Genome-Wide Identification and Expression Analysis of the <i>ALDH</i> Gene Family in <i>Sinonovacula constricta</i> Bivalve in Response to Acute Hypersaline Stress
The razor clam <i>Sinonovacula constricta</i>, a significant marine bivalve species, inhabits estuaries and encounters salinity stress. Despite its commercial importance, there is limited understanding of its adaptive mechanisms to high salinity. Aldehyde dehydrogenases (ALDHs), which be...
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author | Jianing Yu Biao Wu Yinghui Dong Zhihua Lin Hanhan Yao |
author_facet | Jianing Yu Biao Wu Yinghui Dong Zhihua Lin Hanhan Yao |
author_sort | Jianing Yu |
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description | The razor clam <i>Sinonovacula constricta</i>, a significant marine bivalve species, inhabits estuaries and encounters salinity stress. Despite its commercial importance, there is limited understanding of its adaptive mechanisms to high salinity. Aldehyde dehydrogenases (ALDHs), which belong to the NAD(P)<sup>+</sup>-dependent superfamily, play a crucial role in stress resilience by participating in catabolic and anabolic pathways, such as carnitine synthesis, glycolysis, and amino acid metabolism. This study presents the first comprehensive analysis of the ALDH family in <i>S. constricta</i> under acute high salt stress conditions and identifies 16 <i>ScALDH</i> genes across 10 subfamilies. These genes are located on eight chromosomes, with tandem duplications observed on chromosome 10; they encode mostly acidic and hydrophilic proteins. Among them, <i>ScALDH18A1</i> contains a conserved P5CS domain that is implicated in proline synthesis and osmotic regulation. The expression of 14 <i>ScALDH</i> members were significantly altered under acute salt stress conditions, with <i>ScALDH8</i> and <i>ScALDH18A1</i> showing increased expression levels, suggesting their involvement in osmotic pressure regulation. This research provides insights into the characteristics, evolution, and response to salinity stress of the <i>ScALDH</i> gene family while shedding light on ALDH function in bivalves, as well as serving as a foundation for further studies on osmotic stress regulation. |
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spelling | doaj-art-217c78d7cfd4431f99210bd7d610c34a2025-01-10T13:13:57ZengMDPI AGAnimals2076-26152024-12-011516410.3390/ani15010064Genome-Wide Identification and Expression Analysis of the <i>ALDH</i> Gene Family in <i>Sinonovacula constricta</i> Bivalve in Response to Acute Hypersaline StressJianing Yu0Biao Wu1Yinghui Dong2Zhihua Lin3Hanhan Yao4College of Advanced Agricultural Sciences, Zhejiang Wanli University, Ningbo 315101, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaCollege of Advanced Agricultural Sciences, Zhejiang Wanli University, Ningbo 315101, ChinaCollege of Advanced Agricultural Sciences, Zhejiang Wanli University, Ningbo 315101, ChinaCollege of Advanced Agricultural Sciences, Zhejiang Wanli University, Ningbo 315101, ChinaThe razor clam <i>Sinonovacula constricta</i>, a significant marine bivalve species, inhabits estuaries and encounters salinity stress. Despite its commercial importance, there is limited understanding of its adaptive mechanisms to high salinity. Aldehyde dehydrogenases (ALDHs), which belong to the NAD(P)<sup>+</sup>-dependent superfamily, play a crucial role in stress resilience by participating in catabolic and anabolic pathways, such as carnitine synthesis, glycolysis, and amino acid metabolism. This study presents the first comprehensive analysis of the ALDH family in <i>S. constricta</i> under acute high salt stress conditions and identifies 16 <i>ScALDH</i> genes across 10 subfamilies. These genes are located on eight chromosomes, with tandem duplications observed on chromosome 10; they encode mostly acidic and hydrophilic proteins. Among them, <i>ScALDH18A1</i> contains a conserved P5CS domain that is implicated in proline synthesis and osmotic regulation. The expression of 14 <i>ScALDH</i> members were significantly altered under acute salt stress conditions, with <i>ScALDH8</i> and <i>ScALDH18A1</i> showing increased expression levels, suggesting their involvement in osmotic pressure regulation. This research provides insights into the characteristics, evolution, and response to salinity stress of the <i>ScALDH</i> gene family while shedding light on ALDH function in bivalves, as well as serving as a foundation for further studies on osmotic stress regulation.https://www.mdpi.com/2076-2615/15/1/64<i>Sinonovacula constricta</i>ALDH gene familyosmotic pressure regulationaldehyde dehydrogenaseoxidative stressproline |
spellingShingle | Jianing Yu Biao Wu Yinghui Dong Zhihua Lin Hanhan Yao Genome-Wide Identification and Expression Analysis of the <i>ALDH</i> Gene Family in <i>Sinonovacula constricta</i> Bivalve in Response to Acute Hypersaline Stress Animals <i>Sinonovacula constricta</i> ALDH gene family osmotic pressure regulation aldehyde dehydrogenase oxidative stress proline |
title | Genome-Wide Identification and Expression Analysis of the <i>ALDH</i> Gene Family in <i>Sinonovacula constricta</i> Bivalve in Response to Acute Hypersaline Stress |
title_full | Genome-Wide Identification and Expression Analysis of the <i>ALDH</i> Gene Family in <i>Sinonovacula constricta</i> Bivalve in Response to Acute Hypersaline Stress |
title_fullStr | Genome-Wide Identification and Expression Analysis of the <i>ALDH</i> Gene Family in <i>Sinonovacula constricta</i> Bivalve in Response to Acute Hypersaline Stress |
title_full_unstemmed | Genome-Wide Identification and Expression Analysis of the <i>ALDH</i> Gene Family in <i>Sinonovacula constricta</i> Bivalve in Response to Acute Hypersaline Stress |
title_short | Genome-Wide Identification and Expression Analysis of the <i>ALDH</i> Gene Family in <i>Sinonovacula constricta</i> Bivalve in Response to Acute Hypersaline Stress |
title_sort | genome wide identification and expression analysis of the i aldh i gene family in i sinonovacula constricta i bivalve in response to acute hypersaline stress |
topic | <i>Sinonovacula constricta</i> ALDH gene family osmotic pressure regulation aldehyde dehydrogenase oxidative stress proline |
url | https://www.mdpi.com/2076-2615/15/1/64 |
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