Enzymeless electrochemical detection of hydrogen peroxide using NiO octahedron decorated 3D graphene hydrogel
Abstract Nowadays, electrochemical biosensors have gained extensive consensus to detect a wide variety of analytes such as hydrogen peroxide (H2O2), glucose, dopamine, uric acid, and so on. However, the detection of H2O2 is more and more important because H2O2 plays a vital role in our daily life. H...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-10472-6 |
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| author | Mohamed A. Yassin Ayman F. Abou-Hadid Hamouda M. Mousa Chan Hee Park Cheol Sang Kim Ali Salem Mohamed A. Mattar |
| author_facet | Mohamed A. Yassin Ayman F. Abou-Hadid Hamouda M. Mousa Chan Hee Park Cheol Sang Kim Ali Salem Mohamed A. Mattar |
| author_sort | Mohamed A. Yassin |
| collection | DOAJ |
| description | Abstract Nowadays, electrochemical biosensors have gained extensive consensus to detect a wide variety of analytes such as hydrogen peroxide (H2O2), glucose, dopamine, uric acid, and so on. However, the detection of H2O2 is more and more important because H2O2 plays a vital role in our daily life. Hence, a new nonenzymatic H2O2 biosensor was developed by decorating NiO octahedrons on the 3-dimensional graphene hydrogel (3DGH). The NiO octahedrons were prepared using a mesoporous silica SBA-15 as a hard template. Then, different amounts of NiO octahedrons self-assembled with 3DGH during hydrothermal method. The performance of different prepared nanostructures as H2O2 biosensors electrodes was determined by the cyclic voltammetry and chronoamperometry tests. Various physicochemical methods such as FE-SEM, HR-TEM, XRD, TGA and Raman were utilized to characterize the morphology and structure properties of the as-prepared samples. The electrochemical sensing of H2O2 for the proposed biosensor electrodes are investigated, and the 3DGH/NiO nanocomposite electrode with NiO content of 25% displays high sensitivity with wide linear range and low detection limit, as well as good selectivity, reproducibility and long-term stability. Finally, the analytical utilities of the 3DGH/NiO25 proposed electrode were conducted to detect H2O2 in real products of milk samples. |
| format | Article |
| id | doaj-art-1c1e9e2e9781435d87a4c38f7b4f16ab |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
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| series | Scientific Reports |
| spelling | doaj-art-1c1e9e2e9781435d87a4c38f7b4f16ab2025-08-20T03:45:55ZengNature PortfolioScientific Reports2045-23222025-08-0115111410.1038/s41598-025-10472-6Enzymeless electrochemical detection of hydrogen peroxide using NiO octahedron decorated 3D graphene hydrogelMohamed A. Yassin0Ayman F. Abou-Hadid1Hamouda M. Mousa2Chan Hee Park3Cheol Sang Kim4Ali Salem5Mohamed A. Mattar6Department of Biosystems Engineering, Institute of Postgraduate Studies and Agricultural Research in Arid Regions, Ain Shams UniversityHorticulture Department, Faculty of Agriculture, Ain Shams UniversityDepartment of Mechanical Engineering, Faculty of Engineering, South Valley UniversityDepartment of Bionanosystem Engineering, Graduate School, Jeonbuk National UniversityDepartment of Bionanosystem Engineering, Graduate School, Jeonbuk National UniversityCivil Engineering Department, Faculty of Engineering, Minia UniversityPrince Sultan Bin Abdulaziz International Prize for Water Chair, Prince Sultan Institute for Environmental, Water and Desert Research, King Saud UniversityAbstract Nowadays, electrochemical biosensors have gained extensive consensus to detect a wide variety of analytes such as hydrogen peroxide (H2O2), glucose, dopamine, uric acid, and so on. However, the detection of H2O2 is more and more important because H2O2 plays a vital role in our daily life. Hence, a new nonenzymatic H2O2 biosensor was developed by decorating NiO octahedrons on the 3-dimensional graphene hydrogel (3DGH). The NiO octahedrons were prepared using a mesoporous silica SBA-15 as a hard template. Then, different amounts of NiO octahedrons self-assembled with 3DGH during hydrothermal method. The performance of different prepared nanostructures as H2O2 biosensors electrodes was determined by the cyclic voltammetry and chronoamperometry tests. Various physicochemical methods such as FE-SEM, HR-TEM, XRD, TGA and Raman were utilized to characterize the morphology and structure properties of the as-prepared samples. The electrochemical sensing of H2O2 for the proposed biosensor electrodes are investigated, and the 3DGH/NiO nanocomposite electrode with NiO content of 25% displays high sensitivity with wide linear range and low detection limit, as well as good selectivity, reproducibility and long-term stability. Finally, the analytical utilities of the 3DGH/NiO25 proposed electrode were conducted to detect H2O2 in real products of milk samples.https://doi.org/10.1038/s41598-025-10472-6Self-assemblyGraphene hydrogelOctahedronsElectrochemical biosensors |
| spellingShingle | Mohamed A. Yassin Ayman F. Abou-Hadid Hamouda M. Mousa Chan Hee Park Cheol Sang Kim Ali Salem Mohamed A. Mattar Enzymeless electrochemical detection of hydrogen peroxide using NiO octahedron decorated 3D graphene hydrogel Scientific Reports Self-assembly Graphene hydrogel Octahedrons Electrochemical biosensors |
| title | Enzymeless electrochemical detection of hydrogen peroxide using NiO octahedron decorated 3D graphene hydrogel |
| title_full | Enzymeless electrochemical detection of hydrogen peroxide using NiO octahedron decorated 3D graphene hydrogel |
| title_fullStr | Enzymeless electrochemical detection of hydrogen peroxide using NiO octahedron decorated 3D graphene hydrogel |
| title_full_unstemmed | Enzymeless electrochemical detection of hydrogen peroxide using NiO octahedron decorated 3D graphene hydrogel |
| title_short | Enzymeless electrochemical detection of hydrogen peroxide using NiO octahedron decorated 3D graphene hydrogel |
| title_sort | enzymeless electrochemical detection of hydrogen peroxide using nio octahedron decorated 3d graphene hydrogel |
| topic | Self-assembly Graphene hydrogel Octahedrons Electrochemical biosensors |
| url | https://doi.org/10.1038/s41598-025-10472-6 |
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