Potassium Current Signature of Neuronal/Glial Progenitors in Amniotic Fluid Stem Cells
Amniotic fluid is a complex and dynamic biological matrix that surrounds the fetus during the pregnancy. From this fluid, is possible to isolate various cell types with particular interest directed towards stem cells (AF-SCs). These cells are highly appealing due to their numerous potential applicat...
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2025-01-01
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| author | Paola Sabbatini Sabrina Cipriani Andrea Biagini Luana Sallicandro Cataldo Arcuri Rita Romani Paolo Prontera Alessandra Mirarchi Rosaria Gentile Diletta Del Bianco Elko Gliozheni Sandro Gerli Irene Giardina Maurizio Arduini Alessandro Favilli Antonio Malvasi Andrea Tinelli Bernard Fioretti |
| author_facet | Paola Sabbatini Sabrina Cipriani Andrea Biagini Luana Sallicandro Cataldo Arcuri Rita Romani Paolo Prontera Alessandra Mirarchi Rosaria Gentile Diletta Del Bianco Elko Gliozheni Sandro Gerli Irene Giardina Maurizio Arduini Alessandro Favilli Antonio Malvasi Andrea Tinelli Bernard Fioretti |
| author_sort | Paola Sabbatini |
| collection | DOAJ |
| description | Amniotic fluid is a complex and dynamic biological matrix that surrounds the fetus during the pregnancy. From this fluid, is possible to isolate various cell types with particular interest directed towards stem cells (AF-SCs). These cells are highly appealing due to their numerous potential applications in the field of regenerative medicine for tissues and organs as well as for treating conditions such as traumatic or ischemic injuries to the nervous system, myocardial infarction, or cancer. AF-SCs, when subcultured in the presence of basic Fibroblast Growth Factor (bFGF), have been shown to survive and migrate when transplanted into the striatum of the rat brain, exhibiting behavior characteristics of neuronal/glial progenitor cells. In this work, we performed an electrophysiological characterization to ascertain the propensity of AF-SCs to differentiate into glial and neuronal cells by bFGF. By using patch clamp technique we characterized a fibroblast-like morphology that display a barium-sensitive inward-rectifying potassium current (Kir) and calcium-activated potassium currents (KCa). The electrophysiological and calcium dynamics of histamine, a marker of undifferentiated neural progenitors, was further studied. Histamine promoted intracellular calcium increase by Fura-2 recording and calcium-activated potassium current activation with a similar temporal profile in AF-SC. The data presented in this paper ultimately confirm the expression in AF-SCs of the Kir and KCa currents, also showing regulation by endogenous stimuli such as histamine for the latter. |
| format | Article |
| id | doaj-art-211e6bbdf15849019ec233e684a1fa14 |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-211e6bbdf15849019ec233e684a1fa142025-01-10T13:16:22ZengMDPI AGCells2073-44092025-01-011415010.3390/cells14010050Potassium Current Signature of Neuronal/Glial Progenitors in Amniotic Fluid Stem CellsPaola Sabbatini0Sabrina Cipriani1Andrea Biagini2Luana Sallicandro3Cataldo Arcuri4Rita Romani5Paolo Prontera6Alessandra Mirarchi7Rosaria Gentile8Diletta Del Bianco9Elko Gliozheni10Sandro Gerli11Irene Giardina12Maurizio Arduini13Alessandro Favilli14Antonio Malvasi15Andrea Tinelli16Bernard Fioretti17Department of Chemistry, Biology and Biotechnologies, University of Perugia, Via dell’Elce di Sotto 8, 06123 Perugia, ItalyRheumatology Unit, Department of Medicine, School of Medicine, University of Perugia, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnologies, University of Perugia, Via dell’Elce di Sotto 8, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnologies, University of Perugia, Via dell’Elce di Sotto 8, 06123 Perugia, ItalyDepartment of Medicine and Surgery, University of Perugia, Piazza L. Severi 1, 06132 Perugia, ItalyDepartment of Medicine and Surgery, University of Perugia, Piazza L. Severi 1, 06132 Perugia, ItalyDepartment of Medicine and Surgery, University of Perugia, Piazza L. Severi 1, 06132 Perugia, ItalyDepartment of Medicine and Surgery, University of Perugia, Piazza L. Severi 1, 06132 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnologies, University of Perugia, Via dell’Elce di Sotto 8, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnologies, University of Perugia, Via dell’Elce di Sotto 8, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnologies, University of Perugia, Via dell’Elce di Sotto 8, 06123 Perugia, ItalyDepartment of Medicine and Surgery, University of Perugia, Piazza L. Severi 1, 06132 Perugia, ItalyDepartment of Medicine and Surgery, University of Perugia, Piazza L. Severi 1, 06132 Perugia, ItalyDepartment of Medicine and Surgery, University of Perugia, Piazza L. Severi 1, 06132 Perugia, ItalyDepartment of Medicine and Surgery, University of Perugia, Piazza L. Severi 1, 06132 Perugia, ItalyDepartment of Biomedical Sciences and Human Oncology, University of Bari, 70121 Bari, ItalyDepartment of Obstetrics and Gynecology and CERICSAL (CEntro di RIcerca Clinico SALentino), Veris delli Ponti Hospital, Via Giuseppina delli Ponti, 73020 Scorrano, ItalyDepartment of Chemistry, Biology and Biotechnologies, University of Perugia, Via dell’Elce di Sotto 8, 06123 Perugia, ItalyAmniotic fluid is a complex and dynamic biological matrix that surrounds the fetus during the pregnancy. From this fluid, is possible to isolate various cell types with particular interest directed towards stem cells (AF-SCs). These cells are highly appealing due to their numerous potential applications in the field of regenerative medicine for tissues and organs as well as for treating conditions such as traumatic or ischemic injuries to the nervous system, myocardial infarction, or cancer. AF-SCs, when subcultured in the presence of basic Fibroblast Growth Factor (bFGF), have been shown to survive and migrate when transplanted into the striatum of the rat brain, exhibiting behavior characteristics of neuronal/glial progenitor cells. In this work, we performed an electrophysiological characterization to ascertain the propensity of AF-SCs to differentiate into glial and neuronal cells by bFGF. By using patch clamp technique we characterized a fibroblast-like morphology that display a barium-sensitive inward-rectifying potassium current (Kir) and calcium-activated potassium currents (KCa). The electrophysiological and calcium dynamics of histamine, a marker of undifferentiated neural progenitors, was further studied. Histamine promoted intracellular calcium increase by Fura-2 recording and calcium-activated potassium current activation with a similar temporal profile in AF-SC. The data presented in this paper ultimately confirm the expression in AF-SCs of the Kir and KCa currents, also showing regulation by endogenous stimuli such as histamine for the latter.https://www.mdpi.com/2073-4409/14/1/50amniotic fluidneuronal/glial progenitorsneuronal differentiationpotassium channelscell-based therapy |
| spellingShingle | Paola Sabbatini Sabrina Cipriani Andrea Biagini Luana Sallicandro Cataldo Arcuri Rita Romani Paolo Prontera Alessandra Mirarchi Rosaria Gentile Diletta Del Bianco Elko Gliozheni Sandro Gerli Irene Giardina Maurizio Arduini Alessandro Favilli Antonio Malvasi Andrea Tinelli Bernard Fioretti Potassium Current Signature of Neuronal/Glial Progenitors in Amniotic Fluid Stem Cells Cells amniotic fluid neuronal/glial progenitors neuronal differentiation potassium channels cell-based therapy |
| title | Potassium Current Signature of Neuronal/Glial Progenitors in Amniotic Fluid Stem Cells |
| title_full | Potassium Current Signature of Neuronal/Glial Progenitors in Amniotic Fluid Stem Cells |
| title_fullStr | Potassium Current Signature of Neuronal/Glial Progenitors in Amniotic Fluid Stem Cells |
| title_full_unstemmed | Potassium Current Signature of Neuronal/Glial Progenitors in Amniotic Fluid Stem Cells |
| title_short | Potassium Current Signature of Neuronal/Glial Progenitors in Amniotic Fluid Stem Cells |
| title_sort | potassium current signature of neuronal glial progenitors in amniotic fluid stem cells |
| topic | amniotic fluid neuronal/glial progenitors neuronal differentiation potassium channels cell-based therapy |
| url | https://www.mdpi.com/2073-4409/14/1/50 |
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