Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model
Background: The present study aims to evaluate the effect of local vitamin C application on inflammatory response and periodontal tissue destruction in rats with experimental periodontitis. Methods: A total of 21 animals, 7 rats in each group, were used in the study: 1- Non-ligation (NL), 2-experim...
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Selcuk University Press
2023-08-01
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| Series: | Selcuk Dental Journal |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/2422684 |
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| author | Kübra Karaçam Ayşe Toraman Zeliha Aytekin |
| author_facet | Kübra Karaçam Ayşe Toraman Zeliha Aytekin |
| author_sort | Kübra Karaçam |
| collection | DOAJ |
| description | Background: The present study aims to evaluate the effect of local vitamin C application on inflammatory response and periodontal tissue destruction in rats with experimental periodontitis.
Methods: A total of 21 animals, 7 rats in each group, were used in the study: 1- Non-ligation (NL), 2-experimental periodontitis (EP), and 3- local vitamin C (VtC) groups. Experimental periodontitis was induced in EP and VtC group rats. After 11 days, the ligature was removed. 50 mL of saline solution and vitamin C were injected locally into the vestibule sulcus of the EP and VtC group rats, respectively. After the rats were sacrificed, blood and gingival tissue samples were taken. TNF-α levels were analyzed biochemically in serum and 8-OHdG and MMP-8 were analyzed immunohistochemically in gingival tissue. The mandibular specimens were histologically evaluated for bone destruction and attachment loss.
Results: The numeric density of 8-OHdG and MMP-8 immunopositive cells were found to be statistically significantly lower in the Vtc group than in the EP group. Vitamin C administration significantly reduced attachment loss and bone loss in the VtC group as compared to the EP group. However, there was no significant difference in serum TNF-α level in the VtC group compared to the EP group.
Conclusions: Local application of vitamin C may reduce inflammation-related bone destruction by supporting the inflammatory response with the antioxidant activity of vitamin C and contributing to the resolution of inflammation with its potential immunomodulatory effect. Vitamin C may be a therapeutic agent that can be used in the treatment of periodontitis. |
| format | Article |
| id | doaj-art-1fecfe68eeee43448ac30dafeebaa661 |
| institution | Kabale University |
| issn | 2148-7529 |
| language | English |
| publishDate | 2023-08-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Selcuk Dental Journal |
| spelling | doaj-art-1fecfe68eeee43448ac30dafeebaa6612025-01-03T01:13:46ZengSelcuk University PressSelcuk Dental Journal2148-75292023-08-0110237137610.15311/selcukdentj.1115391154Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis ModelKübra Karaçam0Ayşe Toraman1Zeliha Aytekin2AFYONKARAHİSAR SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ, DİŞ HEKİMLİĞİ PR.SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE DİŞ HEKİMLİĞİ FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, PERİODONTOLOJİ ANABİLİM DALIAKDENIZ UNIVERSITY, FACULTY OF DENTISTRYBackground: The present study aims to evaluate the effect of local vitamin C application on inflammatory response and periodontal tissue destruction in rats with experimental periodontitis. Methods: A total of 21 animals, 7 rats in each group, were used in the study: 1- Non-ligation (NL), 2-experimental periodontitis (EP), and 3- local vitamin C (VtC) groups. Experimental periodontitis was induced in EP and VtC group rats. After 11 days, the ligature was removed. 50 mL of saline solution and vitamin C were injected locally into the vestibule sulcus of the EP and VtC group rats, respectively. After the rats were sacrificed, blood and gingival tissue samples were taken. TNF-α levels were analyzed biochemically in serum and 8-OHdG and MMP-8 were analyzed immunohistochemically in gingival tissue. The mandibular specimens were histologically evaluated for bone destruction and attachment loss. Results: The numeric density of 8-OHdG and MMP-8 immunopositive cells were found to be statistically significantly lower in the Vtc group than in the EP group. Vitamin C administration significantly reduced attachment loss and bone loss in the VtC group as compared to the EP group. However, there was no significant difference in serum TNF-α level in the VtC group compared to the EP group. Conclusions: Local application of vitamin C may reduce inflammation-related bone destruction by supporting the inflammatory response with the antioxidant activity of vitamin C and contributing to the resolution of inflammation with its potential immunomodulatory effect. Vitamin C may be a therapeutic agent that can be used in the treatment of periodontitis.https://dergipark.org.tr/tr/download/article-file/2422684antioxidantsexperimental periodontitisoxidative stressvitamin cantioksidanlardeneysel periodontitisoksidatif stresvitamin c |
| spellingShingle | Kübra Karaçam Ayşe Toraman Zeliha Aytekin Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model Selcuk Dental Journal antioxidants experimental periodontitis oxidative stress vitamin c antioksidanlar deneysel periodontitis oksidatif stres vitamin c |
| title | Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model |
| title_full | Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model |
| title_fullStr | Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model |
| title_full_unstemmed | Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model |
| title_short | Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model |
| title_sort | investigation of effects of local vitamin c application on inflammatory response and periodontal tissue destruction in rat periodontitis model |
| topic | antioxidants experimental periodontitis oxidative stress vitamin c antioksidanlar deneysel periodontitis oksidatif stres vitamin c |
| url | https://dergipark.org.tr/tr/download/article-file/2422684 |
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