Fluoride stimulates the MAPK pathway to regulate endoplasmic reticulum stress and heat shock proteins to induce duodenal toxicity in chickens

ABSTRACT: Fluoride is one of the essential trace elements for body. However, excessive fluoride poses a major threat to human and animal health. Fluorosis may cause pathological damage of the duodenum, but the underlying mechanism needs to be further studied. This study was to investigate the effect...

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Main Authors: Yanan Li, Tianwen Ma, Xiaoping Lv, Zongsheng Qiu, Shuxin Li, Jingjing Qi, Chengwei Wei
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579124009878
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author Yanan Li
Tianwen Ma
Xiaoping Lv
Zongsheng Qiu
Shuxin Li
Jingjing Qi
Chengwei Wei
author_facet Yanan Li
Tianwen Ma
Xiaoping Lv
Zongsheng Qiu
Shuxin Li
Jingjing Qi
Chengwei Wei
author_sort Yanan Li
collection DOAJ
description ABSTRACT: Fluoride is one of the essential trace elements for body. However, excessive fluoride poses a major threat to human and animal health. Fluorosis may cause pathological damage of the duodenum, but the underlying mechanism needs to be further studied. This study was to investigate the effects of long-term exposure to sodium fluoride (0, 500, 1,000, 2,000 mg/kg) on the duodenum of chickens. The results showed that after NaF exposure, intestinal epithelial cells were disarranged, necrotic or even exfoliated, goblet cells and mucus secretion were increased, and inflammatory response was induced in duodenal tissue. Oxidative stress, endoplasmic reticulum stress (ERs), and heat shock proteins (HSPs) are an adaptive response, however long-term, excessive changes are detrimental. Fluorosis activates ERs through IRE1, PERK and ATF6 pathways, increases the expression of HSP60, HSP70 and HSP90, and causes apoptosis and oxidative damage in duodenal tissue. In addition, fluorosis can activate the MAPK signaling pathway. This article can provide a reference for exploring the potential duodenal toxicity of sodium fluoride.
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institution Kabale University
issn 0032-5791
language English
publishDate 2024-12-01
publisher Elsevier
record_format Article
series Poultry Science
spelling doaj-art-1f127d1e6b1b4880ba9c2e1a02f5bc3f2024-12-14T06:29:10ZengElsevierPoultry Science0032-57912024-12-0110312104408Fluoride stimulates the MAPK pathway to regulate endoplasmic reticulum stress and heat shock proteins to induce duodenal toxicity in chickensYanan Li0Tianwen Ma1Xiaoping Lv2Zongsheng Qiu3Shuxin Li4Jingjing Qi5Chengwei Wei6Heilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China; Animal Clinical Teaching Hospital, Northeast Agricultural University, Harbin, China; Corresponding author:ABSTRACT: Fluoride is one of the essential trace elements for body. However, excessive fluoride poses a major threat to human and animal health. Fluorosis may cause pathological damage of the duodenum, but the underlying mechanism needs to be further studied. This study was to investigate the effects of long-term exposure to sodium fluoride (0, 500, 1,000, 2,000 mg/kg) on the duodenum of chickens. The results showed that after NaF exposure, intestinal epithelial cells were disarranged, necrotic or even exfoliated, goblet cells and mucus secretion were increased, and inflammatory response was induced in duodenal tissue. Oxidative stress, endoplasmic reticulum stress (ERs), and heat shock proteins (HSPs) are an adaptive response, however long-term, excessive changes are detrimental. Fluorosis activates ERs through IRE1, PERK and ATF6 pathways, increases the expression of HSP60, HSP70 and HSP90, and causes apoptosis and oxidative damage in duodenal tissue. In addition, fluorosis can activate the MAPK signaling pathway. This article can provide a reference for exploring the potential duodenal toxicity of sodium fluoride.http://www.sciencedirect.com/science/article/pii/S0032579124009878fluorideMAPKendoplasmic reticulum stressheat shock proteinduodenal toxicity
spellingShingle Yanan Li
Tianwen Ma
Xiaoping Lv
Zongsheng Qiu
Shuxin Li
Jingjing Qi
Chengwei Wei
Fluoride stimulates the MAPK pathway to regulate endoplasmic reticulum stress and heat shock proteins to induce duodenal toxicity in chickens
Poultry Science
fluoride
MAPK
endoplasmic reticulum stress
heat shock protein
duodenal toxicity
title Fluoride stimulates the MAPK pathway to regulate endoplasmic reticulum stress and heat shock proteins to induce duodenal toxicity in chickens
title_full Fluoride stimulates the MAPK pathway to regulate endoplasmic reticulum stress and heat shock proteins to induce duodenal toxicity in chickens
title_fullStr Fluoride stimulates the MAPK pathway to regulate endoplasmic reticulum stress and heat shock proteins to induce duodenal toxicity in chickens
title_full_unstemmed Fluoride stimulates the MAPK pathway to regulate endoplasmic reticulum stress and heat shock proteins to induce duodenal toxicity in chickens
title_short Fluoride stimulates the MAPK pathway to regulate endoplasmic reticulum stress and heat shock proteins to induce duodenal toxicity in chickens
title_sort fluoride stimulates the mapk pathway to regulate endoplasmic reticulum stress and heat shock proteins to induce duodenal toxicity in chickens
topic fluoride
MAPK
endoplasmic reticulum stress
heat shock protein
duodenal toxicity
url http://www.sciencedirect.com/science/article/pii/S0032579124009878
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