Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model
Dysbiosis, marked by a decline in Firmicutes (such as Clostridium spp.) and a rise in Proteobacteria (such as Escherichia coli), is recognized as a pathway linked to the progression of non-alcoholic fatty liver disease (NAFLD). High-fat food may influence the development of NAFLD. This study investi...
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University of Brawijaya
2024-06-01
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| Series: | Journal of Tropical Life Science |
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| Online Access: | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2840 |
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| author | Syifa Mustika Nabila Ramadhani Novita Apramadha Kartika Sari Dian Handayani Dewi Santosaningsih |
| author_facet | Syifa Mustika Nabila Ramadhani Novita Apramadha Kartika Sari Dian Handayani Dewi Santosaningsih |
| author_sort | Syifa Mustika |
| collection | DOAJ |
| description | Dysbiosis, marked by a decline in Firmicutes (such as Clostridium spp.) and a rise in Proteobacteria (such as Escherichia coli), is recognized as a pathway linked to the progression of non-alcoholic fatty liver disease (NAFLD). High-fat food may influence the development of NAFLD. This study investigates the effect of different high-fat diets on gut microbiota composition and butyrate levels in a rat model. Four groups of Rattus norvegicus strain Wistar (n=27) were fed specific diets for 12 weeks: normal diet (ND, n=7), high-fat diet (HFD, n=6), western diet (WD, n=7), and high-fat-high-fructose diet (HFHFD, n=7). Stools were cultured on Eosin Methylene Blue (EMB) for Escherichia coli and Forget Fredette Agar (FFA) for Clostridium spp. Colonies were counted using Total Plate Count (TPC), and butyrate levels were measured with gas chromatography. Liver histology was assessed with the NAFLD activity score (NAS). Gut microbiota were analyzed using Crosstab and Chi-Square tests, and butyrate levels with the Kruskal-Wallis test. Butyrate levels decreased in NAFLD-inducing diet groups (HFD, WD, HFHFD) compared to ND (p=0.021). Escherichia coli colony counts were significantly higher in NAFLD-inducing diet groups (p=0.048), while Clostridium spp. counts were higher in ND (p=0.001). Liver histological changes, particularly in the WD group, resembled NAFLD. These findings imply that different high-fat diets can alter the gut microbiome, potentially influencing NAFLD development. |
| format | Article |
| id | doaj-art-2138c3c4d0a148fa802d407ac1f4c062 |
| institution | Kabale University |
| issn | 2087-5517 2527-4376 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj-art-2138c3c4d0a148fa802d407ac1f4c0622024-12-03T01:19:28ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172527-43762024-06-01142277 – 284277 – 28410.11594/jtls.14.02.082785Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat ModelSyifa Mustika0Nabila Ramadhani1Novita Apramadha Kartika Sari2Dian Handayani3Dewi Santosaningsih4Doctoral Program of Medical Science, Faculty of Medicine, Universitas BrawijayaFaculty of Medicine, Universitas BrawijayaFaculty of Medicine, Universitas BrawijayaDepartment of Nutrition, Faculty of Health Science, Universitas BrawijayaDepartment of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya - dr. Saiful Anwar Hospital, MalangDysbiosis, marked by a decline in Firmicutes (such as Clostridium spp.) and a rise in Proteobacteria (such as Escherichia coli), is recognized as a pathway linked to the progression of non-alcoholic fatty liver disease (NAFLD). High-fat food may influence the development of NAFLD. This study investigates the effect of different high-fat diets on gut microbiota composition and butyrate levels in a rat model. Four groups of Rattus norvegicus strain Wistar (n=27) were fed specific diets for 12 weeks: normal diet (ND, n=7), high-fat diet (HFD, n=6), western diet (WD, n=7), and high-fat-high-fructose diet (HFHFD, n=7). Stools were cultured on Eosin Methylene Blue (EMB) for Escherichia coli and Forget Fredette Agar (FFA) for Clostridium spp. Colonies were counted using Total Plate Count (TPC), and butyrate levels were measured with gas chromatography. Liver histology was assessed with the NAFLD activity score (NAS). Gut microbiota were analyzed using Crosstab and Chi-Square tests, and butyrate levels with the Kruskal-Wallis test. Butyrate levels decreased in NAFLD-inducing diet groups (HFD, WD, HFHFD) compared to ND (p=0.021). Escherichia coli colony counts were significantly higher in NAFLD-inducing diet groups (p=0.048), while Clostridium spp. counts were higher in ND (p=0.001). Liver histological changes, particularly in the WD group, resembled NAFLD. These findings imply that different high-fat diets can alter the gut microbiome, potentially influencing NAFLD development.https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2840butyrate clostridium spp.dietescherichia colinafld |
| spellingShingle | Syifa Mustika Nabila Ramadhani Novita Apramadha Kartika Sari Dian Handayani Dewi Santosaningsih Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model Journal of Tropical Life Science butyrate clostridium spp. diet escherichia coli nafld |
| title | Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model |
| title_full | Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model |
| title_fullStr | Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model |
| title_full_unstemmed | Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model |
| title_short | Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model |
| title_sort | effect of multiple types of high fat diet induction on gut microbiota profile and butyrate levels on nafld rat model |
| topic | butyrate clostridium spp. diet escherichia coli nafld |
| url | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2840 |
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