OsEDS1 and OsPAD4 Are Involved in Brown Planthopper Resistance in Rice
The crucial roles of the lipase-like protein enhanced disease susceptibility 1 (EDS1) and phytoalexin deficient 4 (PAD4) in disease resistance in <i>Arabidopsis</i> have been identified. However, their function in rice (<i>Oryza sativa</i> L.) resistance to brown planthopper...
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2025-05-01
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| author | Linzhi Fang Rong Su Cunyan Li Xiaodong Liu Yuanyuan Song Rensen Zeng Qiongli Wang Haitao Cui Daoqian Chen |
| author_facet | Linzhi Fang Rong Su Cunyan Li Xiaodong Liu Yuanyuan Song Rensen Zeng Qiongli Wang Haitao Cui Daoqian Chen |
| author_sort | Linzhi Fang |
| collection | DOAJ |
| description | The crucial roles of the lipase-like protein enhanced disease susceptibility 1 (EDS1) and phytoalexin deficient 4 (PAD4) in disease resistance in <i>Arabidopsis</i> have been identified. However, their function in rice (<i>Oryza sativa</i> L.) resistance to brown planthopper (BPH, <i>Nilaparvata lugens</i> Stål), the most notorious pest of rice, remains unknown. In this study, the transcript levels of <i>OsEDS1</i> and <i>OsPAD4</i> were rapidly altered by BPH infestation. Mutation in either <i>OsPAD4</i> or <i>OsEDS1</i> resulted in increased rice susceptibility to BPH, which was associated with increased honeydew excretion and an increased host preference of BPH. Furthermore, mutation in either <i>OsPAD4</i> or <i>OsEDS1</i> led to decreased basal levels of salicylic acid (SA) and jasmonic acid (JA) in the absence of BPH, along with the depressed expression of the defense-responsive genes <i>OsPAL</i>, <i>OsICS1</i>, <i>OsPR1a</i>, <i>OsLOX1</i>, <i>OsAOS1</i> and <i>OsJAZ11</i> involved in SA and JA biosynthesis and signaling. The BPH infestation-mediated elevation of SA levels and the expression of SA biosynthesis and signaling genes was dampened in <i>eds1</i> and <i>pad4</i> plants, whereas BPH infestation-mediated depressions of JA levels and the expression of JA biosynthesis and signaling genes were reversed in <i>eds1</i> and <i>pad4</i> plants. Taken together, our findings indicated that both OsPAD4 and OsEDS1 positively regulate rice resistance to BPH. |
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| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Plants |
| spelling | doaj-art-1b0a2e06ec2140e3969b2de4c98e1f262025-08-20T03:46:49ZengMDPI AGPlants2223-77472025-05-011411161210.3390/plants14111612OsEDS1 and OsPAD4 Are Involved in Brown Planthopper Resistance in RiceLinzhi Fang0Rong Su1Cunyan Li2Xiaodong Liu3Yuanyuan Song4Rensen Zeng5Qiongli Wang6Haitao Cui7Daoqian Chen8Key Laboratory of Ministry of Education for Genetics, State Key Laboratory of Agricultural and Forestry Biosecurity, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, State Key Laboratory of Agricultural and Forestry Biosecurity, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, State Key Laboratory of Agricultural and Forestry Biosecurity, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, State Key Laboratory of Agricultural and Forestry Biosecurity, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, State Key Laboratory of Agricultural and Forestry Biosecurity, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, State Key Laboratory of Agricultural and Forestry Biosecurity, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, State Key Laboratory of Agricultural and Forestry Biosecurity, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Plant Protection, Shandong Agricultural University, Taian 271000, ChinaKey Laboratory of Ministry of Education for Genetics, State Key Laboratory of Agricultural and Forestry Biosecurity, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThe crucial roles of the lipase-like protein enhanced disease susceptibility 1 (EDS1) and phytoalexin deficient 4 (PAD4) in disease resistance in <i>Arabidopsis</i> have been identified. However, their function in rice (<i>Oryza sativa</i> L.) resistance to brown planthopper (BPH, <i>Nilaparvata lugens</i> Stål), the most notorious pest of rice, remains unknown. In this study, the transcript levels of <i>OsEDS1</i> and <i>OsPAD4</i> were rapidly altered by BPH infestation. Mutation in either <i>OsPAD4</i> or <i>OsEDS1</i> resulted in increased rice susceptibility to BPH, which was associated with increased honeydew excretion and an increased host preference of BPH. Furthermore, mutation in either <i>OsPAD4</i> or <i>OsEDS1</i> led to decreased basal levels of salicylic acid (SA) and jasmonic acid (JA) in the absence of BPH, along with the depressed expression of the defense-responsive genes <i>OsPAL</i>, <i>OsICS1</i>, <i>OsPR1a</i>, <i>OsLOX1</i>, <i>OsAOS1</i> and <i>OsJAZ11</i> involved in SA and JA biosynthesis and signaling. The BPH infestation-mediated elevation of SA levels and the expression of SA biosynthesis and signaling genes was dampened in <i>eds1</i> and <i>pad4</i> plants, whereas BPH infestation-mediated depressions of JA levels and the expression of JA biosynthesis and signaling genes were reversed in <i>eds1</i> and <i>pad4</i> plants. Taken together, our findings indicated that both OsPAD4 and OsEDS1 positively regulate rice resistance to BPH.https://www.mdpi.com/2223-7747/14/11/1612OsPAD4OsEDS1<i>Oryza sativa</i>brown planthopper |
| spellingShingle | Linzhi Fang Rong Su Cunyan Li Xiaodong Liu Yuanyuan Song Rensen Zeng Qiongli Wang Haitao Cui Daoqian Chen OsEDS1 and OsPAD4 Are Involved in Brown Planthopper Resistance in Rice Plants OsPAD4 OsEDS1 <i>Oryza sativa</i> brown planthopper |
| title | OsEDS1 and OsPAD4 Are Involved in Brown Planthopper Resistance in Rice |
| title_full | OsEDS1 and OsPAD4 Are Involved in Brown Planthopper Resistance in Rice |
| title_fullStr | OsEDS1 and OsPAD4 Are Involved in Brown Planthopper Resistance in Rice |
| title_full_unstemmed | OsEDS1 and OsPAD4 Are Involved in Brown Planthopper Resistance in Rice |
| title_short | OsEDS1 and OsPAD4 Are Involved in Brown Planthopper Resistance in Rice |
| title_sort | oseds1 and ospad4 are involved in brown planthopper resistance in rice |
| topic | OsPAD4 OsEDS1 <i>Oryza sativa</i> brown planthopper |
| url | https://www.mdpi.com/2223-7747/14/11/1612 |
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