Biosynthesis of silver nanoparticles (AgNPs) from coconut leaf extract and their antifungal activity against Ganoderma boninense mycelia
Basal stem rot disease caused by Ganoderma is a major problem in palm cultivation in Indonesia, so an appropriate solution is needed to overcome this problem. One of the solutions that can be applied is through silver nanoparticles. Synthesis of silver nanoparticles can use coconut leaves as a sourc...
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indonesian research institute for biotechnology and bioindustry
2024-10-01
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| Series: | Menara Perkebunan |
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| Online Access: | https://mp.iribb.org/mpjurnal/article/view/586 |
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| author | Gusti Ayu Dewi Lestari I Gusti Ayu Made Megayanti Komang Ayu Astuti Maharani Ni Wayan Nieskajanti Kedeh Van Kempen |
| author_facet | Gusti Ayu Dewi Lestari I Gusti Ayu Made Megayanti Komang Ayu Astuti Maharani Ni Wayan Nieskajanti Kedeh Van Kempen |
| author_sort | Gusti Ayu Dewi Lestari |
| collection | DOAJ |
| description | Basal stem rot disease caused by Ganoderma is a major problem in palm cultivation in Indonesia, so an appropriate solution is needed to overcome this problem. One of the solutions that can be applied is through silver nanoparticles. Synthesis of silver nanoparticles can use coconut leaves as a source of flavonoids. Flavonoids are one of the phenolic compounds that are widely used in the process of synthesizing silver nanoparticles. Flavonoid compounds have a hydroxyl group (OH), which can be reduced by donating electrons to the Ag+ ion from AgNO3 to Ag0. This research aims to synthesize silver nanoparticles (AgNPs) using coconut leaf water extract and its assessment as an antifungal for Ganoderma boninense. Silver nanoparticles were synthesized by mixing coconut leaf extract in 1 mM of AgNO3 solution with a ratio of 1:9 (v/v). The concentrations of coconut leaf water extract were 1% and 3% with a heating temperature of 60°C. Silver nanoparticles were characterized using a UV-Vis spectrophotometer, PSA, FTIR, TEM, and SEM-EDS. The AgNPs had a maximum wavelength of 430 nm, with morphologies like a ball, triangle, and square, a mass percentage of Ag of 51.77%, smallest particle size of 63.29 nm, PdI value of 0.3361, and a zeta potential of -16.98 mV. The FTIR spectra show that the functional group that plays a role in the reduction process is the –OH group. The antifungal activity assay produced the highest percentage of colony growth inhibition (68.37%) at a concentration of 4.9 ppm in the 10-day incubation. |
| format | Article |
| id | doaj-art-1ec6b5aa1b9a4c2da518ad3b2c6c70eb |
| institution | Kabale University |
| issn | 0125-9318 1858-3768 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | indonesian research institute for biotechnology and bioindustry |
| record_format | Article |
| series | Menara Perkebunan |
| spelling | doaj-art-1ec6b5aa1b9a4c2da518ad3b2c6c70eb2024-11-25T07:16:35Zengindonesian research institute for biotechnology and bioindustryMenara Perkebunan0125-93181858-37682024-10-01922112122https://doi.org/10.22302/iribb.jur.mp.v92i2.586Biosynthesis of silver nanoparticles (AgNPs) from coconut leaf extract and their antifungal activity against Ganoderma boninense myceliaGusti Ayu Dewi Lestari0I Gusti Ayu Made Megayanti1Komang Ayu Astuti Maharani2Ni Wayan Nieskajanti Kedeh Van Kempen3 School of Pharmacy Mahaganesha, Denpasar, IndonesiaSchool of Pharmacy Mahaganesha, Denpasar, IndonesiaSchool of Pharmacy Mahaganesha, Denpasar, IndonesiaSchool of Pharmacy Mahaganesha, Denpasar, IndonesiaBasal stem rot disease caused by Ganoderma is a major problem in palm cultivation in Indonesia, so an appropriate solution is needed to overcome this problem. One of the solutions that can be applied is through silver nanoparticles. Synthesis of silver nanoparticles can use coconut leaves as a source of flavonoids. Flavonoids are one of the phenolic compounds that are widely used in the process of synthesizing silver nanoparticles. Flavonoid compounds have a hydroxyl group (OH), which can be reduced by donating electrons to the Ag+ ion from AgNO3 to Ag0. This research aims to synthesize silver nanoparticles (AgNPs) using coconut leaf water extract and its assessment as an antifungal for Ganoderma boninense. Silver nanoparticles were synthesized by mixing coconut leaf extract in 1 mM of AgNO3 solution with a ratio of 1:9 (v/v). The concentrations of coconut leaf water extract were 1% and 3% with a heating temperature of 60°C. Silver nanoparticles were characterized using a UV-Vis spectrophotometer, PSA, FTIR, TEM, and SEM-EDS. The AgNPs had a maximum wavelength of 430 nm, with morphologies like a ball, triangle, and square, a mass percentage of Ag of 51.77%, smallest particle size of 63.29 nm, PdI value of 0.3361, and a zeta potential of -16.98 mV. The FTIR spectra show that the functional group that plays a role in the reduction process is the –OH group. The antifungal activity assay produced the highest percentage of colony growth inhibition (68.37%) at a concentration of 4.9 ppm in the 10-day incubation.https://mp.iribb.org/mpjurnal/article/view/586antifungalbalicharacterizationcoconut leaf waste |
| spellingShingle | Gusti Ayu Dewi Lestari I Gusti Ayu Made Megayanti Komang Ayu Astuti Maharani Ni Wayan Nieskajanti Kedeh Van Kempen Biosynthesis of silver nanoparticles (AgNPs) from coconut leaf extract and their antifungal activity against Ganoderma boninense mycelia Menara Perkebunan antifungal bali characterization coconut leaf waste |
| title | Biosynthesis of silver nanoparticles (AgNPs) from coconut leaf extract and their antifungal activity against Ganoderma boninense mycelia |
| title_full | Biosynthesis of silver nanoparticles (AgNPs) from coconut leaf extract and their antifungal activity against Ganoderma boninense mycelia |
| title_fullStr | Biosynthesis of silver nanoparticles (AgNPs) from coconut leaf extract and their antifungal activity against Ganoderma boninense mycelia |
| title_full_unstemmed | Biosynthesis of silver nanoparticles (AgNPs) from coconut leaf extract and their antifungal activity against Ganoderma boninense mycelia |
| title_short | Biosynthesis of silver nanoparticles (AgNPs) from coconut leaf extract and their antifungal activity against Ganoderma boninense mycelia |
| title_sort | biosynthesis of silver nanoparticles agnps from coconut leaf extract and their antifungal activity against ganoderma boninense mycelia |
| topic | antifungal bali characterization coconut leaf waste |
| url | https://mp.iribb.org/mpjurnal/article/view/586 |
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