Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice
Abstract Rice is susceptible to cold temperatures, especially during the seedling stage. Despite extensive research into the cold tolerance mechanisms of rice, the number of cloned genes remains limited. Plant subtilisin-like proteases (SUBs or SBTs) are protein-hydrolyzing enzymes which play import...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-84491-0 |
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| author | Jingyan Liu Fei He Zhicai Chen Meng Liu Yingni Xiao Ying Wang YuMeng Cai Jin Du Weiwei Jin Xuejun Liu |
| author_facet | Jingyan Liu Fei He Zhicai Chen Meng Liu Yingni Xiao Ying Wang YuMeng Cai Jin Du Weiwei Jin Xuejun Liu |
| author_sort | Jingyan Liu |
| collection | DOAJ |
| description | Abstract Rice is susceptible to cold temperatures, especially during the seedling stage. Despite extensive research into the cold tolerance mechanisms of rice, the number of cloned genes remains limited. Plant subtilisin-like proteases (SUBs or SBTs) are protein-hydrolyzing enzymes which play important roles in various aspects of plant growth as well as the plant response to biotic and abiotic stress. The rice SUB gene family consists of 62 members, but it is unknown whether they are involved in the response to cold stress. In this study, we observed that a loss-of-function SUB4 mutant exhibited enhanced cold tolerance at the seedling stage. The sub4 mutant seedlings exhibited improved survival rates and related physiological parameters, including relative electrolyte conductivity, chlorophyll content, malondialdehyde content, and antioxidant enzyme activity. Transcriptomic analysis revealed that differentially expressed genes responsive to cold stress in the sub4 mutants were primarily associated with metabolism and signal transduction. Notably, the majority of cold-responsive genes were associated with cell wall functions, including those related to cell wall organization, chitin catabolic processes, and oxidoreductases. Our findings suggest that SUB4 negatively regulates the cold response in rice seedlings, possibly by modifying the properties of the cell wall. |
| format | Article |
| id | doaj-art-1ed47072a0dd4cb3b01212bf386ab070 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-1ed47072a0dd4cb3b01212bf386ab0702025-01-05T12:16:37ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-024-84491-0Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in riceJingyan Liu0Fei He1Zhicai Chen2Meng Liu3Yingni Xiao4Ying Wang5YuMeng Cai6Jin Du7Weiwei Jin8Xuejun Liu9Tianjin Key Laboratory of Intelligent Breeding of Major Crops, College of Agronomy & Resources and Environment, Tianjin Agricultural UniversityTianjin Key Laboratory of Intelligent Breeding of Major Crops, College of Agronomy & Resources and Environment, Tianjin Agricultural UniversityTianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai UniversityTianjin Key Laboratory of Intelligent Breeding of Major Crops, College of Agronomy & Resources and Environment, Tianjin Agricultural UniversityGuangdong Provincial Key Laboratory of Crop Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural SciencesTianjin Key Laboratory of Intelligent Breeding of Major Crops, College of Agronomy & Resources and Environment, Tianjin Agricultural UniversityTianjin Key Laboratory of Intelligent Breeding of Major Crops, College of Agronomy & Resources and Environment, Tianjin Agricultural UniversityTianjin Key Laboratory of Intelligent Breeding of Major Crops, College of Agronomy & Resources and Environment, Tianjin Agricultural UniversityTianjin Key Laboratory of Intelligent Breeding of Major Crops, College of Agronomy & Resources and Environment, Tianjin Agricultural UniversityTianjin Key Laboratory of Intelligent Breeding of Major Crops, College of Agronomy & Resources and Environment, Tianjin Agricultural UniversityAbstract Rice is susceptible to cold temperatures, especially during the seedling stage. Despite extensive research into the cold tolerance mechanisms of rice, the number of cloned genes remains limited. Plant subtilisin-like proteases (SUBs or SBTs) are protein-hydrolyzing enzymes which play important roles in various aspects of plant growth as well as the plant response to biotic and abiotic stress. The rice SUB gene family consists of 62 members, but it is unknown whether they are involved in the response to cold stress. In this study, we observed that a loss-of-function SUB4 mutant exhibited enhanced cold tolerance at the seedling stage. The sub4 mutant seedlings exhibited improved survival rates and related physiological parameters, including relative electrolyte conductivity, chlorophyll content, malondialdehyde content, and antioxidant enzyme activity. Transcriptomic analysis revealed that differentially expressed genes responsive to cold stress in the sub4 mutants were primarily associated with metabolism and signal transduction. Notably, the majority of cold-responsive genes were associated with cell wall functions, including those related to cell wall organization, chitin catabolic processes, and oxidoreductases. Our findings suggest that SUB4 negatively regulates the cold response in rice seedlings, possibly by modifying the properties of the cell wall.https://doi.org/10.1038/s41598-024-84491-0 |
| spellingShingle | Jingyan Liu Fei He Zhicai Chen Meng Liu Yingni Xiao Ying Wang YuMeng Cai Jin Du Weiwei Jin Xuejun Liu Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice Scientific Reports |
| title | Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice |
| title_full | Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice |
| title_fullStr | Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice |
| title_full_unstemmed | Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice |
| title_short | Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice |
| title_sort | subtilisin like protease 4 regulates cold tolerance through cell wall modification in rice |
| url | https://doi.org/10.1038/s41598-024-84491-0 |
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