Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil
<i>Wolbachia</i> are endosymbiotic bacteria that infect a wide range of arthropods and filarial nematodes, often manipulating host reproduction. The efficacy of <i>Wolbachia</i>-based interventions for dengue and chikungunya control has been validated through numerous field s...
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MDPI AG
2024-10-01
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| Online Access: | https://www.mdpi.com/2076-2607/12/11/2206 |
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| author | Luísa Maria Inácio da Silva José Irnaldo da Silva Alexandre Freitas da Silva Filipe Zimmer Dezordi Lais Ceschini Machado Si Qin Hang Fan Yigang Tong Túlio de Lima Campos Marcelo Henrique Santos Paiva Gabriel Luz Wallau |
| author_facet | Luísa Maria Inácio da Silva José Irnaldo da Silva Alexandre Freitas da Silva Filipe Zimmer Dezordi Lais Ceschini Machado Si Qin Hang Fan Yigang Tong Túlio de Lima Campos Marcelo Henrique Santos Paiva Gabriel Luz Wallau |
| author_sort | Luísa Maria Inácio da Silva |
| collection | DOAJ |
| description | <i>Wolbachia</i> are endosymbiotic bacteria that infect a wide range of arthropods and filarial nematodes, often manipulating host reproduction. The efficacy of <i>Wolbachia</i>-based interventions for dengue and chikungunya control has been validated through numerous field studies in recent years. This study aimed to investigate the diversity and prevalence of <i>Wolbachia</i> infections in sylvatic mosquitoes from two locations in Recife, Brazil. Multiple mosquito species were screened for <i>Wolbachia</i> using both target marker gene amplification coupled with Sanger sequencing and whole-genome sequencing (WGS) approaches. Phylogenetic analyses were conducted to classify <i>Wolbachia</i> strains into supergroups and assess their evolutionary relationships. Results revealed the presence of <i>Wolbachia</i> in eleven mosquito species examined, with different infection rates. Both supergroups A and B of <i>Wolbachia</i> strains were identified, with <i>Aedes albopictus</i> showing co-infection by both supergroups through the WGS approach. We also detected indirect evidence of <i>Wolbachia</i> horizontal transmission among mosquitoes and other distant host orders. This study provides valuable insights into the distribution and diversity of <i>Wolbachia</i> in sylvatic mosquitoes from Brazil and adds new important data about <i>Wolbachia</i> detection through target marker gene amplicon coupled with Sanger sequencing and WGS methods, highlighting its complementarity to ascertain the presence of <i>Wolbachia</i> in mosquito samples. |
| format | Article |
| id | doaj-art-1b4e1d7ddf1e4d6e8f3748d7b0361796 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-1b4e1d7ddf1e4d6e8f3748d7b03617962024-11-26T18:14:30ZengMDPI AGMicroorganisms2076-26072024-10-011211220610.3390/microorganisms12112206Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from BrazilLuísa Maria Inácio da Silva0José Irnaldo da Silva1Alexandre Freitas da Silva2Filipe Zimmer Dezordi3Lais Ceschini Machado4Si Qin5Hang Fan6Yigang Tong7Túlio de Lima Campos8Marcelo Henrique Santos Paiva9Gabriel Luz Wallau10Departamento de Entomologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 21040-900, PE, BrazilDepartamento de Entomologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 21040-900, PE, BrazilDepartamento de Entomologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 21040-900, PE, BrazilDepartamento de Entomologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 21040-900, PE, BrazilDepartamento de Entomologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 21040-900, PE, BrazilState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaBeijing Advanced Innovation Center for Soft Matter Science and Engineering (BAIC-SM), College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaNúcleo de Bioinformática e Departamento de Entomologia, Fundação Oswaldo Cruz, Recife 21040-900, PE, BrazilDepartamento de Entomologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 21040-900, PE, BrazilDepartamento de Entomologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 21040-900, PE, Brazil<i>Wolbachia</i> are endosymbiotic bacteria that infect a wide range of arthropods and filarial nematodes, often manipulating host reproduction. The efficacy of <i>Wolbachia</i>-based interventions for dengue and chikungunya control has been validated through numerous field studies in recent years. This study aimed to investigate the diversity and prevalence of <i>Wolbachia</i> infections in sylvatic mosquitoes from two locations in Recife, Brazil. Multiple mosquito species were screened for <i>Wolbachia</i> using both target marker gene amplification coupled with Sanger sequencing and whole-genome sequencing (WGS) approaches. Phylogenetic analyses were conducted to classify <i>Wolbachia</i> strains into supergroups and assess their evolutionary relationships. Results revealed the presence of <i>Wolbachia</i> in eleven mosquito species examined, with different infection rates. Both supergroups A and B of <i>Wolbachia</i> strains were identified, with <i>Aedes albopictus</i> showing co-infection by both supergroups through the WGS approach. We also detected indirect evidence of <i>Wolbachia</i> horizontal transmission among mosquitoes and other distant host orders. This study provides valuable insights into the distribution and diversity of <i>Wolbachia</i> in sylvatic mosquitoes from Brazil and adds new important data about <i>Wolbachia</i> detection through target marker gene amplicon coupled with Sanger sequencing and WGS methods, highlighting its complementarity to ascertain the presence of <i>Wolbachia</i> in mosquito samples.https://www.mdpi.com/2076-2607/12/11/2206genomicssymbiontshorizontal transfer |
| spellingShingle | Luísa Maria Inácio da Silva José Irnaldo da Silva Alexandre Freitas da Silva Filipe Zimmer Dezordi Lais Ceschini Machado Si Qin Hang Fan Yigang Tong Túlio de Lima Campos Marcelo Henrique Santos Paiva Gabriel Luz Wallau Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil Microorganisms genomics symbionts horizontal transfer |
| title | Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil |
| title_full | Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil |
| title_fullStr | Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil |
| title_full_unstemmed | Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil |
| title_short | Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil |
| title_sort | sequencing and analysis of i wolbachia i strains from a and b supergroups detected in sylvatic mosquitoes from brazil |
| topic | genomics symbionts horizontal transfer |
| url | https://www.mdpi.com/2076-2607/12/11/2206 |
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