pigs with Metabolic Syndrome and Renovascular Hypertension |
| title_fullStr | Mesenchymal Stem/Stromal Cells Reverse Adipose Tissue Inflammation in Pigs with Metabolic Syndrome and Renovascular Hypertension |
| title_full_unstemmed | Mesenchymal Stem/Stromal Cells Reverse Adipose Tissue Inflammation in Pigs with Metabolic Syndrome and Renovascular Hypertension |
| title_short | Mesenchymal Stem/Stromal Cells Reverse Adipose Tissue Inflammation in Pigs with Metabolic Syndrome and Renovascular Hypertension |
| title_sort | mesenchymal stem stromal cells reverse adipose tissue inflammation in pigs with metabolic syndrome and renovascular hypertension |
| topic | metabolic syndrome renal artery stenosis mesenchymal stem cells inflammation |
| url | https://www.mdpi.com/2073-4409/14/1/40 |
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