Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms
Very small embryonic-like stem cells (VSELs) represent a unique rare population of adult stem cells (SCs) sharing several structural, genetic, biochemical, and functional properties with embryonic SCs and have been identified in several adult murine and human tissues. However, rat bone marrow- (BM-)...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/5069857 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841524604728770560 |
|---|---|
| author | Anna Labedz-Maslowska Elzbieta Kamycka Sylwia Bobis-Wozowicz Zbigniew Madeja Ewa K. Zuba-Surma |
| author_facet | Anna Labedz-Maslowska Elzbieta Kamycka Sylwia Bobis-Wozowicz Zbigniew Madeja Ewa K. Zuba-Surma |
| author_sort | Anna Labedz-Maslowska |
| collection | DOAJ |
| description | Very small embryonic-like stem cells (VSELs) represent a unique rare population of adult stem cells (SCs) sharing several structural, genetic, biochemical, and functional properties with embryonic SCs and have been identified in several adult murine and human tissues. However, rat bone marrow- (BM-) derived SCs closely resembling murine or human VSELs have not been described. Thus, we employed multi-instrumental flow cytometric approach including classical and imaging cytometry and we established that newly identified population of nonhematopoietic cells expressing CD106 (VCAM-I) antigen contains SCs with very small size, expressing markers of pluripotency (Oct-4A and Nanog) on both mRNA and protein levels that indicate VSEL population. Based on our experience in both murine and human VSEL isolation procedures by fluorescence-activated cell sorting (FACS), we also optimized sorting protocol for separation of CD45−/Lin−/CD106+ rat BM-derived VSELs from wild type and eGFP-expressing rats, which are often used as donor animals for cell transplantations in regenerative studies in vivo. Thus, this is a first study identifying multiantigenic phenotype and providing sorting protocols for isolation VSELs from rat BM tissue for further examining of their functional properties in vitro as well as regenerative capacity in distinct in vivo rat models of tissue injury. |
| format | Article |
| id | doaj-art-21bba47738a943dabd1db4b58f781698 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-21bba47738a943dabd1db4b58f7816982025-02-03T05:47:41ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/50698575069857Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric PlatformsAnna Labedz-Maslowska0Elzbieta Kamycka1Sylwia Bobis-Wozowicz2Zbigniew Madeja3Ewa K. Zuba-Surma4Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, PolandDepartment of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, PolandDepartment of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, PolandDepartment of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, PolandDepartment of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, PolandVery small embryonic-like stem cells (VSELs) represent a unique rare population of adult stem cells (SCs) sharing several structural, genetic, biochemical, and functional properties with embryonic SCs and have been identified in several adult murine and human tissues. However, rat bone marrow- (BM-) derived SCs closely resembling murine or human VSELs have not been described. Thus, we employed multi-instrumental flow cytometric approach including classical and imaging cytometry and we established that newly identified population of nonhematopoietic cells expressing CD106 (VCAM-I) antigen contains SCs with very small size, expressing markers of pluripotency (Oct-4A and Nanog) on both mRNA and protein levels that indicate VSEL population. Based on our experience in both murine and human VSEL isolation procedures by fluorescence-activated cell sorting (FACS), we also optimized sorting protocol for separation of CD45−/Lin−/CD106+ rat BM-derived VSELs from wild type and eGFP-expressing rats, which are often used as donor animals for cell transplantations in regenerative studies in vivo. Thus, this is a first study identifying multiantigenic phenotype and providing sorting protocols for isolation VSELs from rat BM tissue for further examining of their functional properties in vitro as well as regenerative capacity in distinct in vivo rat models of tissue injury.http://dx.doi.org/10.1155/2016/5069857 |
| spellingShingle | Anna Labedz-Maslowska Elzbieta Kamycka Sylwia Bobis-Wozowicz Zbigniew Madeja Ewa K. Zuba-Surma Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms Stem Cells International |
| title | Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms |
| title_full | Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms |
| title_fullStr | Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms |
| title_full_unstemmed | Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms |
| title_short | Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms |
| title_sort | identification of new rat bone marrow derived population of very small stem cell with oct 4a and nanog expression by flow cytometric platforms |
| url | http://dx.doi.org/10.1155/2016/5069857 |
| work_keys_str_mv | AT annalabedzmaslowska identificationofnewratbonemarrowderivedpopulationofverysmallstemcellwithoct4aandnanogexpressionbyflowcytometricplatforms AT elzbietakamycka identificationofnewratbonemarrowderivedpopulationofverysmallstemcellwithoct4aandnanogexpressionbyflowcytometricplatforms AT sylwiabobiswozowicz identificationofnewratbonemarrowderivedpopulationofverysmallstemcellwithoct4aandnanogexpressionbyflowcytometricplatforms AT zbigniewmadeja identificationofnewratbonemarrowderivedpopulationofverysmallstemcellwithoct4aandnanogexpressionbyflowcytometricplatforms AT ewakzubasurma identificationofnewratbonemarrowderivedpopulationofverysmallstemcellwithoct4aandnanogexpressionbyflowcytometricplatforms |