Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique
The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2020-06-01
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| Series: | Journal of Parasite Science |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JoPS/article/view/20176 |
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| author | Firman Hadi Fanani Kusnoto Kusnoto Poedji Hastutiek Setiawan Koesdarto Endang Suprihati |
| author_facet | Firman Hadi Fanani Kusnoto Kusnoto Poedji Hastutiek Setiawan Koesdarto Endang Suprihati |
| author_sort | Firman Hadi Fanani |
| collection | DOAJ |
| description | The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 μg/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa. |
| format | Article |
| id | doaj-art-1ba07e6692c74c0c80c9d88d967909e4 |
| institution | Kabale University |
| issn | 2599-0993 2656-5331 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Parasite Science |
| spelling | doaj-art-1ba07e6692c74c0c80c9d88d967909e42025-01-09T04:38:14ZengUniversitas AirlanggaJournal of Parasite Science2599-09932656-53312020-06-01411610.20473/jops.v4i1.2017616505Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot TechniqueFirman Hadi Fanani0Kusnoto Kusnoto1Poedji Hastutiek2Setiawan Koesdarto3Endang Suprihati4Faculty of Veterinary Medicine, Universitas AirlanggaDepartment of Veterinary Parasitology, Faculty of Veterinary Parasitology, Universitas Airlangga, SurabayaDepartment of Veterinary Parasitology, Faculty of Veterinary Parasitology, Universitas Airlangga, SurabayaDepartment of Veterinary Parasitology, Faculty of Veterinary Parasitology, Universitas Airlangga, SurabayaDepartment of Veterinary Parasitology, Faculty of Veterinary Parasitology, Universitas Airlangga, SurabayaThe purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 μg/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa.https://e-journal.unair.ac.id/JoPS/article/view/20176haemonchus contortusfasciola giganticacross reactionwesternblot |
| spellingShingle | Firman Hadi Fanani Kusnoto Kusnoto Poedji Hastutiek Setiawan Koesdarto Endang Suprihati Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique Journal of Parasite Science haemonchus contortus fasciola gigantica cross reaction westernblot |
| title | Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique |
| title_full | Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique |
| title_fullStr | Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique |
| title_full_unstemmed | Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique |
| title_short | Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique |
| title_sort | cross reaction of haemonchus contortus antigen with anti fasciola gigantica serum by using western blot technique |
| topic | haemonchus contortus fasciola gigantica cross reaction westernblot |
| url | https://e-journal.unair.ac.id/JoPS/article/view/20176 |
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