Speeding wheat breeding using super-long lighting and immature embryo culture in vitro

Wheat is relatively long from germination to seed maturity, and normally only one generation can be produced annually. Shortening the whole growth period, consequently producing seeds of multiple generations per year, is beneficial for improving wheat breeding efficiency. Low temperature (vernalizat...

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Main Authors: ZHENG Nannan, LU Xiangqian, ZHAO Junheng, FU Yujie, YE Lingzhen
Format: Article
Language:English
Published: Zhejiang University Press 2024-12-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.10.201
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author ZHENG Nannan
LU Xiangqian
ZHAO Junheng
FU Yujie
YE Lingzhen
author_facet ZHENG Nannan
LU Xiangqian
ZHAO Junheng
FU Yujie
YE Lingzhen
author_sort ZHENG Nannan
collection DOAJ
description Wheat is relatively long from germination to seed maturity, and normally only one generation can be produced annually. Shortening the whole growth period, consequently producing seeds of multiple generations per year, is beneficial for improving wheat breeding efficiency. Low temperature (vernalization response) and light length (photoperiod response) are the two key factors affecting the processes of wheat growth and development, or the whole growth period. On the basis of the relevant characteristics, we conducted different photoperiod and low-temperature vernalization treatments on wheat plants with different growth habits. Through observations of the growth stages, we found that super-long lighting (22 h light/2 h darkness) significantly shortened the wheat growth period and had a compensatory vernalization effect. No significant differences in panicle length, number of effective tillers per plant, or thousand grain weight were detected between the super-long lighting treatment and the control (16 h light/8 h darkness). Immature embryo culture in vitro at different time after pollination revealed that the 12-day-old young embryo had a high germination rate. Accordingly, the combination of super-long lighting and immature embryo culture in vitro may obtain 6-7 and 3-4 generations per year for spring- and winter-habit wheats, respectively. In conclusion, the method of speeding breeding developed in this study can be used in wheat breeding programs to improve breeding efficiency.
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publishDate 2024-12-01
publisher Zhejiang University Press
record_format Article
series 浙江大学学报. 农业与生命科学版
spelling doaj-art-20d0cedc1b3743dea0cfef2b801a9a052025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552024-12-015083884410.3785/j.issn.1008-9209.2023.10.20110089209Speeding wheat breeding using super-long lighting and immature embryo culture in vitroZHENG NannanLU XiangqianZHAO JunhengFU YujieYE LingzhenWheat is relatively long from germination to seed maturity, and normally only one generation can be produced annually. Shortening the whole growth period, consequently producing seeds of multiple generations per year, is beneficial for improving wheat breeding efficiency. Low temperature (vernalization response) and light length (photoperiod response) are the two key factors affecting the processes of wheat growth and development, or the whole growth period. On the basis of the relevant characteristics, we conducted different photoperiod and low-temperature vernalization treatments on wheat plants with different growth habits. Through observations of the growth stages, we found that super-long lighting (22 h light/2 h darkness) significantly shortened the wheat growth period and had a compensatory vernalization effect. No significant differences in panicle length, number of effective tillers per plant, or thousand grain weight were detected between the super-long lighting treatment and the control (16 h light/8 h darkness). Immature embryo culture in vitro at different time after pollination revealed that the 12-day-old young embryo had a high germination rate. Accordingly, the combination of super-long lighting and immature embryo culture in vitro may obtain 6-7 and 3-4 generations per year for spring- and winter-habit wheats, respectively. In conclusion, the method of speeding breeding developed in this study can be used in wheat breeding programs to improve breeding efficiency.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.10.201wheatphotoperiodimmature embryo culturespeed breeding
spellingShingle ZHENG Nannan
LU Xiangqian
ZHAO Junheng
FU Yujie
YE Lingzhen
Speeding wheat breeding using super-long lighting and immature embryo culture in vitro
浙江大学学报. 农业与生命科学版
wheat
photoperiod
immature embryo culture
speed breeding
title Speeding wheat breeding using super-long lighting and immature embryo culture in vitro
title_full Speeding wheat breeding using super-long lighting and immature embryo culture in vitro
title_fullStr Speeding wheat breeding using super-long lighting and immature embryo culture in vitro
title_full_unstemmed Speeding wheat breeding using super-long lighting and immature embryo culture in vitro
title_short Speeding wheat breeding using super-long lighting and immature embryo culture in vitro
title_sort speeding wheat breeding using super long lighting and immature embryo culture in vitro
topic wheat
photoperiod
immature embryo culture
speed breeding
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.10.201
work_keys_str_mv AT zhengnannan speedingwheatbreedingusingsuperlonglightingandimmatureembryocultureinvitro
AT luxiangqian speedingwheatbreedingusingsuperlonglightingandimmatureembryocultureinvitro
AT zhaojunheng speedingwheatbreedingusingsuperlonglightingandimmatureembryocultureinvitro
AT fuyujie speedingwheatbreedingusingsuperlonglightingandimmatureembryocultureinvitro
AT yelingzhen speedingwheatbreedingusingsuperlonglightingandimmatureembryocultureinvitro