Effect of In Vitro Pretreatment with Ag-Containing Amino Acid Nanofibers on Biometrics and Antioxidant Activity in Drought-Stressed Ex Vitro-Adapted <i>Stevia rebaudiana</i> Bertoni
Biotechnological methods prevent the destruction of natural populations of medicinal plants due to climate change and developing agriculture. This study evaluates the effects of in vitro pretreatment with two types of silver-containing amino acid nanofibers (NF-1%Ag and NF1-Ag salt) on the drought t...
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2024-11-01
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| author | Kamelia Miladinova-Georgieva Mariana Sichanova Maria Petrova Elisaveta Kirova Trendafil Nedev Daniela Tsekova Maria Geneva |
| author_facet | Kamelia Miladinova-Georgieva Mariana Sichanova Maria Petrova Elisaveta Kirova Trendafil Nedev Daniela Tsekova Maria Geneva |
| author_sort | Kamelia Miladinova-Georgieva |
| collection | DOAJ |
| description | Biotechnological methods prevent the destruction of natural populations of medicinal plants due to climate change and developing agriculture. This study evaluates the effects of in vitro pretreatment with two types of silver-containing amino acid nanofibers (NF-1%Ag and NF1-Ag salt) on the drought tolerance of ex vitro soil-adapted <i>Steviia rebaudiana</i> Bertoni. The duration of the drought was five days. The data suggested that the pretreatment with the studied nanofibers during plant propagation enhanced the plant tolerance to drought stress manifested in a smaller decrease in plant biomass accumulation and a smaller increase in sugar content. The pretreatment with the two tested nanoparticles of well-watered plants increased the leaf fresh biomass accumulation of the ex vitro-adapted <i>S. rebaudiana</i> compared to the untreated WW control plants. The highest values were reported at 10 mg L<sup>−1</sup> NF1-Ag salt. Five days of drought led to a decrease in the leaf fresh biomass compared to the WW plants, with the recorded lowest reduction again at 10 mg L<sup>−1</sup> NF1-Ag salt. These observations correlate with antioxidant activity improvement. The results show that adding 10 mg L<sup>−1</sup> NF1-Ag salt to the MS medium led to higher ex vitro-adapted <i>S. rebaudiana</i> resistance to water deficit than 100 mg L<sup>−1</sup>. This paper discusses the impact of the selected nanofibers on parameters characterizing plant growth and antioxidant activity of drought-stressed ex vitro-adapted <i>Stevia rebaudiana</i> plants. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
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| series | Agronomy |
| spelling | doaj-art-1aace7dc953c426cbf92a2ccdf2974312024-11-26T17:44:29ZengMDPI AGAgronomy2073-43952024-11-011411257010.3390/agronomy14112570Effect of In Vitro Pretreatment with Ag-Containing Amino Acid Nanofibers on Biometrics and Antioxidant Activity in Drought-Stressed Ex Vitro-Adapted <i>Stevia rebaudiana</i> BertoniKamelia Miladinova-Georgieva0Mariana Sichanova1Maria Petrova2Elisaveta Kirova3Trendafil Nedev4Daniela Tsekova5Maria Geneva6Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, 1113 Sofia, BulgariaInstitute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, 1113 Sofia, BulgariaInstitute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, 1113 Sofia, BulgariaInstitute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, 1113 Sofia, BulgariaInstitute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, 1113 Sofia, BulgariaDepartment of Organic Chemistry, University of Chemical Technology and Metallurgy, 8 “St. Kl. Ohridski” Blvd, 1756 Sofia, BulgariaInstitute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, 1113 Sofia, BulgariaBiotechnological methods prevent the destruction of natural populations of medicinal plants due to climate change and developing agriculture. This study evaluates the effects of in vitro pretreatment with two types of silver-containing amino acid nanofibers (NF-1%Ag and NF1-Ag salt) on the drought tolerance of ex vitro soil-adapted <i>Steviia rebaudiana</i> Bertoni. The duration of the drought was five days. The data suggested that the pretreatment with the studied nanofibers during plant propagation enhanced the plant tolerance to drought stress manifested in a smaller decrease in plant biomass accumulation and a smaller increase in sugar content. The pretreatment with the two tested nanoparticles of well-watered plants increased the leaf fresh biomass accumulation of the ex vitro-adapted <i>S. rebaudiana</i> compared to the untreated WW control plants. The highest values were reported at 10 mg L<sup>−1</sup> NF1-Ag salt. Five days of drought led to a decrease in the leaf fresh biomass compared to the WW plants, with the recorded lowest reduction again at 10 mg L<sup>−1</sup> NF1-Ag salt. These observations correlate with antioxidant activity improvement. The results show that adding 10 mg L<sup>−1</sup> NF1-Ag salt to the MS medium led to higher ex vitro-adapted <i>S. rebaudiana</i> resistance to water deficit than 100 mg L<sup>−1</sup>. This paper discusses the impact of the selected nanofibers on parameters characterizing plant growth and antioxidant activity of drought-stressed ex vitro-adapted <i>Stevia rebaudiana</i> plants.https://www.mdpi.com/2073-4395/14/11/2570<i>Stevia rebaudiana</i> Bert.droughtbiomass accumulationantioxidant activity |
| spellingShingle | Kamelia Miladinova-Georgieva Mariana Sichanova Maria Petrova Elisaveta Kirova Trendafil Nedev Daniela Tsekova Maria Geneva Effect of In Vitro Pretreatment with Ag-Containing Amino Acid Nanofibers on Biometrics and Antioxidant Activity in Drought-Stressed Ex Vitro-Adapted <i>Stevia rebaudiana</i> Bertoni Agronomy <i>Stevia rebaudiana</i> Bert. drought biomass accumulation antioxidant activity |
| title | Effect of In Vitro Pretreatment with Ag-Containing Amino Acid Nanofibers on Biometrics and Antioxidant Activity in Drought-Stressed Ex Vitro-Adapted <i>Stevia rebaudiana</i> Bertoni |
| title_full | Effect of In Vitro Pretreatment with Ag-Containing Amino Acid Nanofibers on Biometrics and Antioxidant Activity in Drought-Stressed Ex Vitro-Adapted <i>Stevia rebaudiana</i> Bertoni |
| title_fullStr | Effect of In Vitro Pretreatment with Ag-Containing Amino Acid Nanofibers on Biometrics and Antioxidant Activity in Drought-Stressed Ex Vitro-Adapted <i>Stevia rebaudiana</i> Bertoni |
| title_full_unstemmed | Effect of In Vitro Pretreatment with Ag-Containing Amino Acid Nanofibers on Biometrics and Antioxidant Activity in Drought-Stressed Ex Vitro-Adapted <i>Stevia rebaudiana</i> Bertoni |
| title_short | Effect of In Vitro Pretreatment with Ag-Containing Amino Acid Nanofibers on Biometrics and Antioxidant Activity in Drought-Stressed Ex Vitro-Adapted <i>Stevia rebaudiana</i> Bertoni |
| title_sort | effect of in vitro pretreatment with ag containing amino acid nanofibers on biometrics and antioxidant activity in drought stressed ex vitro adapted i stevia rebaudiana i bertoni |
| topic | <i>Stevia rebaudiana</i> Bert. drought biomass accumulation antioxidant activity |
| url | https://www.mdpi.com/2073-4395/14/11/2570 |
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