Antimicrobial activity against some human pathogenic microorganisms of silk spiders; Agelena labyrinthica (Agelenidae) and Holochnemus pluchei (Pholcidae)

Nowadays, where the use of biological materials is increasing, the antimicrobial activities of ethanol, methanol, and acetone extracts of silk from the species Agelena labyrinthica (Clerck, 1757) (Agelenidae) and the acetone extract of silk from the species Holocnemus pluchei (Scopoli, 1763) (Pholci...

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Main Authors: Bayda Hameed, Derya İşler Ceyhan, Adile Akpınar
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Natural Fibers
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Online Access:https://www.tandfonline.com/doi/10.1080/15440478.2024.2439552
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author Bayda Hameed
Derya İşler Ceyhan
Adile Akpınar
author_facet Bayda Hameed
Derya İşler Ceyhan
Adile Akpınar
author_sort Bayda Hameed
collection DOAJ
description Nowadays, where the use of biological materials is increasing, the antimicrobial activities of ethanol, methanol, and acetone extracts of silk from the species Agelena labyrinthica (Clerck, 1757) (Agelenidae) and the acetone extract of silk from the species Holocnemus pluchei (Scopoli, 1763) (Pholcidae), which produces insufficient silk, have been studied. Extracts from both spider species were tested against three Gram-negative bacteria (Pseudomonas aeruginosa (ATCC 27,853), Klebsiella pneumonia (ATCC 700,603), and Escherichia coli (ATCC 25,922)), one Gram-positive bacteria (Staphylococcus aureus (ATCC 25,923)), and one yeast (Candida albicans (ATCC 90,028)). It was determined that the inhibition zone diameters created by H. pluchei silk acetone extract against E. coli, P. aeruginosa, and K. pneumonia during 24-h incubation period (14 ± 0.1 mm, 11 ± 0.2 mm, 6.5 ± 0.5 mm, respectively) were greater than those created by A. labyrinthica acetone extract (12 ± 0.2 mm, 7 ± 0.0 mm, 6 ± 0.1 mm, respectively). However, it was found that H. pluchei silk acetone, A. labyrinthica ethanol, and methanol extracts had no antimicrobial activity against S. aureus, but A. labyrinthica silk acetone extract created an inhibition zone diameter of 9.5 ± 0.5 mm. Accordingly, it was determined that the antimicrobial activities of spider silks varied depending on the spider species and the solvent used to obtain the silk extract.
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spelling doaj-art-1b6436b99a6b48059fca5b5951eaa8812024-12-21T14:30:24ZengTaylor & Francis GroupJournal of Natural Fibers1544-04781544-046X2025-12-0122110.1080/15440478.2024.2439552Antimicrobial activity against some human pathogenic microorganisms of silk spiders; Agelena labyrinthica (Agelenidae) and Holochnemus pluchei (Pholcidae)Bayda Hameed0Derya İşler Ceyhan1Adile Akpınar2Faculty of Arts and Sciences, Gazi University, Gaziantep University, Ankara, TurkeyFaculty of Arts and Sciences, Department of Biology, Gaziantep University, Gaziantep, TurkeyFaculty of Arts and Sciences, Department of Biology, Gaziantep University, Gaziantep, TurkeyNowadays, where the use of biological materials is increasing, the antimicrobial activities of ethanol, methanol, and acetone extracts of silk from the species Agelena labyrinthica (Clerck, 1757) (Agelenidae) and the acetone extract of silk from the species Holocnemus pluchei (Scopoli, 1763) (Pholcidae), which produces insufficient silk, have been studied. Extracts from both spider species were tested against three Gram-negative bacteria (Pseudomonas aeruginosa (ATCC 27,853), Klebsiella pneumonia (ATCC 700,603), and Escherichia coli (ATCC 25,922)), one Gram-positive bacteria (Staphylococcus aureus (ATCC 25,923)), and one yeast (Candida albicans (ATCC 90,028)). It was determined that the inhibition zone diameters created by H. pluchei silk acetone extract against E. coli, P. aeruginosa, and K. pneumonia during 24-h incubation period (14 ± 0.1 mm, 11 ± 0.2 mm, 6.5 ± 0.5 mm, respectively) were greater than those created by A. labyrinthica acetone extract (12 ± 0.2 mm, 7 ± 0.0 mm, 6 ± 0.1 mm, respectively). However, it was found that H. pluchei silk acetone, A. labyrinthica ethanol, and methanol extracts had no antimicrobial activity against S. aureus, but A. labyrinthica silk acetone extract created an inhibition zone diameter of 9.5 ± 0.5 mm. Accordingly, it was determined that the antimicrobial activities of spider silks varied depending on the spider species and the solvent used to obtain the silk extract.https://www.tandfonline.com/doi/10.1080/15440478.2024.2439552Antimicrobial activityspiderspider silkpathogenic microorganismsAgelena labyrinthicaHolocnemus pluch
spellingShingle Bayda Hameed
Derya İşler Ceyhan
Adile Akpınar
Antimicrobial activity against some human pathogenic microorganisms of silk spiders; Agelena labyrinthica (Agelenidae) and Holochnemus pluchei (Pholcidae)
Journal of Natural Fibers
Antimicrobial activity
spider
spider silk
pathogenic microorganisms
Agelena labyrinthica
Holocnemus pluch
title Antimicrobial activity against some human pathogenic microorganisms of silk spiders; Agelena labyrinthica (Agelenidae) and Holochnemus pluchei (Pholcidae)
title_full Antimicrobial activity against some human pathogenic microorganisms of silk spiders; Agelena labyrinthica (Agelenidae) and Holochnemus pluchei (Pholcidae)
title_fullStr Antimicrobial activity against some human pathogenic microorganisms of silk spiders; Agelena labyrinthica (Agelenidae) and Holochnemus pluchei (Pholcidae)
title_full_unstemmed Antimicrobial activity against some human pathogenic microorganisms of silk spiders; Agelena labyrinthica (Agelenidae) and Holochnemus pluchei (Pholcidae)
title_short Antimicrobial activity against some human pathogenic microorganisms of silk spiders; Agelena labyrinthica (Agelenidae) and Holochnemus pluchei (Pholcidae)
title_sort antimicrobial activity against some human pathogenic microorganisms of silk spiders agelena labyrinthica agelenidae and holochnemus pluchei pholcidae
topic Antimicrobial activity
spider
spider silk
pathogenic microorganisms
Agelena labyrinthica
Holocnemus pluch
url https://www.tandfonline.com/doi/10.1080/15440478.2024.2439552
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AT deryaislerceyhan antimicrobialactivityagainstsomehumanpathogenicmicroorganismsofsilkspidersagelenalabyrinthicaagelenidaeandholochnemusplucheipholcidae
AT adileakpınar antimicrobialactivityagainstsomehumanpathogenicmicroorganismsofsilkspidersagelenalabyrinthicaagelenidaeandholochnemusplucheipholcidae