Studies on the fertilization of mouse eggs in vitro
The aim of this study was to test the recommended techniques of in vitro fertilization in Iriice as an experimental animaL<p> Adult female mice of Swiss albino strain were superovulated by intrapeıitonal injection of PMSG and HCG 48 hours apart. They were killed 16 hours after injection of HCG...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=720 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The aim of this study was to test
the recommended techniques of in vitro fertilization
in Iriice as an experimental animaL<p>
Adult female mice of Swiss albino strain
were superovulated by intrapeıitonal injection of
PMSG and HCG 48 hours apart. They were killed
16 hours after injection of HCG and their eggs
were recovered under warm oil in a plastic dish
containing 0.4 ml of ineubatian medium.<p>
Sperms recovered from cauda epididymis of
adult males of the same strairi were suspended in
a medtum and incubated at 37° C under % 5 CO<sub>2</sub>
in air for 1.5-2 hours before they were used for insemination.
The ineubatian medium was THY medium
containing glucose, sodium pyruvate, bovine
albumtn and antibiotics.<p>
At the time of insemination, a drop of sperm
suspension (10-20 µl) was added to the medium
contatning egg clot. Eggs and sperms were incubated
in ineubatian medium for 6 hours. In the fırst
group, fertilized eggs were left into the same medium.
In the second group they were transferred to
the development medium (Modifıed Whitten's) without
sodium lactate and incubated until they were
examtned under stereo-microscope.<p>
As a conclusion, in this study fertilized eggs
and 2-cell stage embıyos were obtained but further
stage of embıyos (morulae, blastocyst) couldn't be
reached. |
|---|---|
| ISSN: | 1309-6958 2146-1953 |