Insights into host-pathogen interaction based on the comparison of genomes of Leptospira interrogans isolated from dogs, humans, and a rodent in the same epidemiological context: A one health approach
Leptospirosis is a zoonotic infectious disease that significantly impacts animal and public health. Comparative genomics can aid in understanding poorly understood aspects of leptospirosis pathogenesis, including infection mechanisms, antimicrobial resistance, and host interactions across different...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024175627 |
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| author | Lucas Nogueira Paz Laise de Moraes Luciane Amorim Santos Camila Hamond Melissa Hanzen Pinna |
| author_facet | Lucas Nogueira Paz Laise de Moraes Luciane Amorim Santos Camila Hamond Melissa Hanzen Pinna |
| author_sort | Lucas Nogueira Paz |
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| description | Leptospirosis is a zoonotic infectious disease that significantly impacts animal and public health. Comparative genomics can aid in understanding poorly understood aspects of leptospirosis pathogenesis, including infection mechanisms, antimicrobial resistance, and host interactions across different epidemiological scenarios. This study aimed to compare the genomes of Leptospira interrogans serogroup Icterohaemorrhagiae strains isolated from three host species in a single epidemiological scenario. Four strains of L. interrogans serogroup Icterohaemorrhagiae from naturally infected and clinically symptomatic dogs (C20, C29, C51, and C82) were processed for whole genome sequencing (WGS). These results were compared against WGS data from two other rodent and human strains. Phylogenetic and genomic similarity analyses demonstrated high identity and synteny between the strains isolated from humans, canines, and rodents. Small regions of divergence were observed, especially in the genome obtained from a rodent sample. The presence of 23 genes potentially associated with biofilm formation was notable, with the identification of missense mutations in eight genes. Considering the need to better understand the molecular basis involved in biofilm formation, it is of fundamental importance to elucidate the effect of mutations on the expression of the phenotype (biofilm) among different strains. The present findings highlight the necessity of One Health-based collaborative interventions to address the complex dynamics of leptospirosis transmission, involving both common hosts such as rodents and dogs, as well as less-recognized hosts. |
| format | Article |
| id | doaj-art-1b98f4d95b034c5b8905e0d39a411e10 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| spelling | doaj-art-1b98f4d95b034c5b8905e0d39a411e102025-01-17T04:51:33ZengElsevierHeliyon2405-84402025-01-01111e41531Insights into host-pathogen interaction based on the comparison of genomes of Leptospira interrogans isolated from dogs, humans, and a rodent in the same epidemiological context: A one health approachLucas Nogueira Paz0Laise de Moraes1Luciane Amorim Santos2Camila Hamond3Melissa Hanzen Pinna4Bacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics – Federal University of Bahia, Salvador, Bahia, BrazilPrograma de Pós-graduação em Ciências da Saúde, Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Bahia, Brazil; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, BrazilPrograma de Pós-graduação em Ciências da Saúde, Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Bahia, Brazil; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Escola Bahiana de Medicina e Saúde Pública, Salvador, Bahia, BrazilNational Veterinary Services Laboratories, Animal and Plant Health Inspection Service, US Department of Agriculture, Ames, IA, USA; Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose, WHO/PAHO Centro Colaborador para Leptospirose, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, BrazilBacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics – Federal University of Bahia, Salvador, Bahia, Brazil; Corresponding author. Department of Preventive Veterinary Medicine and Animal Production, Universidade Federal da Bahia, Av. Milton Santos, 500, Ondina, CEP, Salvador, Bahia, 40170-110, Brazil.Leptospirosis is a zoonotic infectious disease that significantly impacts animal and public health. Comparative genomics can aid in understanding poorly understood aspects of leptospirosis pathogenesis, including infection mechanisms, antimicrobial resistance, and host interactions across different epidemiological scenarios. This study aimed to compare the genomes of Leptospira interrogans serogroup Icterohaemorrhagiae strains isolated from three host species in a single epidemiological scenario. Four strains of L. interrogans serogroup Icterohaemorrhagiae from naturally infected and clinically symptomatic dogs (C20, C29, C51, and C82) were processed for whole genome sequencing (WGS). These results were compared against WGS data from two other rodent and human strains. Phylogenetic and genomic similarity analyses demonstrated high identity and synteny between the strains isolated from humans, canines, and rodents. Small regions of divergence were observed, especially in the genome obtained from a rodent sample. The presence of 23 genes potentially associated with biofilm formation was notable, with the identification of missense mutations in eight genes. Considering the need to better understand the molecular basis involved in biofilm formation, it is of fundamental importance to elucidate the effect of mutations on the expression of the phenotype (biofilm) among different strains. The present findings highlight the necessity of One Health-based collaborative interventions to address the complex dynamics of leptospirosis transmission, involving both common hosts such as rodents and dogs, as well as less-recognized hosts.http://www.sciencedirect.com/science/article/pii/S2405844024175627Comparative genomic analysisLeptospira interrogansLeptospirosisWhole genome sequencingOne health |
| spellingShingle | Lucas Nogueira Paz Laise de Moraes Luciane Amorim Santos Camila Hamond Melissa Hanzen Pinna Insights into host-pathogen interaction based on the comparison of genomes of Leptospira interrogans isolated from dogs, humans, and a rodent in the same epidemiological context: A one health approach Heliyon Comparative genomic analysis Leptospira interrogans Leptospirosis Whole genome sequencing One health |
| title | Insights into host-pathogen interaction based on the comparison of genomes of Leptospira interrogans isolated from dogs, humans, and a rodent in the same epidemiological context: A one health approach |
| title_full | Insights into host-pathogen interaction based on the comparison of genomes of Leptospira interrogans isolated from dogs, humans, and a rodent in the same epidemiological context: A one health approach |
| title_fullStr | Insights into host-pathogen interaction based on the comparison of genomes of Leptospira interrogans isolated from dogs, humans, and a rodent in the same epidemiological context: A one health approach |
| title_full_unstemmed | Insights into host-pathogen interaction based on the comparison of genomes of Leptospira interrogans isolated from dogs, humans, and a rodent in the same epidemiological context: A one health approach |
| title_short | Insights into host-pathogen interaction based on the comparison of genomes of Leptospira interrogans isolated from dogs, humans, and a rodent in the same epidemiological context: A one health approach |
| title_sort | insights into host pathogen interaction based on the comparison of genomes of leptospira interrogans isolated from dogs humans and a rodent in the same epidemiological context a one health approach |
| topic | Comparative genomic analysis Leptospira interrogans Leptospirosis Whole genome sequencing One health |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024175627 |
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