Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China
Abstract Recently, a new bacterial disease was detected on cucumber stalks. In order to study the pathogenesis of this disease, the pathogenic bacteria were isolated and identified on the basis of morphological and molecular characteristics, and further analyzed for pathogenicity and antagonistic ev...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-84764-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559716078026752 |
|---|---|
| author | Yidan Wang Mengjun Jin Chengde Yang Fengfeng Cai Richard Osei Ting Ma Cuiwen Zhang Na Qi |
| author_facet | Yidan Wang Mengjun Jin Chengde Yang Fengfeng Cai Richard Osei Ting Ma Cuiwen Zhang Na Qi |
| author_sort | Yidan Wang |
| collection | DOAJ |
| description | Abstract Recently, a new bacterial disease was detected on cucumber stalks. In order to study the pathogenesis of this disease, the pathogenic bacteria were isolated and identified on the basis of morphological and molecular characteristics, and further analyzed for pathogenicity and antagonistic evaluation. Pathogenicity analysis showed that HlJ-3 caused melting decay and cracking in cucumber stems, and the strain reisolated from re-infected cucumber stalks was morphologically identical to HlJ-3 colonies, which is consistent with the Koch’s postulates. The pathogenic strain HlJ-3 was identified as having similar morphological characteristics to Bacillus subtilis. Meanwhile, its internal transcribed spacer sequence (ITS) and DNA gyrase A subunit (gyrA) were both more than 99% homologous and clustered on the same branch with B. subtilis. Therefore, combined with morphological and molecular biological features, strain HlJ-3 was identified as B. subtilis. In addition, B. subtilis, which has a wide range of hosts, was able to infest other common crop species, including potato, tomato, pepper, melon, and radish. Furthermore, antagonistic evaluation confirmed that strain HlJ-3 strongly inhibited the mycelial growth of Colletotrichum coccodes and Alternaria tenuissima in vitro, with antagonistic effects of 69.92% and 68.08%, respectively. In conclusion, our results showed that strain HlJ-3 is B. subtilis, which is pathogenic to cucumber in vivo and can infect plants of Solanaceae, Cucurbitaceae and Brassicaceae with a wide range of hosts. In addition, this strain has good biocontrol effects against C. coccodes and A. tenuissima in vitro. The findings of this research will help to prevent and control the occurrence of this pathogen and regulate its use as a biocontrol agent. |
| format | Article |
| id | doaj-art-1d334fc88da545d78da9b6d03d7442e2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-1d334fc88da545d78da9b6d03d7442e22025-01-05T12:15:08ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-024-84764-8Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in ChinaYidan Wang0Mengjun Jin1Chengde Yang2Fengfeng Cai3Richard Osei4Ting Ma5Cuiwen Zhang6Na Qi7College of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural UniversityCollege of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural UniversityCollege of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural UniversityCollege of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural UniversityCollege of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural UniversityCollege of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural UniversityCollege of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural UniversityCollege of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural UniversityAbstract Recently, a new bacterial disease was detected on cucumber stalks. In order to study the pathogenesis of this disease, the pathogenic bacteria were isolated and identified on the basis of morphological and molecular characteristics, and further analyzed for pathogenicity and antagonistic evaluation. Pathogenicity analysis showed that HlJ-3 caused melting decay and cracking in cucumber stems, and the strain reisolated from re-infected cucumber stalks was morphologically identical to HlJ-3 colonies, which is consistent with the Koch’s postulates. The pathogenic strain HlJ-3 was identified as having similar morphological characteristics to Bacillus subtilis. Meanwhile, its internal transcribed spacer sequence (ITS) and DNA gyrase A subunit (gyrA) were both more than 99% homologous and clustered on the same branch with B. subtilis. Therefore, combined with morphological and molecular biological features, strain HlJ-3 was identified as B. subtilis. In addition, B. subtilis, which has a wide range of hosts, was able to infest other common crop species, including potato, tomato, pepper, melon, and radish. Furthermore, antagonistic evaluation confirmed that strain HlJ-3 strongly inhibited the mycelial growth of Colletotrichum coccodes and Alternaria tenuissima in vitro, with antagonistic effects of 69.92% and 68.08%, respectively. In conclusion, our results showed that strain HlJ-3 is B. subtilis, which is pathogenic to cucumber in vivo and can infect plants of Solanaceae, Cucurbitaceae and Brassicaceae with a wide range of hosts. In addition, this strain has good biocontrol effects against C. coccodes and A. tenuissima in vitro. The findings of this research will help to prevent and control the occurrence of this pathogen and regulate its use as a biocontrol agent.https://doi.org/10.1038/s41598-024-84764-8Cucumis sativusGummy stem blight diseaseBacillus subtilisIsolationIdentificationPathogenicity |
| spellingShingle | Yidan Wang Mengjun Jin Chengde Yang Fengfeng Cai Richard Osei Ting Ma Cuiwen Zhang Na Qi Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China Scientific Reports Cucumis sativus Gummy stem blight disease Bacillus subtilis Isolation Identification Pathogenicity |
| title | Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China |
| title_full | Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China |
| title_fullStr | Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China |
| title_full_unstemmed | Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China |
| title_short | Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China |
| title_sort | isolation and identification of the causal agent of gummy stem blight disease in cucumis sativus caused by a bacterial pathogen in china |
| topic | Cucumis sativus Gummy stem blight disease Bacillus subtilis Isolation Identification Pathogenicity |
| url | https://doi.org/10.1038/s41598-024-84764-8 |
| work_keys_str_mv | AT yidanwang isolationandidentificationofthecausalagentofgummystemblightdiseaseincucumissativuscausedbyabacterialpathogeninchina AT mengjunjin isolationandidentificationofthecausalagentofgummystemblightdiseaseincucumissativuscausedbyabacterialpathogeninchina AT chengdeyang isolationandidentificationofthecausalagentofgummystemblightdiseaseincucumissativuscausedbyabacterialpathogeninchina AT fengfengcai isolationandidentificationofthecausalagentofgummystemblightdiseaseincucumissativuscausedbyabacterialpathogeninchina AT richardosei isolationandidentificationofthecausalagentofgummystemblightdiseaseincucumissativuscausedbyabacterialpathogeninchina AT tingma isolationandidentificationofthecausalagentofgummystemblightdiseaseincucumissativuscausedbyabacterialpathogeninchina AT cuiwenzhang isolationandidentificationofthecausalagentofgummystemblightdiseaseincucumissativuscausedbyabacterialpathogeninchina AT naqi isolationandidentificationofthecausalagentofgummystemblightdiseaseincucumissativuscausedbyabacterialpathogeninchina |