A luminescent-based protocol for NAD+/NADH detection in C. elegans, mice, and human whole blood

A luminescent-based protocol for NAD+/NADH detection in C. elegans, mice, and human whole blood

Summary: Here, we present a NAD+/NADH detection assay for evaluating NAD+, NADH, and NAD+/NADH ratio across diverse biological models, including Caenorhabditis elegans, mouse muscle tissue, mouse whole blood, and human whole blood. We describe steps for sample collection and preparation from differe...

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Bibliographic Details
Main Authors: He-Ling Wang, Jianying Zhang, Shu-qin Cao, Maria Jose Lagartos-Donate, Shi-qi Zhang, Sofie Lautrup, Zeping Hu, Costas A. Lyssiotis, Riekelt H. Houtkooper, Evandro F. Fang
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Metabolism
Molecular Biology
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005938
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http://www.sciencedirect.com/science/article/pii/S2666166724005938

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