Effect of a chemically-modified-curcumin on dental resin biodegradation
IntroductionPrevious studies have shown Streptococcus mutans (S. mutans) esterase is a key mediator of dental composite biodegradation, which can contribute to recurrent caries. This study is to investigate the inhibitory effects of a novel Chemically-Modified-Curcumin (CMC 2.24) on esterase activit...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Oral Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/froh.2024.1506616/full |
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| author | Qi Dai Hsi-Ming Lee Austin Giordano Fu-Pen Chiang Stephen G. Walker Rafael Delgado-Ruiz Francis Johnson Lorne M. Golub Ying Gu |
| author_facet | Qi Dai Hsi-Ming Lee Austin Giordano Fu-Pen Chiang Stephen G. Walker Rafael Delgado-Ruiz Francis Johnson Lorne M. Golub Ying Gu |
| author_sort | Qi Dai |
| collection | DOAJ |
| description | IntroductionPrevious studies have shown Streptococcus mutans (S. mutans) esterase is a key mediator of dental composite biodegradation, which can contribute to recurrent caries. This study is to investigate the inhibitory effects of a novel Chemically-Modified-Curcumin (CMC 2.24) on esterase activities and related dental material biodegradation.MethodsDental adhesive materials and composite resins were incubated in S. mutans suspension with CMC 2.24 and other compounds, including doxycycline, Chemically-Modified-Tetracycline (CMT-3), and curcumin for 4 weeks. The pre- and post-incubation surface roughness were evaluated by either laser diffraction pattern and/or a 3D laser scanning microscope. Esterase enzyme inhibition assays were performed with the same test groups and activities were determined spectrophotometrically.ResultsAmong all experimental groups, CMC 2.24 significantly reduced surface roughness of dental composite (p < 0.01) and adhesive (p < 0.01) materials compared to bacteria-only group. Additionally, CMC 2.24 reduced porcine esterase activity by 46.5%, while other compounds showed minimal inhibition. In the S. mutans esterase assay, CMC 2.24 showed inhibition of 70.0%, while other compounds showed inhibition ranging from 19% to 36%.ConclusionOur study demonstrated that CMC 2.24 inhibited biodegradation of dental composite material more effectively than its mother compound, curcumin. Moreover, the mechanism of this biodegradation was likely mediated through bacterial esterase activity. Doxycycline achieved similar inhibition by completely eradicating S. mutans with its antibiotic action; hence, it is not recommended for long-term use. |
| format | Article |
| id | doaj-art-1ca5cd84bef94cab835f6e770cbfdeb8 |
| institution | Kabale University |
| issn | 2673-4842 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oral Health |
| spelling | doaj-art-1ca5cd84bef94cab835f6e770cbfdeb82025-01-14T06:10:46ZengFrontiers Media S.A.Frontiers in Oral Health2673-48422025-01-01510.3389/froh.2024.15066161506616Effect of a chemically-modified-curcumin on dental resin biodegradationQi Dai0Hsi-Ming Lee1Austin Giordano2Fu-Pen Chiang3Stephen G. Walker4Rafael Delgado-Ruiz5Francis Johnson6Lorne M. Golub7Ying Gu8Department of General Dentistry, School of Dental Medicine, Stony Brook University, Stony Brook, NY, United StatesDepartment of Oral Biology and Pathology, School of Dental Medicine, Stony Brook University, Stony Brook, NY, United StatesDepartment of Mechanical Engineering, College of Engineering and Applied Sciences, Stony Brook University, Stony Brook, NY, United StatesDepartment of Mechanical Engineering, College of Engineering and Applied Sciences, Stony Brook University, Stony Brook, NY, United StatesDepartment of Oral Biology and Pathology, School of Dental Medicine, Stony Brook University, Stony Brook, NY, United StatesDepartment of Prosthodontics and Digital Technology, School of Dental Medicine, Stony Brook University, Stony Brook, NY, United StatesDepartment of Chemistry and Pharmacological Sciences, School of Medicine, Stony Brook University, Stony Brook, NY, United StatesDepartment of Oral Biology and Pathology, School of Dental Medicine, Stony Brook University, Stony Brook, NY, United StatesDepartment of General Dentistry, School of Dental Medicine, Stony Brook University, Stony Brook, NY, United StatesIntroductionPrevious studies have shown Streptococcus mutans (S. mutans) esterase is a key mediator of dental composite biodegradation, which can contribute to recurrent caries. This study is to investigate the inhibitory effects of a novel Chemically-Modified-Curcumin (CMC 2.24) on esterase activities and related dental material biodegradation.MethodsDental adhesive materials and composite resins were incubated in S. mutans suspension with CMC 2.24 and other compounds, including doxycycline, Chemically-Modified-Tetracycline (CMT-3), and curcumin for 4 weeks. The pre- and post-incubation surface roughness were evaluated by either laser diffraction pattern and/or a 3D laser scanning microscope. Esterase enzyme inhibition assays were performed with the same test groups and activities were determined spectrophotometrically.ResultsAmong all experimental groups, CMC 2.24 significantly reduced surface roughness of dental composite (p < 0.01) and adhesive (p < 0.01) materials compared to bacteria-only group. Additionally, CMC 2.24 reduced porcine esterase activity by 46.5%, while other compounds showed minimal inhibition. In the S. mutans esterase assay, CMC 2.24 showed inhibition of 70.0%, while other compounds showed inhibition ranging from 19% to 36%.ConclusionOur study demonstrated that CMC 2.24 inhibited biodegradation of dental composite material more effectively than its mother compound, curcumin. Moreover, the mechanism of this biodegradation was likely mediated through bacterial esterase activity. Doxycycline achieved similar inhibition by completely eradicating S. mutans with its antibiotic action; hence, it is not recommended for long-term use.https://www.frontiersin.org/articles/10.3389/froh.2024.1506616/fulldental cariesdental cavitiesdental caries susceptibilityfluoridestreptococcus mutansdoxycycline |
| spellingShingle | Qi Dai Hsi-Ming Lee Austin Giordano Fu-Pen Chiang Stephen G. Walker Rafael Delgado-Ruiz Francis Johnson Lorne M. Golub Ying Gu Effect of a chemically-modified-curcumin on dental resin biodegradation Frontiers in Oral Health dental caries dental cavities dental caries susceptibility fluoride streptococcus mutans doxycycline |
| title | Effect of a chemically-modified-curcumin on dental resin biodegradation |
| title_full | Effect of a chemically-modified-curcumin on dental resin biodegradation |
| title_fullStr | Effect of a chemically-modified-curcumin on dental resin biodegradation |
| title_full_unstemmed | Effect of a chemically-modified-curcumin on dental resin biodegradation |
| title_short | Effect of a chemically-modified-curcumin on dental resin biodegradation |
| title_sort | effect of a chemically modified curcumin on dental resin biodegradation |
| topic | dental caries dental cavities dental caries susceptibility fluoride streptococcus mutans doxycycline |
| url | https://www.frontiersin.org/articles/10.3389/froh.2024.1506616/full |
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