Co-inoculation of arbuscular mycorrhizal fungi and Bacillus subtilis enhances morphological traits, growth, and nutrient uptake in maize under limited phosphorus availability
Abstract The use of beneficial microorganisms to enhance phosphate fertilizer use efficiency and solubilize residual phosphorus (P) is a promising strategy to improve soil P availability for plants. This study tested the hypothesis that inoculation with arbuscular mycorrhizal fungi, either alone or...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-10038-6 |
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| author | Helane Cristina de Andrade Rodrigues Severo Adriana Miranda de Santana Arauco Raimundo Wesley Fonseca Nunes Gilnair Nunes Monteiro Maria Helena Ferreira Duarte Allana Pereira Moura da Silva Adriele de Castro Ferreira Milena Rodrigues Luz Rafael de Souza Miranda Ademir Sérgio Ferreira de Araújo Elaine Martins da Costa |
| author_facet | Helane Cristina de Andrade Rodrigues Severo Adriana Miranda de Santana Arauco Raimundo Wesley Fonseca Nunes Gilnair Nunes Monteiro Maria Helena Ferreira Duarte Allana Pereira Moura da Silva Adriele de Castro Ferreira Milena Rodrigues Luz Rafael de Souza Miranda Ademir Sérgio Ferreira de Araújo Elaine Martins da Costa |
| author_sort | Helane Cristina de Andrade Rodrigues Severo |
| collection | DOAJ |
| description | Abstract The use of beneficial microorganisms to enhance phosphate fertilizer use efficiency and solubilize residual phosphorus (P) is a promising strategy to improve soil P availability for plants. This study tested the hypothesis that inoculation with arbuscular mycorrhizal fungi, either alone or in combination with Bacillus subtilis, enhances maize growth and optimizes nutrient availability, particularly phosphorus. The experiment was conducted under greenhouse conditions with four replications, following an 8 × 3 factorial design. Treatments included individual inoculations with Bacillus subtilis (IPACC26), Rhizophagus clarus (RJN102A), Claroideoglomus etunicatum (SCT101A), and a commercial inoculant of Rhizophagus intraradices (Rootella BR ULTRA), as well as three co-inoculations (IPACC26 combined with each fungus) and a non-inoculated control. These treatments were combined with three levels of phosphate fertilization (0, 50, and 100% of the recommended P level). Mycorrhizal colonization improved root architecture and increased photosynthetic pigments and uptake of P and other nutrients, resulting in greater plant growth and biomass production. The most pronounced effects were observed in plants inoculated with R. clarus and C. etunicatum, either alone or in combination with B. subtilis, at the 0 and 50% P levels. At 0% P, inoculated plants accumulated significantly more biomass, with root and shoot dry mass up to 3,000% and 680% higher, respectively, than those of uninoculated plants; this effect was associated with a 1,700% increase in shoot P accumulation compared to the control. These findings highlight the potential of these inoculants as biofertilizers for more sustainable and efficient phosphorus management in maize cultivation. |
| format | Article |
| id | doaj-art-1f8fb4c0349b4ba2b9737c172cc06cfe |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-1f8fb4c0349b4ba2b9737c172cc06cfe2025-08-20T04:01:51ZengNature PortfolioScientific Reports2045-23222025-07-0115111310.1038/s41598-025-10038-6Co-inoculation of arbuscular mycorrhizal fungi and Bacillus subtilis enhances morphological traits, growth, and nutrient uptake in maize under limited phosphorus availabilityHelane Cristina de Andrade Rodrigues Severo0Adriana Miranda de Santana Arauco1Raimundo Wesley Fonseca Nunes2Gilnair Nunes Monteiro3Maria Helena Ferreira Duarte4Allana Pereira Moura da Silva5Adriele de Castro Ferreira6Milena Rodrigues Luz7Rafael de Souza Miranda8Ademir Sérgio Ferreira de Araújo9Elaine Martins da Costa10Postgraduate Program in Agricultural Sciences, Federal University of PiauíCenter for Health Sciences, Federal University of PiauíAgronomic Engineering Course, Federal University of PiauíPostgraduate Program in Agricultural Sciences, Federal University of PiauíPostgraduate Program in Agricultural Sciences, Federal University of PiauíPostgraduate Program in Agricultural Sciences, Federal University of PiauíPostgraduate Program in Agricultural Sciences, Federal University of PiauíForest Engineering Course, Federal University of PiauíPostgraduate Program in Agricultural Sciences, Federal University of PiauíPostgraduate Program in Agricultural Sciences, Federal University of PiauíPostgraduate Program in Agricultural Sciences, Federal University of PiauíAbstract The use of beneficial microorganisms to enhance phosphate fertilizer use efficiency and solubilize residual phosphorus (P) is a promising strategy to improve soil P availability for plants. This study tested the hypothesis that inoculation with arbuscular mycorrhizal fungi, either alone or in combination with Bacillus subtilis, enhances maize growth and optimizes nutrient availability, particularly phosphorus. The experiment was conducted under greenhouse conditions with four replications, following an 8 × 3 factorial design. Treatments included individual inoculations with Bacillus subtilis (IPACC26), Rhizophagus clarus (RJN102A), Claroideoglomus etunicatum (SCT101A), and a commercial inoculant of Rhizophagus intraradices (Rootella BR ULTRA), as well as three co-inoculations (IPACC26 combined with each fungus) and a non-inoculated control. These treatments were combined with three levels of phosphate fertilization (0, 50, and 100% of the recommended P level). Mycorrhizal colonization improved root architecture and increased photosynthetic pigments and uptake of P and other nutrients, resulting in greater plant growth and biomass production. The most pronounced effects were observed in plants inoculated with R. clarus and C. etunicatum, either alone or in combination with B. subtilis, at the 0 and 50% P levels. At 0% P, inoculated plants accumulated significantly more biomass, with root and shoot dry mass up to 3,000% and 680% higher, respectively, than those of uninoculated plants; this effect was associated with a 1,700% increase in shoot P accumulation compared to the control. These findings highlight the potential of these inoculants as biofertilizers for more sustainable and efficient phosphorus management in maize cultivation.https://doi.org/10.1038/s41598-025-10038-6BiofertilizerPhosphate fertilizationNutrient solubilizationZea mays L |
| spellingShingle | Helane Cristina de Andrade Rodrigues Severo Adriana Miranda de Santana Arauco Raimundo Wesley Fonseca Nunes Gilnair Nunes Monteiro Maria Helena Ferreira Duarte Allana Pereira Moura da Silva Adriele de Castro Ferreira Milena Rodrigues Luz Rafael de Souza Miranda Ademir Sérgio Ferreira de Araújo Elaine Martins da Costa Co-inoculation of arbuscular mycorrhizal fungi and Bacillus subtilis enhances morphological traits, growth, and nutrient uptake in maize under limited phosphorus availability Scientific Reports Biofertilizer Phosphate fertilization Nutrient solubilization Zea mays L |
| title | Co-inoculation of arbuscular mycorrhizal fungi and Bacillus subtilis enhances morphological traits, growth, and nutrient uptake in maize under limited phosphorus availability |
| title_full | Co-inoculation of arbuscular mycorrhizal fungi and Bacillus subtilis enhances morphological traits, growth, and nutrient uptake in maize under limited phosphorus availability |
| title_fullStr | Co-inoculation of arbuscular mycorrhizal fungi and Bacillus subtilis enhances morphological traits, growth, and nutrient uptake in maize under limited phosphorus availability |
| title_full_unstemmed | Co-inoculation of arbuscular mycorrhizal fungi and Bacillus subtilis enhances morphological traits, growth, and nutrient uptake in maize under limited phosphorus availability |
| title_short | Co-inoculation of arbuscular mycorrhizal fungi and Bacillus subtilis enhances morphological traits, growth, and nutrient uptake in maize under limited phosphorus availability |
| title_sort | co inoculation of arbuscular mycorrhizal fungi and bacillus subtilis enhances morphological traits growth and nutrient uptake in maize under limited phosphorus availability |
| topic | Biofertilizer Phosphate fertilization Nutrient solubilization Zea mays L |
| url | https://doi.org/10.1038/s41598-025-10038-6 |
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