Isolation and Enumeration of Bradyrhizobium Species Dwelling In the Root Nodules of Soybean Plant
Biological Nitrogen fixation is one of the important aspects of organic agriculture gaining considerable attention globally. Information about the number of viable indigenous Bradyrhizobia in soils planted with legumes and their capacity to nodulate is a valuable tool in developing strategies to im...
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Umaru Musa Yar'adua University, Katsina, Nigeria
2020-12-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/132 |
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| author | Ishaq, Z. Machido, D.A. Atta, H.I. |
| author_facet | Ishaq, Z. Machido, D.A. Atta, H.I. |
| author_sort | Ishaq, Z. |
| collection | DOAJ |
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Biological Nitrogen fixation is one of the important aspects of organic agriculture gaining considerable attention globally. Information about the number of viable indigenous Bradyrhizobia in soils planted with legumes and their capacity to nodulate is a valuable tool in developing strategies to improve biological nitrogen fixation. Such strategies could potentially lead to increased soybean yields at low cost. This study was conducted to isolate and enumerate Bradyrizobium species dwelling in the root nodule of soybean plant using Bradyrhizobium japonicum selective medium (BJSM). Twenty (20) strains of Bradyrizobium species were isolated from the root nodules of soybean plants harvested from Ahmadu Bello University farm site, located at Bomo district of Sabongari local government area, Kaduna State, Nigeria. This was achieved using the streak method of isolation on BJSM. Ninety percent (18) of these isolates were confirmed as Bradyrizobium species using the plant infection test as they were able to nodulate the roots of soybean plants. The enumeration of the indigenous Bradyrizobium species gave a count ranging from 2.07x105 - 4.0x106 CFU/mL. Thus, the number of Bradyrhizobia obtained in the soil of this study is sufficient to achieve satisfactory results on nodulation and nitrogen fixation.
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| format | Article |
| id | doaj-art-1aa1522b1923424fa22a7be4f4cc739c |
| institution | Kabale University |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-1aa1522b1923424fa22a7be4f4cc739c2024-11-28T11:07:32ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222020-12-015210.47430/ujmr.2052.003132Isolation and Enumeration of Bradyrhizobium Species Dwelling In the Root Nodules of Soybean PlantIshaq, Z.0Machido, D.A.1Atta, H.I.2Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria. Biological Nitrogen fixation is one of the important aspects of organic agriculture gaining considerable attention globally. Information about the number of viable indigenous Bradyrhizobia in soils planted with legumes and their capacity to nodulate is a valuable tool in developing strategies to improve biological nitrogen fixation. Such strategies could potentially lead to increased soybean yields at low cost. This study was conducted to isolate and enumerate Bradyrizobium species dwelling in the root nodule of soybean plant using Bradyrhizobium japonicum selective medium (BJSM). Twenty (20) strains of Bradyrizobium species were isolated from the root nodules of soybean plants harvested from Ahmadu Bello University farm site, located at Bomo district of Sabongari local government area, Kaduna State, Nigeria. This was achieved using the streak method of isolation on BJSM. Ninety percent (18) of these isolates were confirmed as Bradyrizobium species using the plant infection test as they were able to nodulate the roots of soybean plants. The enumeration of the indigenous Bradyrizobium species gave a count ranging from 2.07x105 - 4.0x106 CFU/mL. Thus, the number of Bradyrhizobia obtained in the soil of this study is sufficient to achieve satisfactory results on nodulation and nitrogen fixation. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/132SoybeanBradyrhizobium speciesNodulationNitrogen fixation |
| spellingShingle | Ishaq, Z. Machido, D.A. Atta, H.I. Isolation and Enumeration of Bradyrhizobium Species Dwelling In the Root Nodules of Soybean Plant UMYU Journal of Microbiology Research Soybean Bradyrhizobium species Nodulation Nitrogen fixation |
| title | Isolation and Enumeration of Bradyrhizobium Species Dwelling In the Root Nodules of Soybean Plant |
| title_full | Isolation and Enumeration of Bradyrhizobium Species Dwelling In the Root Nodules of Soybean Plant |
| title_fullStr | Isolation and Enumeration of Bradyrhizobium Species Dwelling In the Root Nodules of Soybean Plant |
| title_full_unstemmed | Isolation and Enumeration of Bradyrhizobium Species Dwelling In the Root Nodules of Soybean Plant |
| title_short | Isolation and Enumeration of Bradyrhizobium Species Dwelling In the Root Nodules of Soybean Plant |
| title_sort | isolation and enumeration of bradyrhizobium species dwelling in the root nodules of soybean plant |
| topic | Soybean Bradyrhizobium species Nodulation Nitrogen fixation |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/132 |
| work_keys_str_mv | AT ishaqz isolationandenumerationofbradyrhizobiumspeciesdwellingintherootnodulesofsoybeanplant AT machidoda isolationandenumerationofbradyrhizobiumspeciesdwellingintherootnodulesofsoybeanplant AT attahi isolationandenumerationofbradyrhizobiumspeciesdwellingintherootnodulesofsoybeanplant |