Recent advances in CRISPR/Cas systems-associated surface enhanced Raman spectroscopy biosensor towards nucleic acid detection
Nucleic acids play a decisive role in vital life processes, such as growth, heredity, and evolution, and exploring different types of nucleic acid detection techniques has always been a research hotspot worldwide. Compared to traditional nucleic acid analysis methods, surface-enhanced Raman spectros...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Medicine in Novel Technology and Devices |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590093524000523 |
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| author | Dongjie Zhang Siyuan He Zixu Wang Peirao Yan Huandi Li Xinyi Xu Qi Zeng Nan Wang Xueli Chen |
| author_facet | Dongjie Zhang Siyuan He Zixu Wang Peirao Yan Huandi Li Xinyi Xu Qi Zeng Nan Wang Xueli Chen |
| author_sort | Dongjie Zhang |
| collection | DOAJ |
| description | Nucleic acids play a decisive role in vital life processes, such as growth, heredity, and evolution, and exploring different types of nucleic acid detection techniques has always been a research hotspot worldwide. Compared to traditional nucleic acid analysis methods, surface-enhanced Raman spectroscopy (SERS) detection has advantages, such as high sensitivity, good specificity, narrow spectral bands, minimal sample requirement, simple operation, and resistance to interference from autofluorescence and water in biological samples. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system has gained widespread attention in the fields of gene editing and biosensors. Combining the sequence-specific recognition capability of the CRISPR/Cas system with the high sensitivity fingerprinting properties of SERS technology offers a simple, rapid, and sensitive method towards nucleic acid detection. Here, common SERS Nanotags methods for nucleic acid detection are introduced, including “sandwich assay”, “signal switch assay” and HCR signal amplification. Then, the building mechanism of CRISPR-associated SERS detection is summarized. CRISPR/Cas12a activated by crRNA can recognize and lock the complementary target double-stranded DNA (dsDNA), followed by cis-cleavage of the dsDNA and trans-cleavage of the adjacent linker ssDNA. The loss of SERS signal based on aggregation is finally used for highly sensitive detection of the target DNA. Lastly, some typical applications through the integration of CRISPR/Cas and SERS technology are introduced, such as HIV-1 dsDNA, HBV DNA and AFB1 etc. |
| format | Article |
| id | doaj-art-20002e8d62d74d90b7d6b333b3821f8e |
| institution | Kabale University |
| issn | 2590-0935 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Medicine in Novel Technology and Devices |
| spelling | doaj-art-20002e8d62d74d90b7d6b333b3821f8e2024-12-09T04:27:58ZengElsevierMedicine in Novel Technology and Devices2590-09352024-12-0124100336Recent advances in CRISPR/Cas systems-associated surface enhanced Raman spectroscopy biosensor towards nucleic acid detectionDongjie Zhang0Siyuan He1Zixu Wang2Peirao Yan3Huandi Li4Xinyi Xu5Qi Zeng6Nan Wang7Xueli Chen8Biomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education & Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xidian University, Xi'an, Shaanxi, 710126, China; Innovation Center for Advanced Medical Imaging and Intelligent Medicine, Guangzhou Institute of Technology, Xidian University, Guangzhou, Guangdong, 51055, ChinaBiomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education & Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xidian University, Xi'an, Shaanxi, 710126, ChinaBiomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education & Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xidian University, Xi'an, Shaanxi, 710126, ChinaBiomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education & Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xidian University, Xi'an, Shaanxi, 710126, ChinaBiomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education & Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xidian University, Xi'an, Shaanxi, 710126, ChinaBiomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education & Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xidian University, Xi'an, Shaanxi, 710126, ChinaBiomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education & Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xidian University, Xi'an, Shaanxi, 710126, ChinaBiomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education & Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xidian University, Xi'an, Shaanxi, 710126, ChinaBiomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education & Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, Xidian University, Xi'an, Shaanxi, 710126, China; Innovation Center for Advanced Medical Imaging and Intelligent Medicine, Guangzhou Institute of Technology, Xidian University, Guangzhou, Guangdong, 51055, China; Corresponding author. Biomedical Photonics and Molecular Imaging Laboratory, School of Life Science and Technology, Xidian University and Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, Xi'an, Shaanxi, 710126, China.Nucleic acids play a decisive role in vital life processes, such as growth, heredity, and evolution, and exploring different types of nucleic acid detection techniques has always been a research hotspot worldwide. Compared to traditional nucleic acid analysis methods, surface-enhanced Raman spectroscopy (SERS) detection has advantages, such as high sensitivity, good specificity, narrow spectral bands, minimal sample requirement, simple operation, and resistance to interference from autofluorescence and water in biological samples. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system has gained widespread attention in the fields of gene editing and biosensors. Combining the sequence-specific recognition capability of the CRISPR/Cas system with the high sensitivity fingerprinting properties of SERS technology offers a simple, rapid, and sensitive method towards nucleic acid detection. Here, common SERS Nanotags methods for nucleic acid detection are introduced, including “sandwich assay”, “signal switch assay” and HCR signal amplification. Then, the building mechanism of CRISPR-associated SERS detection is summarized. CRISPR/Cas12a activated by crRNA can recognize and lock the complementary target double-stranded DNA (dsDNA), followed by cis-cleavage of the dsDNA and trans-cleavage of the adjacent linker ssDNA. The loss of SERS signal based on aggregation is finally used for highly sensitive detection of the target DNA. Lastly, some typical applications through the integration of CRISPR/Cas and SERS technology are introduced, such as HIV-1 dsDNA, HBV DNA and AFB1 etc.http://www.sciencedirect.com/science/article/pii/S2590093524000523 |
| spellingShingle | Dongjie Zhang Siyuan He Zixu Wang Peirao Yan Huandi Li Xinyi Xu Qi Zeng Nan Wang Xueli Chen Recent advances in CRISPR/Cas systems-associated surface enhanced Raman spectroscopy biosensor towards nucleic acid detection Medicine in Novel Technology and Devices |
| title | Recent advances in CRISPR/Cas systems-associated surface enhanced Raman spectroscopy biosensor towards nucleic acid detection |
| title_full | Recent advances in CRISPR/Cas systems-associated surface enhanced Raman spectroscopy biosensor towards nucleic acid detection |
| title_fullStr | Recent advances in CRISPR/Cas systems-associated surface enhanced Raman spectroscopy biosensor towards nucleic acid detection |
| title_full_unstemmed | Recent advances in CRISPR/Cas systems-associated surface enhanced Raman spectroscopy biosensor towards nucleic acid detection |
| title_short | Recent advances in CRISPR/Cas systems-associated surface enhanced Raman spectroscopy biosensor towards nucleic acid detection |
| title_sort | recent advances in crispr cas systems associated surface enhanced raman spectroscopy biosensor towards nucleic acid detection |
| url | http://www.sciencedirect.com/science/article/pii/S2590093524000523 |
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