New Approach Methodologies: Physiological responses of daphnids to pharmaceutical mixtures
Conventional methods for water monitoring are insufficient to capture the impacts of pollution because of their sensitivity limits, while they also fail to provide mechanistic insight regarding the actions of pollutants. On the other hand, New Approach Methodologies are more and more introduced as s...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-11-01
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| Series: | Journal of Hazardous Materials Letters |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666911024000388 |
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| _version_ | 1841561241358696448 |
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| author | Emma Rowan Anne Leung Katie O’Rourke Xiaofei Yin Lorraine Brennan Konstantinos Grintzalis |
| author_facet | Emma Rowan Anne Leung Katie O’Rourke Xiaofei Yin Lorraine Brennan Konstantinos Grintzalis |
| author_sort | Emma Rowan |
| collection | DOAJ |
| description | Conventional methods for water monitoring are insufficient to capture the impacts of pollution because of their sensitivity limits, while they also fail to provide mechanistic insight regarding the actions of pollutants. On the other hand, New Approach Methodologies are more and more introduced as supplementary tools that provide sensitive metrics for pollution assessment. In this context, freshwater sentinel species commonly known as water fleas were used to assess the impact of a pharmaceutical mixture. The pharmaceutical cocktail comprised of representatives of commonly encountered pharmaceuticals and specifically diclofenac, metformin, gabapentin, amoxicillin, trimethoprim, and erythromycin. Combining toxicity, phenotypic and molecular endpoints, results showed differences in mortality, feeding, key enzyme activities and metabolic perturbations, thus supporting a distinct pattern in physiological responses of daphnids that could be used to monitor and predict pollution early. |
| format | Article |
| id | doaj-art-212486d3c93f41dd9594f6c160b5fac6 |
| institution | Kabale University |
| issn | 2666-9110 |
| language | English |
| publishDate | 2025-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Hazardous Materials Letters |
| spelling | doaj-art-212486d3c93f41dd9594f6c160b5fac62025-01-03T04:09:02ZengElsevierJournal of Hazardous Materials Letters2666-91102025-11-016100139New Approach Methodologies: Physiological responses of daphnids to pharmaceutical mixturesEmma Rowan0Anne Leung1Katie O’Rourke2Xiaofei Yin3Lorraine Brennan4Konstantinos Grintzalis5School of Biotechnology, Dublin City University, Dublin D09 Y5NO, IrelandSchool of Biotechnology, Dublin City University, Dublin D09 Y5NO, IrelandSchool of Biotechnology, Dublin City University, Dublin D09 Y5NO, IrelandUCD Institute of Food and Health, UCD School of Agriculture and Food Science, University College Dublin, Dublin D04 F438, Ireland; UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin D04 F438, IrelandUCD Institute of Food and Health, UCD School of Agriculture and Food Science, University College Dublin, Dublin D04 F438, Ireland; UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin D04 F438, IrelandSchool of Biotechnology, Dublin City University, Dublin D09 Y5NO, Ireland; Corresponding author.Conventional methods for water monitoring are insufficient to capture the impacts of pollution because of their sensitivity limits, while they also fail to provide mechanistic insight regarding the actions of pollutants. On the other hand, New Approach Methodologies are more and more introduced as supplementary tools that provide sensitive metrics for pollution assessment. In this context, freshwater sentinel species commonly known as water fleas were used to assess the impact of a pharmaceutical mixture. The pharmaceutical cocktail comprised of representatives of commonly encountered pharmaceuticals and specifically diclofenac, metformin, gabapentin, amoxicillin, trimethoprim, and erythromycin. Combining toxicity, phenotypic and molecular endpoints, results showed differences in mortality, feeding, key enzyme activities and metabolic perturbations, thus supporting a distinct pattern in physiological responses of daphnids that could be used to monitor and predict pollution early.http://www.sciencedirect.com/science/article/pii/S2666911024000388PharmaceuticalsDaphnidsEnzyme markersFeedingPhenotypic endpointsMetabolomics |
| spellingShingle | Emma Rowan Anne Leung Katie O’Rourke Xiaofei Yin Lorraine Brennan Konstantinos Grintzalis New Approach Methodologies: Physiological responses of daphnids to pharmaceutical mixtures Journal of Hazardous Materials Letters Pharmaceuticals Daphnids Enzyme markers Feeding Phenotypic endpoints Metabolomics |
| title | New Approach Methodologies: Physiological responses of daphnids to pharmaceutical mixtures |
| title_full | New Approach Methodologies: Physiological responses of daphnids to pharmaceutical mixtures |
| title_fullStr | New Approach Methodologies: Physiological responses of daphnids to pharmaceutical mixtures |
| title_full_unstemmed | New Approach Methodologies: Physiological responses of daphnids to pharmaceutical mixtures |
| title_short | New Approach Methodologies: Physiological responses of daphnids to pharmaceutical mixtures |
| title_sort | new approach methodologies physiological responses of daphnids to pharmaceutical mixtures |
| topic | Pharmaceuticals Daphnids Enzyme markers Feeding Phenotypic endpoints Metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S2666911024000388 |
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