Antibacterial activity of endolysin LysP70 from Listeria monocytogenes phage
Endolysins, which are potential antimicrobial agents, can directly lyse gram-positive bacteria from the exterior. In this study, the endolysin gene derived from Listeria phage P70 was cloned, expressed, and purified, and designated LysP70. The antibacterial efficacy of LysP70 was comprehensively ass...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1566041/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849318844381790208 |
|---|---|
| author | Kunzhong Zhang Xuehui Zhao Qing Cao Qian Chong Ziqiu Fan Ji Zhi Jiabing He Jiayu Wang Zhonglong Wang Mingxia Cheng Min Xiao Zijian Wang Huiwen Xue Huitian Gou |
| author_facet | Kunzhong Zhang Xuehui Zhao Qing Cao Qian Chong Ziqiu Fan Ji Zhi Jiabing He Jiayu Wang Zhonglong Wang Mingxia Cheng Min Xiao Zijian Wang Huiwen Xue Huitian Gou |
| author_sort | Kunzhong Zhang |
| collection | DOAJ |
| description | Endolysins, which are potential antimicrobial agents, can directly lyse gram-positive bacteria from the exterior. In this study, the endolysin gene derived from Listeria phage P70 was cloned, expressed, and purified, and designated LysP70. The antibacterial efficacy of LysP70 was comprehensively assessed through plate counting and electron microscopy. The findings indicate that LysP70 is composed of 315 amino acids and has a molecular weight of 34.2 kDa, structural stability, and peptidase activity. Successfully expressed and purified LysP70 demonstrated lytic activity against L. monocytogenes, but not against Staphylococcus or Salmonella. LysP70 displayed stable enzymatic activity across a range of pH levels, temperatures, and metal ion concentrations. Furthermore, LysP70 significantly inhibited L. monocytogenes biofilm formation and scavenged existing biofilms, while affecting the transcriptional levels of genes associated with biofilm formation. In terms of food applications, LysP70 was effective in reducing the L. monocytogenes count in milk by 1.9 Log10 CFU/mL. This study offers a novel strategy for the prevention and control of L. monocytogenes infection, and establishes a theoretical basis for the development of endolysin antimicrobial agents. |
| format | Article |
| id | doaj-art-1dbde4b8546746bc9f6b4f6b48ed939c |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-1dbde4b8546746bc9f6b4f6b48ed939c2025-08-20T03:50:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.15660411566041Antibacterial activity of endolysin LysP70 from Listeria monocytogenes phageKunzhong Zhang0Xuehui Zhao1Qing Cao2Qian Chong3Ziqiu Fan4Ji Zhi5Jiabing He6Jiayu Wang7Zhonglong Wang8Mingxia Cheng9Min Xiao10Zijian Wang11Huiwen Xue12Huitian Gou13College of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaGansu Province Animal Disease Prevention and Control Center, Lanzhou, ChinaGansu Province Animal Disease Prevention and Control Center, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medical, Gansu Agricultural University, Lanzhou, ChinaEndolysins, which are potential antimicrobial agents, can directly lyse gram-positive bacteria from the exterior. In this study, the endolysin gene derived from Listeria phage P70 was cloned, expressed, and purified, and designated LysP70. The antibacterial efficacy of LysP70 was comprehensively assessed through plate counting and electron microscopy. The findings indicate that LysP70 is composed of 315 amino acids and has a molecular weight of 34.2 kDa, structural stability, and peptidase activity. Successfully expressed and purified LysP70 demonstrated lytic activity against L. monocytogenes, but not against Staphylococcus or Salmonella. LysP70 displayed stable enzymatic activity across a range of pH levels, temperatures, and metal ion concentrations. Furthermore, LysP70 significantly inhibited L. monocytogenes biofilm formation and scavenged existing biofilms, while affecting the transcriptional levels of genes associated with biofilm formation. In terms of food applications, LysP70 was effective in reducing the L. monocytogenes count in milk by 1.9 Log10 CFU/mL. This study offers a novel strategy for the prevention and control of L. monocytogenes infection, and establishes a theoretical basis for the development of endolysin antimicrobial agents.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1566041/fullLysP70antibacterial activityListeria monocytogenesendolysinbacteriophage |
| spellingShingle | Kunzhong Zhang Xuehui Zhao Qing Cao Qian Chong Ziqiu Fan Ji Zhi Jiabing He Jiayu Wang Zhonglong Wang Mingxia Cheng Min Xiao Zijian Wang Huiwen Xue Huitian Gou Antibacterial activity of endolysin LysP70 from Listeria monocytogenes phage Frontiers in Microbiology LysP70 antibacterial activity Listeria monocytogenes endolysin bacteriophage |
| title | Antibacterial activity of endolysin LysP70 from Listeria monocytogenes phage |
| title_full | Antibacterial activity of endolysin LysP70 from Listeria monocytogenes phage |
| title_fullStr | Antibacterial activity of endolysin LysP70 from Listeria monocytogenes phage |
| title_full_unstemmed | Antibacterial activity of endolysin LysP70 from Listeria monocytogenes phage |
| title_short | Antibacterial activity of endolysin LysP70 from Listeria monocytogenes phage |
| title_sort | antibacterial activity of endolysin lysp70 from listeria monocytogenes phage |
| topic | LysP70 antibacterial activity Listeria monocytogenes endolysin bacteriophage |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1566041/full |
| work_keys_str_mv | AT kunzhongzhang antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT xuehuizhao antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT qingcao antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT qianchong antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT ziqiufan antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT jizhi antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT jiabinghe antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT jiayuwang antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT zhonglongwang antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT mingxiacheng antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT minxiao antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT zijianwang antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT huiwenxue antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage AT huitiangou antibacterialactivityofendolysinlysp70fromlisteriamonocytogenesphage |