Development and characterization of a low intensity vibrational system for microgravity studies

Abstract Extended-duration human spaceflight necessitates a better understanding of the physiological impacts of microgravity. While the ground-based microgravity simulations identified low intensity vibration (LIV) as a possible countermeasure, how cells may respond to LIV under real microgravity r...

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Main Authors: Omor M. Khan, Will Gasperini, Chess Necessary, Zach Jacobs, Sam Perry, Jason Rexroat, Kendall Nelson, Paul Gamble, Twyman Clements, Maximilien DeLeon, Sean Howard, Anamaria Zavala, Mary Farach-Carson, Elizabeth Blaber, Danielle Wu, Aykut Satici, Gunes Uzer
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:npj Microgravity
Online Access:https://doi.org/10.1038/s41526-024-00444-x
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author Omor M. Khan
Will Gasperini
Chess Necessary
Zach Jacobs
Sam Perry
Jason Rexroat
Kendall Nelson
Paul Gamble
Twyman Clements
Maximilien DeLeon
Sean Howard
Anamaria Zavala
Mary Farach-Carson
Elizabeth Blaber
Danielle Wu
Aykut Satici
Gunes Uzer
author_facet Omor M. Khan
Will Gasperini
Chess Necessary
Zach Jacobs
Sam Perry
Jason Rexroat
Kendall Nelson
Paul Gamble
Twyman Clements
Maximilien DeLeon
Sean Howard
Anamaria Zavala
Mary Farach-Carson
Elizabeth Blaber
Danielle Wu
Aykut Satici
Gunes Uzer
author_sort Omor M. Khan
collection DOAJ
description Abstract Extended-duration human spaceflight necessitates a better understanding of the physiological impacts of microgravity. While the ground-based microgravity simulations identified low intensity vibration (LIV) as a possible countermeasure, how cells may respond to LIV under real microgravity remain unexplored. In this way, adaptation of LIV bioreactors for space remains limited, resulting in a significant gap in microgravity research. In this study, we introduce an LIV bioreactor designed specifically for the usage in the International Space Station. Our research covers the bioreactor’s design process and evaluation of the short-term viability of cells encapsulated in hydrogel-laden 3D printed scaffolds under 0.7 g, 90 Hz LIV. An LIV bioreactor compatible with the operation requirements of space missions provides a robust platform to study cellular effects of LIV under real microgravity conditions.
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series npj Microgravity
spelling doaj-art-2136f14666da42ca8fbbf6b6e5c9e7ce2024-11-24T12:37:15ZengNature Portfolionpj Microgravity2373-80652024-11-011011910.1038/s41526-024-00444-xDevelopment and characterization of a low intensity vibrational system for microgravity studiesOmor M. Khan0Will Gasperini1Chess Necessary2Zach Jacobs3Sam Perry4Jason Rexroat5Kendall Nelson6Paul Gamble7Twyman Clements8Maximilien DeLeon9Sean Howard10Anamaria Zavala11Mary Farach-Carson12Elizabeth Blaber13Danielle Wu14Aykut Satici15Gunes Uzer16Department of Mechanical and Biomedical Engineering, Boise State UniversityDepartment of Mechanical and Biomedical Engineering, Boise State UniversitySpace Tango IncSpace Tango IncSpace Tango IncSpace Tango IncSpace Tango IncSpace Tango IncSpace Tango IncDepartment of Bioengineering, Rice UniversityDepartment of Mechanical and Biomedical Engineering, Boise State UniversityDepartment of Mechanical and Biomedical Engineering, Boise State UniversityDepartment of Bioengineering, Rice UniversityCenter for Biotechnology and Rd. Interdisciplinary Studies, Department of Biomedical Engineering, Rensselaer Polytechnic InstituteDepartment of Bioengineering, Rice UniversityDepartment of Mechanical and Biomedical Engineering, Boise State UniversityDepartment of Mechanical and Biomedical Engineering, Boise State UniversityAbstract Extended-duration human spaceflight necessitates a better understanding of the physiological impacts of microgravity. While the ground-based microgravity simulations identified low intensity vibration (LIV) as a possible countermeasure, how cells may respond to LIV under real microgravity remain unexplored. In this way, adaptation of LIV bioreactors for space remains limited, resulting in a significant gap in microgravity research. In this study, we introduce an LIV bioreactor designed specifically for the usage in the International Space Station. Our research covers the bioreactor’s design process and evaluation of the short-term viability of cells encapsulated in hydrogel-laden 3D printed scaffolds under 0.7 g, 90 Hz LIV. An LIV bioreactor compatible with the operation requirements of space missions provides a robust platform to study cellular effects of LIV under real microgravity conditions.https://doi.org/10.1038/s41526-024-00444-x
spellingShingle Omor M. Khan
Will Gasperini
Chess Necessary
Zach Jacobs
Sam Perry
Jason Rexroat
Kendall Nelson
Paul Gamble
Twyman Clements
Maximilien DeLeon
Sean Howard
Anamaria Zavala
Mary Farach-Carson
Elizabeth Blaber
Danielle Wu
Aykut Satici
Gunes Uzer
Development and characterization of a low intensity vibrational system for microgravity studies
npj Microgravity
title Development and characterization of a low intensity vibrational system for microgravity studies
title_full Development and characterization of a low intensity vibrational system for microgravity studies
title_fullStr Development and characterization of a low intensity vibrational system for microgravity studies
title_full_unstemmed Development and characterization of a low intensity vibrational system for microgravity studies
title_short Development and characterization of a low intensity vibrational system for microgravity studies
title_sort development and characterization of a low intensity vibrational system for microgravity studies
url https://doi.org/10.1038/s41526-024-00444-x
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