3D bioprinted chondrogenic gelatin methacrylate-poly(ethylene glycol) diacrylate composite scaffolds for intervertebral disc restoration
Degenerative spine pathologies, including intervertebral disc (IVD) degeneration, present a significant healthcare challenge due to their association with chronic pain and disability. This study explores an innovative approach to IVD regeneration utilizing 3D bioprinting technology, specifically vis...
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| Format: | Article |
| Language: | English |
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IOP Publishing
2024-01-01
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| Series: | International Journal of Extreme Manufacturing |
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| Online Access: | https://doi.org/10.1088/2631-7990/ad878e |
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| author | Maria D Astudillo Potes Maryam Tilton Indranath Mitra Xifeng Liu Babak Dashtdar Emily T Camilleri Benjamin D Elder Lichun Lu |
| author_facet | Maria D Astudillo Potes Maryam Tilton Indranath Mitra Xifeng Liu Babak Dashtdar Emily T Camilleri Benjamin D Elder Lichun Lu |
| author_sort | Maria D Astudillo Potes |
| collection | DOAJ |
| description | Degenerative spine pathologies, including intervertebral disc (IVD) degeneration, present a significant healthcare challenge due to their association with chronic pain and disability. This study explores an innovative approach to IVD regeneration utilizing 3D bioprinting technology, specifically visible light-based digital light processing, to fabricate tissue scaffolds that closely mimic the native architecture of the IVD. Utilizing a hybrid bioink composed of gelatin methacrylate (GelMA) and poly (ethylene glycol) diacrylate (PEGDA) at a 10% concentration, we achieved enhanced printing fidelity and mechanical properties suitable for load-bearing applications such as the IVD. Preconditioning rat bone marrow-derived mesenchymal stem cell spheroids with chondrogenic media before incorporating them into the GelMA-PEGDA scaffold further promoted the regenerative capabilities of this system. Our findings demonstrate that this bioprinted scaffold not only supports cell viability and integration but also contributes to the restoration of disc height in a rat caudal disc model without inducing adverse inflammatory responses. The study underscores the potential of combining advanced bioprinting techniques and cell preconditioning strategies to develop effective treatments for IVD degeneration and other musculoskeletal disorders, highlighting the need for further research into the dynamic interplay between cellular migration and the hydrogel matrix. |
| format | Article |
| id | doaj-art-1b6908b0e1ce4d349d449b4d0a3e99b2 |
| institution | Kabale University |
| issn | 2631-7990 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | International Journal of Extreme Manufacturing |
| spelling | doaj-art-1b6908b0e1ce4d349d449b4d0a3e99b22024-11-19T12:13:15ZengIOP PublishingInternational Journal of Extreme Manufacturing2631-79902024-01-017101550710.1088/2631-7990/ad878e3D bioprinted chondrogenic gelatin methacrylate-poly(ethylene glycol) diacrylate composite scaffolds for intervertebral disc restorationMaria D Astudillo Potes0https://orcid.org/0000-0001-8236-0808Maryam Tilton1Indranath Mitra2Xifeng Liu3Babak Dashtdar4Emily T Camilleri5Benjamin D Elder6Lichun Lu7Mayo Clinic Alix School of Medicine , Rochester, MN, United States of America; Mayo Clinic Graduate School of Biomedical Sciences , Rochester, MN, United States of America; Department of Physiology and Biomedical Engineering, Mayo Clinic , Rochester, MN, United States of America; Department of Orthopedic Surgery, Mayo Clinic , Rochester, MN, United States of America; Department of Neurological Surgery, Mayo Clinic , Rochester, MN, United States of AmericaWalker Department of Mechanical Engineering, The University of Texas at Austin , Austin, TX, United States of AmericaDepartment of Physiology and Biomedical Engineering, Mayo Clinic , Rochester, MN, United States of America; Department of Orthopedic Surgery, Mayo Clinic , Rochester, MN, United States of AmericaDepartment of Physiology and Biomedical Engineering, Mayo Clinic , Rochester, MN, United States of America; Department of Orthopedic Surgery, Mayo Clinic , Rochester, MN, United States of AmericaDepartment of Physiology and Biomedical Engineering, Mayo Clinic , Rochester, MN, United States of America; Department of Orthopedic Surgery, Mayo Clinic , Rochester, MN, United States of AmericaDepartment of Physiology and Biomedical Engineering, Mayo Clinic , Rochester, MN, United States of America; Department of Orthopedic Surgery, Mayo Clinic , Rochester, MN, United States of AmericaDepartment of Orthopedic Surgery, Mayo Clinic , Rochester, MN, United States of America; Department of Neurological Surgery, Mayo Clinic , Rochester, MN, United States of AmericaDepartment of Physiology and Biomedical Engineering, Mayo Clinic , Rochester, MN, United States of America; Department of Orthopedic Surgery, Mayo Clinic , Rochester, MN, United States of AmericaDegenerative spine pathologies, including intervertebral disc (IVD) degeneration, present a significant healthcare challenge due to their association with chronic pain and disability. This study explores an innovative approach to IVD regeneration utilizing 3D bioprinting technology, specifically visible light-based digital light processing, to fabricate tissue scaffolds that closely mimic the native architecture of the IVD. Utilizing a hybrid bioink composed of gelatin methacrylate (GelMA) and poly (ethylene glycol) diacrylate (PEGDA) at a 10% concentration, we achieved enhanced printing fidelity and mechanical properties suitable for load-bearing applications such as the IVD. Preconditioning rat bone marrow-derived mesenchymal stem cell spheroids with chondrogenic media before incorporating them into the GelMA-PEGDA scaffold further promoted the regenerative capabilities of this system. Our findings demonstrate that this bioprinted scaffold not only supports cell viability and integration but also contributes to the restoration of disc height in a rat caudal disc model without inducing adverse inflammatory responses. The study underscores the potential of combining advanced bioprinting techniques and cell preconditioning strategies to develop effective treatments for IVD degeneration and other musculoskeletal disorders, highlighting the need for further research into the dynamic interplay between cellular migration and the hydrogel matrix.https://doi.org/10.1088/2631-7990/ad878eintervertebral disc regeneration3D bioprintinggelatin-based hydrogelsmesenchymal stem cell spheroidstissue engineering |
| spellingShingle | Maria D Astudillo Potes Maryam Tilton Indranath Mitra Xifeng Liu Babak Dashtdar Emily T Camilleri Benjamin D Elder Lichun Lu 3D bioprinted chondrogenic gelatin methacrylate-poly(ethylene glycol) diacrylate composite scaffolds for intervertebral disc restoration International Journal of Extreme Manufacturing intervertebral disc regeneration 3D bioprinting gelatin-based hydrogels mesenchymal stem cell spheroids tissue engineering |
| title | 3D bioprinted chondrogenic gelatin methacrylate-poly(ethylene glycol) diacrylate composite scaffolds for intervertebral disc restoration |
| title_full | 3D bioprinted chondrogenic gelatin methacrylate-poly(ethylene glycol) diacrylate composite scaffolds for intervertebral disc restoration |
| title_fullStr | 3D bioprinted chondrogenic gelatin methacrylate-poly(ethylene glycol) diacrylate composite scaffolds for intervertebral disc restoration |
| title_full_unstemmed | 3D bioprinted chondrogenic gelatin methacrylate-poly(ethylene glycol) diacrylate composite scaffolds for intervertebral disc restoration |
| title_short | 3D bioprinted chondrogenic gelatin methacrylate-poly(ethylene glycol) diacrylate composite scaffolds for intervertebral disc restoration |
| title_sort | 3d bioprinted chondrogenic gelatin methacrylate poly ethylene glycol diacrylate composite scaffolds for intervertebral disc restoration |
| topic | intervertebral disc regeneration 3D bioprinting gelatin-based hydrogels mesenchymal stem cell spheroids tissue engineering |
| url | https://doi.org/10.1088/2631-7990/ad878e |
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