Effect of isolated compounds from Combretum trifoliatum on toxicity and detoxification enzymes in Nilaparvata lugens
Abstract The brown planthopper (BPH) Nilaparvata lugens (Stål) is a major insect pest of Oryza sativa that causes crop yield loss in tropical regions, including Thailand. In this study, the crude ethanolic extract of the leaves and branches of Combretum trifoliatum , its active isolated components,...
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2025-01-01
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| author | Nutchaya Kumrungsee Saksit Nobsathian Worakawee Chumworathayee Poonnanan Phankaen Benjawan Dunkhunthod Opender Koul Chatwadee Saiyaitong Vasakorn Bullangpoti |
| author_facet | Nutchaya Kumrungsee Saksit Nobsathian Worakawee Chumworathayee Poonnanan Phankaen Benjawan Dunkhunthod Opender Koul Chatwadee Saiyaitong Vasakorn Bullangpoti |
| author_sort | Nutchaya Kumrungsee |
| collection | DOAJ |
| description | Abstract The brown planthopper (BPH) Nilaparvata lugens (Stål) is a major insect pest of Oryza sativa that causes crop yield loss in tropical regions, including Thailand. In this study, the crude ethanolic extract of the leaves and branches of Combretum trifoliatum , its active isolated components, apigenin and camphor, and Finopril were tested for their ability to control the first to fifth instars of N. lugens. The C. trifoliatum crude extract and both allelochemicals showed insecticide potential (24 h-LC50 ~ 8.83–95.96 mg/L against each instar for crude extract), and their toxicity depended on the time of exposure. Camphor showed the higher efficacy (LD50 ~ 4.43 mg/L) and not different compared to Finopril. All plant compounds tested reduced carboxylesterase (CE) and glutathione-s-transferase (GST) activities. Camphor caused the greatest decreases in CE and GST activities after exposure, whereas apigenin induced a slight change in acetylcholinesterase activity. The results of the present study suggest that C. trifoliatum extract can be used as an insecticide to manage N. lugens populations. |
| format | Article |
| id | doaj-art-2055a3bd35a54212aa89ec76625a0589 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-2055a3bd35a54212aa89ec76625a05892025-01-05T12:16:22ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-024-83351-1Effect of isolated compounds from Combretum trifoliatum on toxicity and detoxification enzymes in Nilaparvata lugensNutchaya Kumrungsee0Saksit Nobsathian1Worakawee Chumworathayee2Poonnanan Phankaen3Benjawan Dunkhunthod4Opender Koul5Chatwadee Saiyaitong6Vasakorn Bullangpoti7Biology Department, Faculty of Science and Technology, Rajamangala University of TechnologyNakhonsawan Campus, Mahidol UniversityBiology Department, Faculty of Science and Technology, Rajamangala University of TechnologyValaya Alongkorn Rajabhat University under the Royal Patronage Sa kaeo, Valaya Alongkorn Rajabhat University Under the Royal PatronageThai Traditional Medicine Program, Faculty of Nursing and Allied Health Sciences, Phetchaburi Rajabhat UniversityInsect Biopesticide Research CentreAnimal Toxicology and Physiology Speciality Research Unit, Zoology Department, Faculty of Science, Kasetsart UniversityAnimal Toxicology and Physiology Speciality Research Unit, Zoology Department, Faculty of Science, Kasetsart UniversityAbstract The brown planthopper (BPH) Nilaparvata lugens (Stål) is a major insect pest of Oryza sativa that causes crop yield loss in tropical regions, including Thailand. In this study, the crude ethanolic extract of the leaves and branches of Combretum trifoliatum , its active isolated components, apigenin and camphor, and Finopril were tested for their ability to control the first to fifth instars of N. lugens. The C. trifoliatum crude extract and both allelochemicals showed insecticide potential (24 h-LC50 ~ 8.83–95.96 mg/L against each instar for crude extract), and their toxicity depended on the time of exposure. Camphor showed the higher efficacy (LD50 ~ 4.43 mg/L) and not different compared to Finopril. All plant compounds tested reduced carboxylesterase (CE) and glutathione-s-transferase (GST) activities. Camphor caused the greatest decreases in CE and GST activities after exposure, whereas apigenin induced a slight change in acetylcholinesterase activity. The results of the present study suggest that C. trifoliatum extract can be used as an insecticide to manage N. lugens populations.https://doi.org/10.1038/s41598-024-83351-1InsectBotanicalAllelochemicalAcute toxicityDetoxification enzyme |
| spellingShingle | Nutchaya Kumrungsee Saksit Nobsathian Worakawee Chumworathayee Poonnanan Phankaen Benjawan Dunkhunthod Opender Koul Chatwadee Saiyaitong Vasakorn Bullangpoti Effect of isolated compounds from Combretum trifoliatum on toxicity and detoxification enzymes in Nilaparvata lugens Scientific Reports Insect Botanical Allelochemical Acute toxicity Detoxification enzyme |
| title | Effect of isolated compounds from Combretum trifoliatum on toxicity and detoxification enzymes in Nilaparvata lugens |
| title_full | Effect of isolated compounds from Combretum trifoliatum on toxicity and detoxification enzymes in Nilaparvata lugens |
| title_fullStr | Effect of isolated compounds from Combretum trifoliatum on toxicity and detoxification enzymes in Nilaparvata lugens |
| title_full_unstemmed | Effect of isolated compounds from Combretum trifoliatum on toxicity and detoxification enzymes in Nilaparvata lugens |
| title_short | Effect of isolated compounds from Combretum trifoliatum on toxicity and detoxification enzymes in Nilaparvata lugens |
| title_sort | effect of isolated compounds from combretum trifoliatum on toxicity and detoxification enzymes in nilaparvata lugens |
| topic | Insect Botanical Allelochemical Acute toxicity Detoxification enzyme |
| url | https://doi.org/10.1038/s41598-024-83351-1 |
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