Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector
Companion animals, such as felines and canines, could provide an excellent platform for translational research from veterinary to human medicine. However, the use of feline induced pluripotent stems (fiPSCs) of quality in basic or clinical research has not been reported. Here, we generated footprint...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320424001494 |
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| _version_ | 1846148936378613760 |
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| author | Kazuto Kimura Masaya Tsukamoto Hiroko Sugisaki Miyuu Tanaka Mitsuru Kuwamura Yuki Matsumoto Genki Ishihara Kei Watanabe Mika Okada Mahito Nakanishi Kikuya Sugiura Shingo Hatoya |
| author_facet | Kazuto Kimura Masaya Tsukamoto Hiroko Sugisaki Miyuu Tanaka Mitsuru Kuwamura Yuki Matsumoto Genki Ishihara Kei Watanabe Mika Okada Mahito Nakanishi Kikuya Sugiura Shingo Hatoya |
| author_sort | Kazuto Kimura |
| collection | DOAJ |
| description | Companion animals, such as felines and canines, could provide an excellent platform for translational research from veterinary to human medicine. However, the use of feline induced pluripotent stems (fiPSCs) of quality in basic or clinical research has not been reported. Here, we generated footprint-free fiPSCs derived from embryonic cells, as well as juvenile feline uterus-derived cells using Sendai virus vector harboring six feline-specific pluripotency-associated genes. The fiPSCs were confirmed to be of high quality with the potential to form teratomas including all three germ layers. Furthermore, our fiPSCs were maintained under feeder-free and chemically-defined conditions using StemFit® AK02N and recombinant laminin 511, iMatrix-511. Further research on fiPSCs could result in their widespread application in veterinary regenerative medicine, which could pave the way for their use in advanced regenerative medicine research for humans. |
| format | Article |
| id | doaj-art-1d90ec70e8ce495bb603d67d954053e1 |
| institution | Kabale University |
| issn | 2352-3204 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-1d90ec70e8ce495bb603d67d954053e12024-11-30T07:10:56ZengElsevierRegenerative Therapy2352-32042024-06-0126708716Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vectorKazuto Kimura0Masaya Tsukamoto1Hiroko Sugisaki2Miyuu Tanaka3Mitsuru Kuwamura4Yuki Matsumoto5Genki Ishihara6Kei Watanabe7Mika Okada8Mahito Nakanishi9Kikuya Sugiura10Shingo Hatoya11Department of Advanced Pathobiology, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, JapanDepartment of Advanced Pathobiology, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, JapanDepartment of Advanced Pathobiology, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, JapanDepartment of Integrated Structural Biosciences, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, JapanDepartment of Integrated Structural Biosciences, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, JapanAnicom Specialty Medical Institute, Inc., Shinjuku-ku, Tokyo 231-0033, JapanAnicom Specialty Medical Institute, Inc., Shinjuku-ku, Tokyo 231-0033, JapanAnicom Specialty Medical Institute, Inc., Shinjuku-ku, Tokyo 231-0033, JapanTOKIWA-Bio, Inc., Tsukuba, Ibaraki 305-0047, JapanTOKIWA-Bio, Inc., Tsukuba, Ibaraki 305-0047, JapanDepartment of Advanced Pathobiology, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, JapanDepartment of Advanced Pathobiology, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, Japan; Corresponding author.Companion animals, such as felines and canines, could provide an excellent platform for translational research from veterinary to human medicine. However, the use of feline induced pluripotent stems (fiPSCs) of quality in basic or clinical research has not been reported. Here, we generated footprint-free fiPSCs derived from embryonic cells, as well as juvenile feline uterus-derived cells using Sendai virus vector harboring six feline-specific pluripotency-associated genes. The fiPSCs were confirmed to be of high quality with the potential to form teratomas including all three germ layers. Furthermore, our fiPSCs were maintained under feeder-free and chemically-defined conditions using StemFit® AK02N and recombinant laminin 511, iMatrix-511. Further research on fiPSCs could result in their widespread application in veterinary regenerative medicine, which could pave the way for their use in advanced regenerative medicine research for humans.http://www.sciencedirect.com/science/article/pii/S2352320424001494FelineInduced pluripotent stem cellsFootprint-freeSendai virus vectorFeeder-freeChemically-defined |
| spellingShingle | Kazuto Kimura Masaya Tsukamoto Hiroko Sugisaki Miyuu Tanaka Mitsuru Kuwamura Yuki Matsumoto Genki Ishihara Kei Watanabe Mika Okada Mahito Nakanishi Kikuya Sugiura Shingo Hatoya Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector Regenerative Therapy Feline Induced pluripotent stem cells Footprint-free Sendai virus vector Feeder-free Chemically-defined |
| title | Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector |
| title_full | Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector |
| title_fullStr | Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector |
| title_full_unstemmed | Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector |
| title_short | Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector |
| title_sort | generation of footprint free high quality feline induced pluripotent stem cells using sendai virus vector |
| topic | Feline Induced pluripotent stem cells Footprint-free Sendai virus vector Feeder-free Chemically-defined |
| url | http://www.sciencedirect.com/science/article/pii/S2352320424001494 |
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