The bioinfomatics analysis of the M1 macrophage-related gene CXCL9 signature in cervical cancer
Background The expression and function of coexpression genes of M1 macrophage in cervical cancer have not been identified. And the CXCL9-expressing tumour-associated macrophage has been poorly reported in cervical cancer.Methods To clarify the regulatory gene network of M1 macrophage in cervical can...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Obstetrics and Gynaecology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01443615.2024.2373951 |
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| author | Wenxin Liao Tingting Liu Yang Li Hua Liang Juexiao Deng Fujin Shen |
| author_facet | Wenxin Liao Tingting Liu Yang Li Hua Liang Juexiao Deng Fujin Shen |
| author_sort | Wenxin Liao |
| collection | DOAJ |
| description | Background The expression and function of coexpression genes of M1 macrophage in cervical cancer have not been identified. And the CXCL9-expressing tumour-associated macrophage has been poorly reported in cervical cancer.Methods To clarify the regulatory gene network of M1 macrophage in cervical cancer, we downloaded gene expression profiles of cervical cancer patients in TCGA database to identify M1 macrophage coexpression genes. Then we constructed the protein–protein interaction networks by STRING database and performed functional enrichment analysis to investigate the biological effects of the coexpression genes. Next, we used multiple bioinformatics databases and experiments to overall investigate coexpression gene CXCL9, including western blot assay and immunohistochemistry assay, GeneMANIA, Kaplan-Meier Plotter, Xenashiny, TISCH2, ACLBI, HPA, TISIDB, GSCA and cBioPortal databases.Results There were 77 positive coexpression genes and 5 negative coexpression genes in M1 macrophage. The coexpression genes in M1 macrophage participated in the production and function of chemokines and chemokine receptors. Especially, CXCL9 was positively correlated with M1 macrophage infiltration levels in cervical cancer. CXCL9 expression would significantly decrease and high CXCL9 levels were linked to good prognosis in the cervical cancer tumour patients, it manifestly expressed in blood immune cells, and was positively related to immune checkpoints. CXCL9 amplification was the most common type of mutation. The CXCL9 gene interaction network could regulate immune-related signalling pathways, and CXCL9 amplification was the most common mutation type in cervical cancer. Meanwhile, CXCL9 may had clinical significance for the drug response in cervical cancer, possibly mediating resistance to chemotherapy and targeted drug therapy.Conclusion Our findings may provide new insight into the M1 macrophage coexpression gene network and molecular mechanisms in cervical cancer, and indicated that M1 macrophage association gene CXCL9 may serve as a good prognostic gene and a potential therapeutic target for cervical cancer therapies. |
| format | Article |
| id | doaj-art-1a93d3ec29ff4cfdac7aee91f93213cf |
| institution | Kabale University |
| issn | 0144-3615 1364-6893 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Obstetrics and Gynaecology |
| spelling | doaj-art-1a93d3ec29ff4cfdac7aee91f93213cf2025-01-09T12:13:17ZengTaylor & Francis GroupJournal of Obstetrics and Gynaecology0144-36151364-68932024-12-0144110.1080/01443615.2024.2373951The bioinfomatics analysis of the M1 macrophage-related gene CXCL9 signature in cervical cancerWenxin Liao0Tingting Liu1Yang Li2Hua Liang3Juexiao Deng4Fujin Shen5Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, ChinaDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, ChinaBackground The expression and function of coexpression genes of M1 macrophage in cervical cancer have not been identified. And the CXCL9-expressing tumour-associated macrophage has been poorly reported in cervical cancer.Methods To clarify the regulatory gene network of M1 macrophage in cervical cancer, we downloaded gene expression profiles of cervical cancer patients in TCGA database to identify M1 macrophage coexpression genes. Then we constructed the protein–protein interaction networks by STRING database and performed functional enrichment analysis to investigate the biological effects of the coexpression genes. Next, we used multiple bioinformatics databases and experiments to overall investigate coexpression gene CXCL9, including western blot assay and immunohistochemistry assay, GeneMANIA, Kaplan-Meier Plotter, Xenashiny, TISCH2, ACLBI, HPA, TISIDB, GSCA and cBioPortal databases.Results There were 77 positive coexpression genes and 5 negative coexpression genes in M1 macrophage. The coexpression genes in M1 macrophage participated in the production and function of chemokines and chemokine receptors. Especially, CXCL9 was positively correlated with M1 macrophage infiltration levels in cervical cancer. CXCL9 expression would significantly decrease and high CXCL9 levels were linked to good prognosis in the cervical cancer tumour patients, it manifestly expressed in blood immune cells, and was positively related to immune checkpoints. CXCL9 amplification was the most common type of mutation. The CXCL9 gene interaction network could regulate immune-related signalling pathways, and CXCL9 amplification was the most common mutation type in cervical cancer. Meanwhile, CXCL9 may had clinical significance for the drug response in cervical cancer, possibly mediating resistance to chemotherapy and targeted drug therapy.Conclusion Our findings may provide new insight into the M1 macrophage coexpression gene network and molecular mechanisms in cervical cancer, and indicated that M1 macrophage association gene CXCL9 may serve as a good prognostic gene and a potential therapeutic target for cervical cancer therapies.https://www.tandfonline.com/doi/10.1080/01443615.2024.2373951M1 macrophagecoexpression genechemokineCXCL9cervical cancer |
| spellingShingle | Wenxin Liao Tingting Liu Yang Li Hua Liang Juexiao Deng Fujin Shen The bioinfomatics analysis of the M1 macrophage-related gene CXCL9 signature in cervical cancer Journal of Obstetrics and Gynaecology M1 macrophage coexpression gene chemokine CXCL9 cervical cancer |
| title | The bioinfomatics analysis of the M1 macrophage-related gene CXCL9 signature in cervical cancer |
| title_full | The bioinfomatics analysis of the M1 macrophage-related gene CXCL9 signature in cervical cancer |
| title_fullStr | The bioinfomatics analysis of the M1 macrophage-related gene CXCL9 signature in cervical cancer |
| title_full_unstemmed | The bioinfomatics analysis of the M1 macrophage-related gene CXCL9 signature in cervical cancer |
| title_short | The bioinfomatics analysis of the M1 macrophage-related gene CXCL9 signature in cervical cancer |
| title_sort | bioinfomatics analysis of the m1 macrophage related gene cxcl9 signature in cervical cancer |
| topic | M1 macrophage coexpression gene chemokine CXCL9 cervical cancer |
| url | https://www.tandfonline.com/doi/10.1080/01443615.2024.2373951 |
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