The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in mice
Effective subunit vaccines for tuberculosis (TB) must target antigenic components at various stages of infection. In this study, we constructed fusion proteins using secreted antigens from Mycobacterium tuberculosis (M. tuberculosis), specifically ESAT6, CFP10, MPT64, and Rv2645 from the proliferati...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1450124/full |
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| author | Pu He Juan Wang Daquan Tan Lina Hu Yanlin Ma Youjun Mi Youjun Mi Fei Li Tingting Zhang Yunjie Du Wenhua Zhang Jixi Li Lei Jiao Bingdong Zhu Bingdong Zhu |
| author_facet | Pu He Juan Wang Daquan Tan Lina Hu Yanlin Ma Youjun Mi Youjun Mi Fei Li Tingting Zhang Yunjie Du Wenhua Zhang Jixi Li Lei Jiao Bingdong Zhu Bingdong Zhu |
| author_sort | Pu He |
| collection | DOAJ |
| description | Effective subunit vaccines for tuberculosis (TB) must target antigenic components at various stages of infection. In this study, we constructed fusion proteins using secreted antigens from Mycobacterium tuberculosis (M. tuberculosis), specifically ESAT6, CFP10, MPT64, and Rv2645 from the proliferation stage, along with latency-associated antigens Rv1738 and Rv1978. The resulting fusion proteins, designated LT33 (ESAT6-CFP10-Rv1738) and LT28 (MPT6461-170-Rv19788-60-Rv264521-80), were combined with an adjuvant containing dimethyldioctadecylammonium bromide (DDA), polyriboinosinic polyribocytidylic acid (PolyI:C), and cholesterol to construct subunit vaccines. We evaluated the subunit vaccine effect in C57BL/6 mice and revealed that LT33 and LT28 exhibited strong immunogenicity and induced protective efficacy against aerosol challenge with M. tuberculosis H37Rv. Notably, the combination of LT33 and LT28 led to a significant reduction of 0.77 log10 colony-forming units (CFU) of H37Rv in the lungs compared to the adjuvant control group, highlighting their potential as promising candidates for subunit vaccine against M. tuberculosis infection. |
| format | Article |
| id | doaj-art-20117aa0265246f6a4d84f34b39efb5a |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-20117aa0265246f6a4d84f34b39efb5a2024-11-22T06:16:49ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-11-011510.3389/fimmu.2024.14501241450124The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in micePu He0Juan Wang1Daquan Tan2Lina Hu3Yanlin Ma4Youjun Mi5Youjun Mi6Fei Li7Tingting Zhang8Yunjie Du9Wenhua Zhang10Jixi Li11Lei Jiao12Bingdong Zhu13Bingdong Zhu14State Key Laboratory for Animal Disease Control and Prevention and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaLanzhou Institute of Biological Products, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaInstitute of Pathogenic Physiology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaLanzhou Institute of Biological Products, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaSchool of Life Science, Lanzhou University, Lanzhou, ChinaState Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, ChinaLanzhou Institute of Biological Products, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaCollege of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaEffective subunit vaccines for tuberculosis (TB) must target antigenic components at various stages of infection. In this study, we constructed fusion proteins using secreted antigens from Mycobacterium tuberculosis (M. tuberculosis), specifically ESAT6, CFP10, MPT64, and Rv2645 from the proliferation stage, along with latency-associated antigens Rv1738 and Rv1978. The resulting fusion proteins, designated LT33 (ESAT6-CFP10-Rv1738) and LT28 (MPT6461-170-Rv19788-60-Rv264521-80), were combined with an adjuvant containing dimethyldioctadecylammonium bromide (DDA), polyriboinosinic polyribocytidylic acid (PolyI:C), and cholesterol to construct subunit vaccines. We evaluated the subunit vaccine effect in C57BL/6 mice and revealed that LT33 and LT28 exhibited strong immunogenicity and induced protective efficacy against aerosol challenge with M. tuberculosis H37Rv. Notably, the combination of LT33 and LT28 led to a significant reduction of 0.77 log10 colony-forming units (CFU) of H37Rv in the lungs compared to the adjuvant control group, highlighting their potential as promising candidates for subunit vaccine against M. tuberculosis infection.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1450124/fullMycobacterium tuberculosissubunit vaccineantigenssecreted antigenlatency-associated antigen |
| spellingShingle | Pu He Juan Wang Daquan Tan Lina Hu Yanlin Ma Youjun Mi Youjun Mi Fei Li Tingting Zhang Yunjie Du Wenhua Zhang Jixi Li Lei Jiao Bingdong Zhu Bingdong Zhu The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in mice Frontiers in Immunology Mycobacterium tuberculosis subunit vaccine antigens secreted antigen latency-associated antigen |
| title | The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in mice |
| title_full | The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in mice |
| title_fullStr | The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in mice |
| title_full_unstemmed | The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in mice |
| title_short | The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in mice |
| title_sort | combination of mycobacterium tuberculosis fusion proteins lt33 and lt28 induced strong protective immunity in mice |
| topic | Mycobacterium tuberculosis subunit vaccine antigens secreted antigen latency-associated antigen |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1450124/full |
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