Serological and Molecular Diagnosis of Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry Farms
ABSTRACT The objective of this research was to evaluate the efficacy of serological and molecular tests in the detection of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in laying and broiler flocks. For this analysis, 344 blood serum samples, 220 tracheal swab samples, and 66 trachea s...
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Fundação APINCO de Ciência e Tecnologia Avícolas
2024-11-01
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| Series: | Brazilian Journal of Poultry Science |
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| author | MMR Amorim RP Bandeira SM Clemente S Vilela RA Mota ER Nascimento MR Barros |
| author_facet | MMR Amorim RP Bandeira SM Clemente S Vilela RA Mota ER Nascimento MR Barros |
| author_sort | MMR Amorim |
| collection | DOAJ |
| description | ABSTRACT The objective of this research was to evaluate the efficacy of serological and molecular tests in the detection of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in laying and broiler flocks. For this analysis, 344 blood serum samples, 220 tracheal swab samples, and 66 trachea samples were collected. The serum samples were subjected to a rapid agglutination (RSA) test, a hemagglutination inhibition (HI) assay, and an enzyme-linked immunosorbent assay (ELISA). Polymerase Chain Reaction (PCR), nPCR, and vaccine strain PCR were performed on the trachea and tracheal swab samples. RSA was conducted at 1:10 dilution, and resulted in 14.8% (51/344) of samples positive for MG and 28.5% (98/344) for MS. Regarding the results of the HI test, 8.7% (30/344) and 20.3% (70/344) were positive for MG and MS, respectively. Based on the ELISA, 45.6% (177/344) of the samples showed seropositivity for MG, and 57.3% (189/344) for MS. Out of all tracheal swab and trachea samples subjected to PCR, 25.9% (57/220) and 42.8% (31/66) were positive for MG, while 10.0% (22/220) and 18.2% (12/66) were positive for MS, respectively. As determined by the vaccine strain PCR, 56.1% (32/57) of the tracheal swab samples that were positive for MG originated from the MG-F strain, whereas, of the positive trachea samples, 40.6% (13/32) were positive for that vaccine strain. The importance of using different serological and molecular tools in the diagnosis of MG and MS is clear, considering the great variation of results between the techniques, in addition to the possibility that DNA of the agent detected in flocks not having presented seroconversion. |
| format | Article |
| id | doaj-art-1d060b22ae02443e8a18a845d1cc59e3 |
| institution | Kabale University |
| issn | 1806-9061 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| record_format | Article |
| series | Brazilian Journal of Poultry Science |
| spelling | doaj-art-1d060b22ae02443e8a18a845d1cc59e32024-11-26T07:46:15ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612024-11-0126310.1590/1806-9061-2024-1953Serological and Molecular Diagnosis of Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry FarmsMMR Amorimhttps://orcid.org/0000-0002-2305-4330RP Bandeirahttps://orcid.org/0000-0001-7162-2745SM Clementehttps://orcid.org/0000-0002-2528-9416S Vilelahttps://orcid.org/0009-0000-1780-9357RA Motahttps://orcid.org/0000-0002-2844-5509ER Nascimentohttps://orcid.org/0000-0003-2316-8933MR Barroshttps://orcid.org/0000-0003-3449-9164ABSTRACT The objective of this research was to evaluate the efficacy of serological and molecular tests in the detection of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in laying and broiler flocks. For this analysis, 344 blood serum samples, 220 tracheal swab samples, and 66 trachea samples were collected. The serum samples were subjected to a rapid agglutination (RSA) test, a hemagglutination inhibition (HI) assay, and an enzyme-linked immunosorbent assay (ELISA). Polymerase Chain Reaction (PCR), nPCR, and vaccine strain PCR were performed on the trachea and tracheal swab samples. RSA was conducted at 1:10 dilution, and resulted in 14.8% (51/344) of samples positive for MG and 28.5% (98/344) for MS. Regarding the results of the HI test, 8.7% (30/344) and 20.3% (70/344) were positive for MG and MS, respectively. Based on the ELISA, 45.6% (177/344) of the samples showed seropositivity for MG, and 57.3% (189/344) for MS. Out of all tracheal swab and trachea samples subjected to PCR, 25.9% (57/220) and 42.8% (31/66) were positive for MG, while 10.0% (22/220) and 18.2% (12/66) were positive for MS, respectively. As determined by the vaccine strain PCR, 56.1% (32/57) of the tracheal swab samples that were positive for MG originated from the MG-F strain, whereas, of the positive trachea samples, 40.6% (13/32) were positive for that vaccine strain. The importance of using different serological and molecular tools in the diagnosis of MG and MS is clear, considering the great variation of results between the techniques, in addition to the possibility that DNA of the agent detected in flocks not having presented seroconversion.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2024000300316&lng=en&tlng=enBroilercommercial egg layersSerologyPCRMycoplasma sp |
| spellingShingle | MMR Amorim RP Bandeira SM Clemente S Vilela RA Mota ER Nascimento MR Barros Serological and Molecular Diagnosis of Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry Farms Brazilian Journal of Poultry Science Broiler commercial egg layers Serology PCR Mycoplasma sp |
| title | Serological and Molecular Diagnosis of Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry Farms |
| title_full | Serological and Molecular Diagnosis of Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry Farms |
| title_fullStr | Serological and Molecular Diagnosis of Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry Farms |
| title_full_unstemmed | Serological and Molecular Diagnosis of Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry Farms |
| title_short | Serological and Molecular Diagnosis of Mycoplasma gallisepticum and Mycoplasma synoviae in Poultry Farms |
| title_sort | serological and molecular diagnosis of mycoplasma gallisepticum and mycoplasma synoviae in poultry farms |
| topic | Broiler commercial egg layers Serology PCR Mycoplasma sp |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2024000300316&lng=en&tlng=en |
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