Impact of antibiotic prophylaxis on gut microbiota in colorectal surgery: insights from an Eastern European stewardship study
IntroductionAntibiotic overuse is driving a global rise in antibiotic resistance, highlighting the need for robust antimicrobial stewardship (AMS) initiatives to improve prescription practices. While antimicrobials are essential for treating sepsis and preventing surgical site infections (SSIs), the...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| author | Irina Cezara Văcărean-Trandafir Roxana-Maria Amărandi Iuliu Cristian Ivanov Loredana Mihaiela Dragoș Mihaela Mențel Ştefan Iacob Ştefan Iacob Ana-Maria Muşină Ana-Maria Muşină Elena-Roxana Bărgăoanu Cristian Ene Roată Cristian Ene Roată Ștefan Morărașu Ștefan Morărașu Valeri Țuțuianu Marcel Ciobanu Mihail-Gabriel Dimofte Mihail-Gabriel Dimofte |
| author_facet | Irina Cezara Văcărean-Trandafir Roxana-Maria Amărandi Iuliu Cristian Ivanov Loredana Mihaiela Dragoș Mihaela Mențel Ştefan Iacob Ştefan Iacob Ana-Maria Muşină Ana-Maria Muşină Elena-Roxana Bărgăoanu Cristian Ene Roată Cristian Ene Roată Ștefan Morărașu Ștefan Morărașu Valeri Țuțuianu Marcel Ciobanu Mihail-Gabriel Dimofte Mihail-Gabriel Dimofte |
| author_sort | Irina Cezara Văcărean-Trandafir |
| collection | DOAJ |
| description | IntroductionAntibiotic overuse is driving a global rise in antibiotic resistance, highlighting the need for robust antimicrobial stewardship (AMS) initiatives to improve prescription practices. While antimicrobials are essential for treating sepsis and preventing surgical site infections (SSIs), they can inadvertently disrupt the gut microbiota, leading to postoperative complications. Treatment methods vary widely across nations due to differences in drug choice, dosage, and therapy duration, affecting antibiotic resistance rates, which can reach up to 51% in some countries. In Romania and the Republic of Moldova, healthcare practices for surgical antibiotic prophylaxis differ significantly despite similarities in genetics, culture, and diet. Romania's stricter healthcare regulations result in more standardized antibiotic protocols, whereas Moldova's limited healthcare funding leads to less consistent practices and greater variability in treatment outcomes.MethodsThis study presents the results of a prospective cross-border investigation involving 86 colorectal cancer patients from major oncological hospitals in Romania and Moldova. We analyzed fecal samples collected from patients before and 7 days post-antibiotic treatment, focusing on the V3–V4 region of the 16S rRNA gene.ResultsOur findings indicate that inconsistent antibiotic prophylaxis policies—varying in type, dosage, or therapy duration—significantly impacted the gut microbiota and led to more frequent dysbiosis compared to stricter prophylactic antibiotic practices (single dose, single product, limited time).DiscussionWe emphasize the need for standardized antibiotic prophylaxis protocols to minimize dysbiosis and its associated risks, promoting more effective antimicrobial use, particularly in low- and middle-income countries (LMICs). |
| format | Article |
| id | doaj-art-1b289c8b72cb45f7b30af3aa7262e2c9 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-1b289c8b72cb45f7b30af3aa7262e2c92025-01-13T06:11:04ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.14686451468645Impact of antibiotic prophylaxis on gut microbiota in colorectal surgery: insights from an Eastern European stewardship studyIrina Cezara Văcărean-Trandafir0Roxana-Maria Amărandi1Iuliu Cristian Ivanov2Loredana Mihaiela Dragoș3Mihaela Mențel4Ştefan Iacob5Ştefan Iacob6Ana-Maria Muşină7Ana-Maria Muşină8Elena-Roxana Bărgăoanu9Cristian Ene Roată10Cristian Ene Roată11Ștefan Morărașu12Ștefan Morărașu13Valeri Țuțuianu14Marcel Ciobanu15Mihail-Gabriel Dimofte16Mihail-Gabriel Dimofte17TRANSCEND Research Centre, Regional Institute of Oncology, Iasi, RomaniaTRANSCEND Research Centre, Regional Institute of Oncology, Iasi, RomaniaTRANSCEND Research Centre, Regional Institute of Oncology, Iasi, RomaniaTRANSCEND Research Centre, Regional Institute of Oncology, Iasi, RomaniaTRANSCEND Research Centre, Regional Institute of Oncology, Iasi, RomaniaSecond Surgical Oncology Department, Regional Institute of Oncology, Iasi, RomaniaSurgery Department, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, RomaniaSecond Surgical Oncology Department, Regional Institute of Oncology, Iasi, RomaniaSurgery Department, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, RomaniaSecond Surgical Oncology Department, Regional Institute of Oncology, Iasi, RomaniaSecond Surgical Oncology Department, Regional Institute of Oncology, Iasi, RomaniaSurgery Department, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, RomaniaSecond Surgical Oncology Department, Regional Institute of Oncology, Iasi, RomaniaSurgery Department, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, RomaniaScientific Laboratory of Cancer Biology, Institute of Oncology, Chișinău, MoldovaSurgical Oncology Department, Proctology, Institute of Oncology, Chișinău, MoldovaSecond Surgical Oncology Department, Regional Institute of Oncology, Iasi, RomaniaSurgery Department, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi, RomaniaIntroductionAntibiotic overuse is driving a global rise in antibiotic resistance, highlighting the need for robust antimicrobial stewardship (AMS) initiatives to improve prescription practices. While antimicrobials are essential for treating sepsis and preventing surgical site infections (SSIs), they can inadvertently disrupt the gut microbiota, leading to postoperative complications. Treatment methods vary widely across nations due to differences in drug choice, dosage, and therapy duration, affecting antibiotic resistance rates, which can reach up to 51% in some countries. In Romania and the Republic of Moldova, healthcare practices for surgical antibiotic prophylaxis differ significantly despite similarities in genetics, culture, and diet. Romania's stricter healthcare regulations result in more standardized antibiotic protocols, whereas Moldova's limited healthcare funding leads to less consistent practices and greater variability in treatment outcomes.MethodsThis study presents the results of a prospective cross-border investigation involving 86 colorectal cancer patients from major oncological hospitals in Romania and Moldova. We analyzed fecal samples collected from patients before and 7 days post-antibiotic treatment, focusing on the V3–V4 region of the 16S rRNA gene.ResultsOur findings indicate that inconsistent antibiotic prophylaxis policies—varying in type, dosage, or therapy duration—significantly impacted the gut microbiota and led to more frequent dysbiosis compared to stricter prophylactic antibiotic practices (single dose, single product, limited time).DiscussionWe emphasize the need for standardized antibiotic prophylaxis protocols to minimize dysbiosis and its associated risks, promoting more effective antimicrobial use, particularly in low- and middle-income countries (LMICs).https://www.frontiersin.org/articles/10.3389/fcimb.2024.1468645/fullgut microbiotacolorectal cancerantibiotic therapy16S rRNA NGSASPAMS |
| spellingShingle | Irina Cezara Văcărean-Trandafir Roxana-Maria Amărandi Iuliu Cristian Ivanov Loredana Mihaiela Dragoș Mihaela Mențel Ştefan Iacob Ştefan Iacob Ana-Maria Muşină Ana-Maria Muşină Elena-Roxana Bărgăoanu Cristian Ene Roată Cristian Ene Roată Ștefan Morărașu Ștefan Morărașu Valeri Țuțuianu Marcel Ciobanu Mihail-Gabriel Dimofte Mihail-Gabriel Dimofte Impact of antibiotic prophylaxis on gut microbiota in colorectal surgery: insights from an Eastern European stewardship study Frontiers in Cellular and Infection Microbiology gut microbiota colorectal cancer antibiotic therapy 16S rRNA NGS ASP AMS |
| title | Impact of antibiotic prophylaxis on gut microbiota in colorectal surgery: insights from an Eastern European stewardship study |
| title_full | Impact of antibiotic prophylaxis on gut microbiota in colorectal surgery: insights from an Eastern European stewardship study |
| title_fullStr | Impact of antibiotic prophylaxis on gut microbiota in colorectal surgery: insights from an Eastern European stewardship study |
| title_full_unstemmed | Impact of antibiotic prophylaxis on gut microbiota in colorectal surgery: insights from an Eastern European stewardship study |
| title_short | Impact of antibiotic prophylaxis on gut microbiota in colorectal surgery: insights from an Eastern European stewardship study |
| title_sort | impact of antibiotic prophylaxis on gut microbiota in colorectal surgery insights from an eastern european stewardship study |
| topic | gut microbiota colorectal cancer antibiotic therapy 16S rRNA NGS ASP AMS |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1468645/full |
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