Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathways
This paper intended to study the mechanism and active ingredients of ACB anti-epilepsy. The antiepileptic activity of ACB validated in PTZ kindled rats, ACB could increase the seizure latency and reduce seizure duration, attenuate spatial learning and memory deficits, improve hippocampus neuronal da...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Taibah University for Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2023.2289239 |
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| author | Lian Zhong Chengxun He Meiyan Li Xu Wu Yan Gou Zhao Geng Chengjun He Chongbo Zhao Chunjie Wu |
| author_facet | Lian Zhong Chengxun He Meiyan Li Xu Wu Yan Gou Zhao Geng Chengjun He Chongbo Zhao Chunjie Wu |
| author_sort | Lian Zhong |
| collection | DOAJ |
| description | This paper intended to study the mechanism and active ingredients of ACB anti-epilepsy. The antiepileptic activity of ACB validated in PTZ kindled rats, ACB could increase the seizure latency and reduce seizure duration, attenuate spatial learning and memory deficits, improve hippocampus neuronal damage and regulate unbalanced neurotransmitters. Furthermore, network pharmacology and molecular docking analysis predicted four potential active compounds, in addition, PI3K/Akt signal pathway may be the main signal pathway of ACB anti-epilepsy. In vitro, ACB greatly increased the vitality and reduced apoptosis of PC12 cells exposed to H2O2. Additionally, ACB elevated Bcl-2 and downregulated C-caspase-3 and Bax proteins expression. Importantly, ACB improved the phosphorylation of PI3K and Akt in H2O2-stimulated PC12 cells, and stimulated the nuclear transfer of Nrf2. These findings indicated that ACB has effective on antiepileptic by activating of the PI3K/Akt/Nrf2 pathway to reduce oxidative stress and neuronal cell apoptosis. |
| format | Article |
| id | doaj-art-2127edcdeb5c4d97bb5bc5592d0b2bc6 |
| institution | Kabale University |
| issn | 1658-3655 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj-art-2127edcdeb5c4d97bb5bc5592d0b2bc62024-12-17T11:38:48ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552024-12-0118110.1080/16583655.2023.2289239Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathwaysLian Zhong0Chengxun He1Meiyan Li2Xu Wu3Yan Gou4Zhao Geng5Chengjun He6Chongbo Zhao7Chunjie Wu8School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, People’s Republic of ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, People’s Republic of ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, People’s Republic of ChinaCollege of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, People’s Republic of ChinaNational Medical Products Administration Key Laboratory for Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine), Sichuan Institute for Drug Control (Sichuan Testing Center of Medical Devices), Chengdu, People’s Republic of ChinaNational Medical Products Administration Key Laboratory for Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine), Sichuan Institute for Drug Control (Sichuan Testing Center of Medical Devices), Chengdu, People’s Republic of ChinaNational Medical Products Administration Key Laboratory for Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine), Sichuan Institute for Drug Control (Sichuan Testing Center of Medical Devices), Chengdu, People’s Republic of ChinaCollege of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, People’s Republic of ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, People’s Republic of ChinaThis paper intended to study the mechanism and active ingredients of ACB anti-epilepsy. The antiepileptic activity of ACB validated in PTZ kindled rats, ACB could increase the seizure latency and reduce seizure duration, attenuate spatial learning and memory deficits, improve hippocampus neuronal damage and regulate unbalanced neurotransmitters. Furthermore, network pharmacology and molecular docking analysis predicted four potential active compounds, in addition, PI3K/Akt signal pathway may be the main signal pathway of ACB anti-epilepsy. In vitro, ACB greatly increased the vitality and reduced apoptosis of PC12 cells exposed to H2O2. Additionally, ACB elevated Bcl-2 and downregulated C-caspase-3 and Bax proteins expression. Importantly, ACB improved the phosphorylation of PI3K and Akt in H2O2-stimulated PC12 cells, and stimulated the nuclear transfer of Nrf2. These findings indicated that ACB has effective on antiepileptic by activating of the PI3K/Akt/Nrf2 pathway to reduce oxidative stress and neuronal cell apoptosis.https://www.tandfonline.com/doi/10.1080/16583655.2023.2289239Arisaema Cum Bile (ACB)epilepsynetwork pharmacologyPI3K/Akt/Nrf2 signaling pathwayapoptosis |
| spellingShingle | Lian Zhong Chengxun He Meiyan Li Xu Wu Yan Gou Zhao Geng Chengjun He Chongbo Zhao Chunjie Wu Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathways Journal of Taibah University for Science Arisaema Cum Bile (ACB) epilepsy network pharmacology PI3K/Akt/Nrf2 signaling pathway apoptosis |
| title | Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathways |
| title_full | Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathways |
| title_fullStr | Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathways |
| title_full_unstemmed | Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathways |
| title_short | Arisaema Cum Bile possesses significantly antiepileptic effects through regulation of PI3K/Akt/Nrf2 signaling pathways |
| title_sort | arisaema cum bile possesses significantly antiepileptic effects through regulation of pi3k akt nrf2 signaling pathways |
| topic | Arisaema Cum Bile (ACB) epilepsy network pharmacology PI3K/Akt/Nrf2 signaling pathway apoptosis |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2023.2289239 |
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