The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma
Abstract Background: Abnormal expression of Zinc finger (ZNF) genes is commonly observed in osteosarcoma (OS), the most prevalent malignant bone tumor in children and teenagers. This project focused on the role of ZNF560 in the progress of OS. Methods: The published datasets including TCGA-SARC and...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-79298-y |
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| author | Xiong Dong Guanhua Xu Hongxiang Hong Jinlong Zhang Zhiming Cui ZiLiang Yu |
| author_facet | Xiong Dong Guanhua Xu Hongxiang Hong Jinlong Zhang Zhiming Cui ZiLiang Yu |
| author_sort | Xiong Dong |
| collection | DOAJ |
| description | Abstract Background: Abnormal expression of Zinc finger (ZNF) genes is commonly observed in osteosarcoma (OS), the most prevalent malignant bone tumor in children and teenagers. This project focused on the role of ZNF560 in the progress of OS. Methods: The published datasets including TCGA-SARC and GSE99671 was utilized to screen out the abnormal expression of ZNF560 and associated gene patterns in sarcoma and OS tissues. Prognosis value of ZNF560 was identified in TCGA-SARC and OS cohorts. In order to manipulate ZNF560 expression in HOS and MG63 osteosarcoma (OS) cells, genetic strategies such as shRNA constructs were utilized. The expression patterns of ZNF560 were analyzed through techniques such as immunohistochemistry, Western blotting, and qRT-PCR. Results: By analyzing data from both the GEO and the Cancer Genome Atlas (TCGA) databases, increased expression of ZNF560 in OS tissues was verified, which was significantly associated with poorer outcomes in osteosarcoma patients both in TCGA-SARC and our own OS cohorts. Additionally, downregulation of ZNF560 resulted in decreased cell viability, fewer colonies, and induced apoptosis of osteosarcoma cells. Moreover, ZNF560 was found to be essential for migration of human osteosarcoma HOS and MG63 cells. Conclusion: Collectively, these findings suggest that ZNF560 has the potential to serve as a predictive biomarker for osteosarcoma. |
| format | Article |
| id | doaj-art-1ad071d3e26f427d94247793defaf491 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
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| spelling | doaj-art-1ad071d3e26f427d94247793defaf4912025-01-05T12:14:03ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-024-79298-yThe zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcomaXiong Dong0Guanhua Xu1Hongxiang Hong2Jinlong Zhang3Zhiming Cui4ZiLiang Yu5Spinal Surgery Department, The Second Affiliated Hospital of Nantong UniversitySpinal Surgery Department, The Second Affiliated Hospital of Nantong UniversitySpinal Surgery Department, The Second Affiliated Hospital of Nantong UniversitySpinal Surgery Department, The Second Affiliated Hospital of Nantong UniversitySpinal Surgery Department, The Second Affiliated Hospital of Nantong UniversityDepartment of Joint Surgery, The Second Affiliated Hospital of Nantong UniversityAbstract Background: Abnormal expression of Zinc finger (ZNF) genes is commonly observed in osteosarcoma (OS), the most prevalent malignant bone tumor in children and teenagers. This project focused on the role of ZNF560 in the progress of OS. Methods: The published datasets including TCGA-SARC and GSE99671 was utilized to screen out the abnormal expression of ZNF560 and associated gene patterns in sarcoma and OS tissues. Prognosis value of ZNF560 was identified in TCGA-SARC and OS cohorts. In order to manipulate ZNF560 expression in HOS and MG63 osteosarcoma (OS) cells, genetic strategies such as shRNA constructs were utilized. The expression patterns of ZNF560 were analyzed through techniques such as immunohistochemistry, Western blotting, and qRT-PCR. Results: By analyzing data from both the GEO and the Cancer Genome Atlas (TCGA) databases, increased expression of ZNF560 in OS tissues was verified, which was significantly associated with poorer outcomes in osteosarcoma patients both in TCGA-SARC and our own OS cohorts. Additionally, downregulation of ZNF560 resulted in decreased cell viability, fewer colonies, and induced apoptosis of osteosarcoma cells. Moreover, ZNF560 was found to be essential for migration of human osteosarcoma HOS and MG63 cells. Conclusion: Collectively, these findings suggest that ZNF560 has the potential to serve as a predictive biomarker for osteosarcoma.https://doi.org/10.1038/s41598-024-79298-yOsteosarcomaZNF560BiomarkerTumorigenesisPrognosis |
| spellingShingle | Xiong Dong Guanhua Xu Hongxiang Hong Jinlong Zhang Zhiming Cui ZiLiang Yu The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma Scientific Reports Osteosarcoma ZNF560 Biomarker Tumorigenesis Prognosis |
| title | The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma |
| title_full | The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma |
| title_fullStr | The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma |
| title_full_unstemmed | The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma |
| title_short | The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma |
| title_sort | zinc finger protein560 znf560 functions as a novel oncogenic gene in osteosarcoma |
| topic | Osteosarcoma ZNF560 Biomarker Tumorigenesis Prognosis |
| url | https://doi.org/10.1038/s41598-024-79298-y |
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