Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells
Combination of stem cell technology and 3D biofabrication approaches provides physiological similarity to in vivo tissues and the capability of repairing and regenerating damaged human tissues. Mesenchymal stem cells (MSCs) have been widely used for regenerative medicine applications because of thei...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/5654324 |
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| _version_ | 1841524515175137280 |
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| author | Tackla S. Winston Kantaphon Suddhapas Chenyan Wang Rafael Ramos Pranav Soman Zhen Ma |
| author_facet | Tackla S. Winston Kantaphon Suddhapas Chenyan Wang Rafael Ramos Pranav Soman Zhen Ma |
| author_sort | Tackla S. Winston |
| collection | DOAJ |
| description | Combination of stem cell technology and 3D biofabrication approaches provides physiological similarity to in vivo tissues and the capability of repairing and regenerating damaged human tissues. Mesenchymal stem cells (MSCs) have been widely used for regenerative medicine applications because of their immunosuppressive properties and multipotent potentials. To obtain large amount of high-quality MSCs without patient donation and invasive procedures, we differentiated MSCs from human-induced pluripotent stem cells (hiPSC-MSCs) using serum-free E6 media supplemented with only one growth factor (bFGF) and two small molecules (SB431542 and CHIR99021). The differentiated cells showed a high expression of common MSC-specific surface markers (CD90, CD73, CD105, CD106, CD146, and CD166) and a high potency for osteogenic and chondrogenic differentiation. With these cells, we have been able to manufacture MSC tissue rings with high consistency and robustness in pluronic-coated reusable PDMS devices. The MSC tissue rings were characterized based on inner diameter and outer ring diameter and observed cell-type-dependent tissue contraction induced by cell-matrix interaction. Our approach of simplified hiPSC-MSC differentiation, modular fabrication procedure, and serum-free culture conditions has a great potential for scalable manufacturing of MSC tissue rings for different regenerative medicine applications. |
| format | Article |
| id | doaj-art-1b229c07ab6b47609aa883c9ea78a88d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-1b229c07ab6b47609aa883c9ea78a88d2025-02-03T05:53:01ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/56543245654324Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem CellsTackla S. Winston0Kantaphon Suddhapas1Chenyan Wang2Rafael Ramos3Pranav Soman4Zhen Ma5Department of Biomedical & Chemical Engineering, Syracuse University, Syracuse NY 13244, USADepartment of Biomedical & Chemical Engineering, Syracuse University, Syracuse NY 13244, USADepartment of Biomedical & Chemical Engineering, Syracuse University, Syracuse NY 13244, USADepartment of Biomedical & Chemical Engineering, Syracuse University, Syracuse NY 13244, USADepartment of Biomedical & Chemical Engineering, Syracuse University, Syracuse NY 13244, USADepartment of Biomedical & Chemical Engineering, Syracuse University, Syracuse NY 13244, USACombination of stem cell technology and 3D biofabrication approaches provides physiological similarity to in vivo tissues and the capability of repairing and regenerating damaged human tissues. Mesenchymal stem cells (MSCs) have been widely used for regenerative medicine applications because of their immunosuppressive properties and multipotent potentials. To obtain large amount of high-quality MSCs without patient donation and invasive procedures, we differentiated MSCs from human-induced pluripotent stem cells (hiPSC-MSCs) using serum-free E6 media supplemented with only one growth factor (bFGF) and two small molecules (SB431542 and CHIR99021). The differentiated cells showed a high expression of common MSC-specific surface markers (CD90, CD73, CD105, CD106, CD146, and CD166) and a high potency for osteogenic and chondrogenic differentiation. With these cells, we have been able to manufacture MSC tissue rings with high consistency and robustness in pluronic-coated reusable PDMS devices. The MSC tissue rings were characterized based on inner diameter and outer ring diameter and observed cell-type-dependent tissue contraction induced by cell-matrix interaction. Our approach of simplified hiPSC-MSC differentiation, modular fabrication procedure, and serum-free culture conditions has a great potential for scalable manufacturing of MSC tissue rings for different regenerative medicine applications.http://dx.doi.org/10.1155/2019/5654324 |
| spellingShingle | Tackla S. Winston Kantaphon Suddhapas Chenyan Wang Rafael Ramos Pranav Soman Zhen Ma Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells Stem Cells International |
| title | Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells |
| title_full | Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells |
| title_fullStr | Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells |
| title_full_unstemmed | Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells |
| title_short | Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells |
| title_sort | serum free manufacturing of mesenchymal stem cell tissue rings using human induced pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2019/5654324 |
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