Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of αβ-Expressing Tumors
The use of short-wave infrared (SWIR) light for fluorescence bioimaging offers the advantage of reduced photon scattering and improved tissue penetration compared to traditional shorter wavelength imaging approaches. While several nanomaterials have been shown capable of generating SWIR emissions, r...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-09-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012118799131 |
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| _version_ | 1846092103344455680 |
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| author | Dominik Jan Naczynski PhD Jason H. Stafford PhD Silvan Türkcan PhD Cesare Jenkins PhD Ai Leen Koh PhD Conroy Sun PhD Lei Xing PhD |
| author_facet | Dominik Jan Naczynski PhD Jason H. Stafford PhD Silvan Türkcan PhD Cesare Jenkins PhD Ai Leen Koh PhD Conroy Sun PhD Lei Xing PhD |
| author_sort | Dominik Jan Naczynski PhD |
| collection | DOAJ |
| description | The use of short-wave infrared (SWIR) light for fluorescence bioimaging offers the advantage of reduced photon scattering and improved tissue penetration compared to traditional shorter wavelength imaging approaches. While several nanomaterials have been shown capable of generating SWIR emissions, rare-earth-doped nanoparticles (REs) have emerged as an exceptionally bright and biocompatible class of SWIR emitters. Here, we demonstrate SWIR imaging of REs for several applications, including lymphatic mapping, real-time monitoring of probe biodistribution, and molecular targeting of the α v β 3 integrin in a tumor model. We further quantified the resolution and depth penetration limits of SWIR light emitted by REs in a customized imaging unit engineered for SWIR imaging of live small animals. Our results indicate that SWIR light has broad utility for preclinical biomedical imaging and demonstrates the potential for molecular imaging using targeted REs. |
| format | Article |
| id | doaj-art-1ff0d95c803440bbac60f6543ed81a40 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-1ff0d95c803440bbac60f6543ed81a402025-01-02T21:31:16ZengSAGE PublishingMolecular Imaging1536-01212018-09-011710.1177/1536012118799131Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of αβ-Expressing TumorsDominik Jan Naczynski PhD0Jason H. Stafford PhD1Silvan Türkcan PhD2Cesare Jenkins PhD3Ai Leen Koh PhD4Conroy Sun PhD5Lei Xing PhD6 Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, CA, USA Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, CA, USA Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, CA, USA Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, CA, USA Stanford Nanocharacterization Laboratory, Stanford University, Palo Alto, CA, USA Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, USA Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, CA, USAThe use of short-wave infrared (SWIR) light for fluorescence bioimaging offers the advantage of reduced photon scattering and improved tissue penetration compared to traditional shorter wavelength imaging approaches. While several nanomaterials have been shown capable of generating SWIR emissions, rare-earth-doped nanoparticles (REs) have emerged as an exceptionally bright and biocompatible class of SWIR emitters. Here, we demonstrate SWIR imaging of REs for several applications, including lymphatic mapping, real-time monitoring of probe biodistribution, and molecular targeting of the α v β 3 integrin in a tumor model. We further quantified the resolution and depth penetration limits of SWIR light emitted by REs in a customized imaging unit engineered for SWIR imaging of live small animals. Our results indicate that SWIR light has broad utility for preclinical biomedical imaging and demonstrates the potential for molecular imaging using targeted REs.https://doi.org/10.1177/1536012118799131 |
| spellingShingle | Dominik Jan Naczynski PhD Jason H. Stafford PhD Silvan Türkcan PhD Cesare Jenkins PhD Ai Leen Koh PhD Conroy Sun PhD Lei Xing PhD Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of αβ-Expressing Tumors Molecular Imaging |
| title | Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of αβ-Expressing Tumors |
| title_full | Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of αβ-Expressing Tumors |
| title_fullStr | Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of αβ-Expressing Tumors |
| title_full_unstemmed | Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of αβ-Expressing Tumors |
| title_short | Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of αβ-Expressing Tumors |
| title_sort | rare earth doped nanoparticles for short wave infrared fluorescence bioimaging and molecular targeting of αβ expressing tumors |
| url | https://doi.org/10.1177/1536012118799131 |
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