Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil

Sequencing and Analysis of <i>Wolbachia</i> Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil

<i>Wolbachia</i> are endosymbiotic bacteria that infect a wide range of arthropods and filarial nematodes, often manipulating host reproduction. The efficacy of <i>Wolbachia</i>-based interventions for dengue and chikungunya control has been validated through numerous field s...

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Main Authors: Luísa Maria Inácio da Silva, José Irnaldo da Silva, Alexandre Freitas da Silva, Filipe Zimmer Dezordi, Lais Ceschini Machado, Si Qin, Hang Fan, Yigang Tong, Túlio de Lima Campos, Marcelo Henrique Santos Paiva, Gabriel Luz Wallau
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Microorganisms
Subjects:
genomics
symbionts
horizontal transfer
Online Access:https://www.mdpi.com/2076-2607/12/11/2206
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Summary:<i>Wolbachia</i> are endosymbiotic bacteria that infect a wide range of arthropods and filarial nematodes, often manipulating host reproduction. The efficacy of <i>Wolbachia</i>-based interventions for dengue and chikungunya control has been validated through numerous field studies in recent years. This study aimed to investigate the diversity and prevalence of <i>Wolbachia</i> infections in sylvatic mosquitoes from two locations in Recife, Brazil. Multiple mosquito species were screened for <i>Wolbachia</i> using both target marker gene amplification coupled with Sanger sequencing and whole-genome sequencing (WGS) approaches. Phylogenetic analyses were conducted to classify <i>Wolbachia</i> strains into supergroups and assess their evolutionary relationships. Results revealed the presence of <i>Wolbachia</i> in eleven mosquito species examined, with different infection rates. Both supergroups A and B of <i>Wolbachia</i> strains were identified, with <i>Aedes albopictus</i> showing co-infection by both supergroups through the WGS approach. We also detected indirect evidence of <i>Wolbachia</i> horizontal transmission among mosquitoes and other distant host orders. This study provides valuable insights into the distribution and diversity of <i>Wolbachia</i> in sylvatic mosquitoes from Brazil and adds new important data about <i>Wolbachia</i> detection through target marker gene amplicon coupled with Sanger sequencing and WGS methods, highlighting its complementarity to ascertain the presence of <i>Wolbachia</i> in mosquito samples.
ISSN:2076-2607

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