Construction of a Multicellular Communication Network Model for Cell Co-Culture Technology and Evaluation of Its Simulation Capability
Objective: Cell co-culture technology has been widely used to analyze the effects of drugs on cell proliferation and the expression of some proteins in cells, especially in the field of traditional Chinese medicine (TCM); however, the interactions between cells and the transmission of TCM effects be...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | World Journal of Traditional Chinese Medicine |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/wjtcm.wjtcm_73_24 |
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| _version_ | 1841526934598582272 |
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| author | Yuan-Yuan Geng Chao Wei Guo-Fei Chen Bai-Xia Zhang |
| author_facet | Yuan-Yuan Geng Chao Wei Guo-Fei Chen Bai-Xia Zhang |
| author_sort | Yuan-Yuan Geng |
| collection | DOAJ |
| description | Objective:
Cell co-culture technology has been widely used to analyze the effects of drugs on cell proliferation and the expression of some proteins in cells, especially in the field of traditional Chinese medicine (TCM); however, the interactions between cells and the transmission of TCM effects between cells have not been adequately studied.
Materials and Methods:
Using data on gene transcription regulation, biological response, signal channel, and cell-specific expression protein, we built a network for cell types based on entity grammar. Through the correspondence and location information of signal molecules and receptors, type-specific networks of single cells were connected and a multicellular network of smooth muscle cells, neurons, and vascular endothelial cells was constructed. The mechanism of action of nimodipine was analyzed based on the multicellular communication network and its simulation capability was evaluated.
Results:
The outputs generated by the model developed in this study showed that nimodipine inhibited smooth muscle contraction, due to the overload of Ca2+ and the toxicity of excitatory amino acids, and protected neurons and vascular endothelial cells by supporting cell proliferation and inhibiting cell apoptosis. These results were consistent with the known mechanism of nimodipine action, thus confirming that the multicellular network can be used to study the transmission of drug effects among cells.
Conclusions:
This study lays a foundation for the analysis of the transmission of drug effects in multi-cells, tissues, organs, and other spatial scales through multicellular co-culture experiments, based on a multicellular communication network. In addition, it provides a biological network model for the analysis of TCM action mechanisms. |
| format | Article |
| id | doaj-art-1daaceb973244cfdb4e8b4b38e54e9b0 |
| institution | Kabale University |
| issn | 2311-8571 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | World Journal of Traditional Chinese Medicine |
| spelling | doaj-art-1daaceb973244cfdb4e8b4b38e54e9b02025-01-16T08:46:29ZengWolters Kluwer Medknow PublicationsWorld Journal of Traditional Chinese Medicine2311-85712024-12-0110446547510.4103/wjtcm.wjtcm_73_24Construction of a Multicellular Communication Network Model for Cell Co-Culture Technology and Evaluation of Its Simulation CapabilityYuan-Yuan GengChao WeiGuo-Fei ChenBai-Xia ZhangObjective: Cell co-culture technology has been widely used to analyze the effects of drugs on cell proliferation and the expression of some proteins in cells, especially in the field of traditional Chinese medicine (TCM); however, the interactions between cells and the transmission of TCM effects between cells have not been adequately studied. Materials and Methods: Using data on gene transcription regulation, biological response, signal channel, and cell-specific expression protein, we built a network for cell types based on entity grammar. Through the correspondence and location information of signal molecules and receptors, type-specific networks of single cells were connected and a multicellular network of smooth muscle cells, neurons, and vascular endothelial cells was constructed. The mechanism of action of nimodipine was analyzed based on the multicellular communication network and its simulation capability was evaluated. Results: The outputs generated by the model developed in this study showed that nimodipine inhibited smooth muscle contraction, due to the overload of Ca2+ and the toxicity of excitatory amino acids, and protected neurons and vascular endothelial cells by supporting cell proliferation and inhibiting cell apoptosis. These results were consistent with the known mechanism of nimodipine action, thus confirming that the multicellular network can be used to study the transmission of drug effects among cells. Conclusions: This study lays a foundation for the analysis of the transmission of drug effects in multi-cells, tissues, organs, and other spatial scales through multicellular co-culture experiments, based on a multicellular communication network. In addition, it provides a biological network model for the analysis of TCM action mechanisms.https://journals.lww.com/10.4103/wjtcm.wjtcm_73_24cell co-culturecell type-specific networkdrug action mechanismentity grammar systemmulti-cell biological network model |
| spellingShingle | Yuan-Yuan Geng Chao Wei Guo-Fei Chen Bai-Xia Zhang Construction of a Multicellular Communication Network Model for Cell Co-Culture Technology and Evaluation of Its Simulation Capability World Journal of Traditional Chinese Medicine cell co-culture cell type-specific network drug action mechanism entity grammar system multi-cell biological network model |
| title | Construction of a Multicellular Communication Network Model for Cell Co-Culture Technology and Evaluation of Its Simulation Capability |
| title_full | Construction of a Multicellular Communication Network Model for Cell Co-Culture Technology and Evaluation of Its Simulation Capability |
| title_fullStr | Construction of a Multicellular Communication Network Model for Cell Co-Culture Technology and Evaluation of Its Simulation Capability |
| title_full_unstemmed | Construction of a Multicellular Communication Network Model for Cell Co-Culture Technology and Evaluation of Its Simulation Capability |
| title_short | Construction of a Multicellular Communication Network Model for Cell Co-Culture Technology and Evaluation of Its Simulation Capability |
| title_sort | construction of a multicellular communication network model for cell co culture technology and evaluation of its simulation capability |
| topic | cell co-culture cell type-specific network drug action mechanism entity grammar system multi-cell biological network model |
| url | https://journals.lww.com/10.4103/wjtcm.wjtcm_73_24 |
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