Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J mice
In the present study we investigated the impact of the combination of astragalin and rutin (CAR) on restoring gut-microbial dysbiosis and obesity and obesity related disorders. Randomized male C57BL/6J mice were experimentally divided into 5 groups and fed either a normal diet or a high-fat diet (HF...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-11-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2023.9250012 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841550086103891968 |
|---|---|
| author | Muni Swamy Ganjayi Karunakaran Reddy Sankaran Balaji Meriga Ruchika Bhatia Shikha Sharma Kanthi Kiran Kondepudi |
| author_facet | Muni Swamy Ganjayi Karunakaran Reddy Sankaran Balaji Meriga Ruchika Bhatia Shikha Sharma Kanthi Kiran Kondepudi |
| author_sort | Muni Swamy Ganjayi |
| collection | DOAJ |
| description | In the present study we investigated the impact of the combination of astragalin and rutin (CAR) on restoring gut-microbial dysbiosis and obesity and obesity related disorders. Randomized male C57BL/6J mice were experimentally divided into 5 groups and fed either a normal diet or a high-fat diet (HFD) for 16 weeks. Compared to vehicle treated group (HFD group), CAR could substantially improve selected gut microbiota abundance (Akkermansia, Lactobacillus, Bifidobacteria, Roseburia, Prevotella), reversed the Firmicutes/Bacteroidetes proportions, and inhibited the growth of Escherichia coli, Salmonella, and Klebsiella in obese mice. In addition, CAR-treated mice showed significantly increased total short-chain fatty acid production, reduced body weight gain, organs’ weights, serum lipid profile (except HDL) and insulin resistance. The mRNA expressions of CCAAT/enhancer binding protein-α (C/EBP-α), sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-γ (PPAR-γ), acetyl-CoA carboxylase (ACC), adipocyte protein 2 (aP2), and fatty acid synthase (FAS) were downregulated (P < 0.05) and the protein expression of PPAR-γ was downregulated while adenosine 5’ monophosphate-activated protein kinase (AMPK) was phosphorylated in CAR-treated HFD-fed mice compared to the HFD control group. Interestingly, CAR-treated HFD-fed mice showed significantly improved tissue architecture in the liver and fatty tissues. In conclusion, the findings suggest that CAR/Moringa oleifera may be beneficial in the treatment of insulin resistance and obesity disorders. |
| format | Article |
| id | doaj-art-20e856301a8d4dcbbf2e19f1da6bb3d5 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-20e856301a8d4dcbbf2e19f1da6bb3d52025-01-10T06:57:02ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-11-011363256326510.26599/FSHW.2023.9250012Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J miceMuni Swamy Ganjayi0Karunakaran Reddy Sankaran1Balaji Meriga2Ruchika Bhatia3Shikha Sharma4Kanthi Kiran Kondepudi5Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, IndiaDepartment of Biochemistry, Sri Venkateswara University, Tirupati 517502, IndiaDepartment of Biochemistry, Sri Venkateswara University, Tirupati 517502, IndiaFood and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute, SAS Nagar, Punjab 140306, IndiaFood and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute, SAS Nagar, Punjab 140306, IndiaFood and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute, SAS Nagar, Punjab 140306, IndiaIn the present study we investigated the impact of the combination of astragalin and rutin (CAR) on restoring gut-microbial dysbiosis and obesity and obesity related disorders. Randomized male C57BL/6J mice were experimentally divided into 5 groups and fed either a normal diet or a high-fat diet (HFD) for 16 weeks. Compared to vehicle treated group (HFD group), CAR could substantially improve selected gut microbiota abundance (Akkermansia, Lactobacillus, Bifidobacteria, Roseburia, Prevotella), reversed the Firmicutes/Bacteroidetes proportions, and inhibited the growth of Escherichia coli, Salmonella, and Klebsiella in obese mice. In addition, CAR-treated mice showed significantly increased total short-chain fatty acid production, reduced body weight gain, organs’ weights, serum lipid profile (except HDL) and insulin resistance. The mRNA expressions of CCAAT/enhancer binding protein-α (C/EBP-α), sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-γ (PPAR-γ), acetyl-CoA carboxylase (ACC), adipocyte protein 2 (aP2), and fatty acid synthase (FAS) were downregulated (P < 0.05) and the protein expression of PPAR-γ was downregulated while adenosine 5’ monophosphate-activated protein kinase (AMPK) was phosphorylated in CAR-treated HFD-fed mice compared to the HFD control group. Interestingly, CAR-treated HFD-fed mice showed significantly improved tissue architecture in the liver and fatty tissues. In conclusion, the findings suggest that CAR/Moringa oleifera may be beneficial in the treatment of insulin resistance and obesity disorders.https://www.sciopen.com/article/10.26599/FSHW.2023.9250012astragalingut microbiotamoringa oleiferaobesityrutin |
| spellingShingle | Muni Swamy Ganjayi Karunakaran Reddy Sankaran Balaji Meriga Ruchika Bhatia Shikha Sharma Kanthi Kiran Kondepudi Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J mice Food Science and Human Wellness astragalin gut microbiota moringa oleifera obesity rutin |
| title | Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J mice |
| title_full | Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J mice |
| title_fullStr | Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J mice |
| title_full_unstemmed | Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J mice |
| title_short | Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J mice |
| title_sort | astragalin and rutin restore gut microbiota dysbiosis alleviate obesity and insulin resistance in high fat diet fed c57bl 6j mice |
| topic | astragalin gut microbiota moringa oleifera obesity rutin |
| url | https://www.sciopen.com/article/10.26599/FSHW.2023.9250012 |
| work_keys_str_mv | AT muniswamyganjayi astragalinandrutinrestoregutmicrobiotadysbiosisalleviateobesityandinsulinresistanceinhighfatdietfedc57bl6jmice AT karunakaranreddysankaran astragalinandrutinrestoregutmicrobiotadysbiosisalleviateobesityandinsulinresistanceinhighfatdietfedc57bl6jmice AT balajimeriga astragalinandrutinrestoregutmicrobiotadysbiosisalleviateobesityandinsulinresistanceinhighfatdietfedc57bl6jmice AT ruchikabhatia astragalinandrutinrestoregutmicrobiotadysbiosisalleviateobesityandinsulinresistanceinhighfatdietfedc57bl6jmice AT shikhasharma astragalinandrutinrestoregutmicrobiotadysbiosisalleviateobesityandinsulinresistanceinhighfatdietfedc57bl6jmice AT kanthikirankondepudi astragalinandrutinrestoregutmicrobiotadysbiosisalleviateobesityandinsulinresistanceinhighfatdietfedc57bl6jmice |