Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion
The bioconversion of lignocellulose has attracted global attention, due to the significant potential of agricultural and forestry wastes as renewable zero-carbon resources and the urgent need for substituting fossil carbon. The cellulosome system is a multi-enzyme complex produced by anaerobic bacte...
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KeAi Communications Co., Ltd.
2024-03-01
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| Series: | Green Carbon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950155524000090 |
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| author | Min Xiao Ya-Jun Liu Edward A. Bayer Akihiko Kosugi Qiu Cui Yingang Feng |
| author_facet | Min Xiao Ya-Jun Liu Edward A. Bayer Akihiko Kosugi Qiu Cui Yingang Feng |
| author_sort | Min Xiao |
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| description | The bioconversion of lignocellulose has attracted global attention, due to the significant potential of agricultural and forestry wastes as renewable zero-carbon resources and the urgent need for substituting fossil carbon. The cellulosome system is a multi-enzyme complex produced by anaerobic bacteria, which comprises cellulases, hemicellulases, and associated enzymatic and non-enzymatic components that promote biomass conversion. To enhance their efficiency in degrading recalcitrant lignocellulosic matrices, cellulosomes have been employed to construct biocatalysts for lignocellulose bioconversion, such as consolidated bioprocessing and consolidated bio-saccharification. Hemicelluloses, the second most abundant polysaccharides in plant cell walls, hold valuable application potential but can also induce inhibitory effects on cellulose hydrolysis, thus highlighting the indispensable roles of hemicellulases within the cellulosome complex. This review evaluated current research on cellulosomal hemicellulases, comparing their types, abundance, and regulation, primarily focusing on eight known cellulosome-producing species of different origins. We also reviewed their growth conditions, their hemicellulose-degrading capabilities, and the inhibitory effects of hemicellulose on cellulosome-based lignocellulose saccharification. Finally, we proposed strategies for targeted enhancement of hemicellulase in cellulosomes to improve lignocellulose bioconversion in future studies. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-03-01 |
| publisher | KeAi Communications Co., Ltd. |
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| spelling | doaj-art-1b40c21dcdfd448c81a2abb1d51c55b02025-08-20T03:42:43ZengKeAi Communications Co., Ltd.Green Carbon2950-15552024-03-0121576910.1016/j.greenca.2024.01.003Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversionMin Xiao0Ya-Jun Liu1Edward A. Bayer2Akihiko Kosugi3Qiu Cui4Yingang Feng5CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Energy Institute, Qingdao 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Energy Institute, Qingdao 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authorDepartment of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot 7610001, Israel; Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 8499000, IsraelBiological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1–1 Ohwashi, Tsukuba, Ibaraki 305–8686, JapanCAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Energy Institute, Qingdao 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Shandong Energy Institute, Qingdao 266101, China; Qingdao New Energy Shandong Laboratory, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaThe bioconversion of lignocellulose has attracted global attention, due to the significant potential of agricultural and forestry wastes as renewable zero-carbon resources and the urgent need for substituting fossil carbon. The cellulosome system is a multi-enzyme complex produced by anaerobic bacteria, which comprises cellulases, hemicellulases, and associated enzymatic and non-enzymatic components that promote biomass conversion. To enhance their efficiency in degrading recalcitrant lignocellulosic matrices, cellulosomes have been employed to construct biocatalysts for lignocellulose bioconversion, such as consolidated bioprocessing and consolidated bio-saccharification. Hemicelluloses, the second most abundant polysaccharides in plant cell walls, hold valuable application potential but can also induce inhibitory effects on cellulose hydrolysis, thus highlighting the indispensable roles of hemicellulases within the cellulosome complex. This review evaluated current research on cellulosomal hemicellulases, comparing their types, abundance, and regulation, primarily focusing on eight known cellulosome-producing species of different origins. We also reviewed their growth conditions, their hemicellulose-degrading capabilities, and the inhibitory effects of hemicellulose on cellulosome-based lignocellulose saccharification. Finally, we proposed strategies for targeted enhancement of hemicellulase in cellulosomes to improve lignocellulose bioconversion in future studies.http://www.sciencedirect.com/science/article/pii/S2950155524000090CellulosomeLignocelluloseHemicellulaseSaccharificationBiocatalyst |
| spellingShingle | Min Xiao Ya-Jun Liu Edward A. Bayer Akihiko Kosugi Qiu Cui Yingang Feng Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion Green Carbon Cellulosome Lignocellulose Hemicellulase Saccharification Biocatalyst |
| title | Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion |
| title_full | Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion |
| title_fullStr | Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion |
| title_full_unstemmed | Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion |
| title_short | Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion |
| title_sort | cellulosomal hemicellulases indispensable players for ensuring effective lignocellulose bioconversion |
| topic | Cellulosome Lignocellulose Hemicellulase Saccharification Biocatalyst |
| url | http://www.sciencedirect.com/science/article/pii/S2950155524000090 |
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