Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface
Slightly acidic electrolyzed water (SAEW) has proven to be an efficient and novel sanitizer in food and agriculture field. This study assessed the efficacy of SAEW (30 mg/L) at 40 ℃ on the inactivation of foodborne pathogens and detachment of multi-resistant Staphylococcus aureus (MRSA) biofilm. Fur...
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Tsinghua University Press
2024-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250131 |
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| author | Pianpian Yan Ramachandran Chelliah Kyoung Hee Jo Xiuqin Chen Akanksha Tyagi Hyeon Yeong Jo Fazle Elahi Nam Chan Woo Min Seung Wook Deog Hwan Oh |
| author_facet | Pianpian Yan Ramachandran Chelliah Kyoung Hee Jo Xiuqin Chen Akanksha Tyagi Hyeon Yeong Jo Fazle Elahi Nam Chan Woo Min Seung Wook Deog Hwan Oh |
| author_sort | Pianpian Yan |
| collection | DOAJ |
| description | Slightly acidic electrolyzed water (SAEW) has proven to be an efficient and novel sanitizer in food and agriculture field. This study assessed the efficacy of SAEW (30 mg/L) at 40 ℃ on the inactivation of foodborne pathogens and detachment of multi-resistant Staphylococcus aureus (MRSA) biofilm. Furthermore, the underlying mechanism of MRSA biofilm under heated SAEW at 40 ℃ treatment on metabolic profiles was investigated. The results showed that the heated SAEW at 40 ℃ significantly effectively against foodborne pathogens of 1.96–7.56 (lg (CFU/g)) reduction in pork, chicken, spinach, and lettuce. The heated SAEW at 40 ℃ treatment significantly reduced MRSA biofilm cells by 2.41 (lg (CFU/cm2)). The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes. Global metabolic response of MRSA biofilms, treated by SAEW at 40 ℃, revealed the alterations of intracellular metabolites, including amino acids, organic acid, fatty acid, and lipid. Moreover, signaling pathways involved in amino acid metabolism, energy metabolism, nucleotide synthesis, carbohydrate metabolites, and lipid biosynthesis were functionally disrupted by the SAEW at 40 ℃ treatment. As per our knowledge, this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface. |
| format | Article |
| id | doaj-art-1c4fde718a6349f096a4114f5ed60b6a |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-1c4fde718a6349f096a4114f5ed60b6a2025-01-10T06:54:23ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-05-011331556156610.26599/FSHW.2022.9250131Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surfacePianpian Yan0Ramachandran Chelliah1Kyoung Hee Jo2Xiuqin Chen3Akanksha Tyagi4Hyeon Yeong Jo5Fazle Elahi6Nam Chan Woo7Min Seung Wook8Deog Hwan Oh9Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South KoreaSeoulin Bioscience Company, Seongnam-si 13488, South KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South KoreaSeoulin Bioscience Company, Seongnam-si 13488, South KoreaSeoulin Bioscience Company, Seongnam-si 13488, South KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South KoreaSlightly acidic electrolyzed water (SAEW) has proven to be an efficient and novel sanitizer in food and agriculture field. This study assessed the efficacy of SAEW (30 mg/L) at 40 ℃ on the inactivation of foodborne pathogens and detachment of multi-resistant Staphylococcus aureus (MRSA) biofilm. Furthermore, the underlying mechanism of MRSA biofilm under heated SAEW at 40 ℃ treatment on metabolic profiles was investigated. The results showed that the heated SAEW at 40 ℃ significantly effectively against foodborne pathogens of 1.96–7.56 (lg (CFU/g)) reduction in pork, chicken, spinach, and lettuce. The heated SAEW at 40 ℃ treatment significantly reduced MRSA biofilm cells by 2.41 (lg (CFU/cm2)). The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes. Global metabolic response of MRSA biofilms, treated by SAEW at 40 ℃, revealed the alterations of intracellular metabolites, including amino acids, organic acid, fatty acid, and lipid. Moreover, signaling pathways involved in amino acid metabolism, energy metabolism, nucleotide synthesis, carbohydrate metabolites, and lipid biosynthesis were functionally disrupted by the SAEW at 40 ℃ treatment. As per our knowledge, this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.https://www.sciopen.com/article/10.26599/FSHW.2022.9250131multi-resistant staphylococcus aureusmetabolic profilesaewbiofilmhurdle technologyelectrode material |
| spellingShingle | Pianpian Yan Ramachandran Chelliah Kyoung Hee Jo Xiuqin Chen Akanksha Tyagi Hyeon Yeong Jo Fazle Elahi Nam Chan Woo Min Seung Wook Deog Hwan Oh Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface Food Science and Human Wellness multi-resistant staphylococcus aureus metabolic profile saew biofilm hurdle technology electrode material |
| title | Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface |
| title_full | Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface |
| title_fullStr | Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface |
| title_full_unstemmed | Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface |
| title_short | Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface |
| title_sort | structural insights on anti biofilm mechanism of heated slightly acidic electrolyzed water technology against multi resistant staphylococcus aureus biofilm on food contact surface |
| topic | multi-resistant staphylococcus aureus metabolic profile saew biofilm hurdle technology electrode material |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250131 |
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